Construct: sgRNA BRDN0001145270
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATCTCTCTACAAGCCGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK19 (23097)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76504
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
110746146 |
+ |
CDK19 |
NNNCTCTCTACAAGCCGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
5641882 |
- |
SAFB |
NNNCTCTCTACTATCCGACA |
NGG |
2 |
0.0889 |
Tier I |
| 3 |
NC_000002.12 |
2 |
134292445 |
+ |
MGAT5 |
NNNCTCTCTTCAATCCGACA |
NGG |
2 |
0.2353 |
Tier II |
| 4 |
NC_000004.12 |
4 |
71112666 |
- |
SLC4A4 |
NNNTTCTCTACAAGCCCACA |
NGG |
2 |
0.1882 |
Tier II |
| 5 |
NC_000012.12 |
12 |
108334837 |
- |
CMKLR1 |
NNNCTCCCTACAAGCCTACA |
NGG |
2 |
0.1719 |
Tier II |
| 6 |
NC_000009.12 |
9 |
127462505 |
- |
LRSAM1 |
NNNCTCTCTACAAGCCTCCA |
NGG |
2 |
0.0476 |
Tier II |
| 7 |
NC_000001.11 |
1 |
208672605 |
- |
LOC105372892 |
NNNCTCTCTACAAGCTGATA |
NGG |
2 |
0.142 |
Tier III |
| 8 |
NC_000012.12 |
12 |
102205057 |
- |
LOC124902998 |
NNNCTCTCTACAAGCCCACA |
NGC |
2 |
0.0052 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
40394381 |
- |
Cdk19 |
NNNCTCTCTACAAGCCGACA |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000069.6 |
3 |
37365101 |
- |
Fgf2 |
NNNCTCTCAACAAGCCAACA |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000084.6 |
18 |
53311974 |
+ |
Snx24 |
NNNCACTCTACAAGCAGACA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000072.6 |
6 |
113839486 |
+ |
Atp2b2 |
NNNCTCTCTATAAGCCGACA |
NGG |
1 |
0.3077 |
Tier II |
| 5 |
NC_000069.6 |
3 |
132136714 |
+ |
Dkk2 |
NNNCTCTCTGCAAGCTGACA |
NGG |
2 |
0.1709 |
Tier II |
| 6 |
NC_000079.6 |
13 |
93435019 |
+ |
Jmy |
NNNGTCTCTACAAGCTGACA |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000071.6 |
5 |
128253167 |
- |
Tmem132d |
NNNATCTCTACAAGCCGACA |
NGC |
2 |
0.0187 |
Tier II |
| 8 |
NC_000074.6 |
8 |
32637670 |
- |
Nrg1 |
NNNCTCTCTATAAGGCGACA |
NGG |
2 |
0.0154 |
Tier II |
| 9 |
NC_000083.6 |
17 |
36027493 |
- |
A930015D03Rik |
NNNCTCTCTAAAAGCAGACA |
NGG |
2 |
0.75 |
Tier III |
| 10 |
NC_000084.6 |
18 |
53311974 |
+ |
LOC115488927 |
NNNCACTCTACAAGCAGACA |
NGG |
2 |
0.5 |
Tier III |
| 11 |
NC_000076.6 |
10 |
110808004 |
- |
1700020G17Rik |
NNNCTCTCTACAAGACAACA |
NGG |
2 |
0.2074 |
Tier III |
Other clones with same target sequence:
(none)
