Construct: sgRNA BRDN0001145274
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTATCACGGAGTACTGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK11 (79858)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75561
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
131080584 |
+ |
NEK11 |
NNNTATCACGGAGTACTGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
22636588 |
- |
ZNF492 |
NNNTATCACGGAATACTGTG |
NGG |
1 |
0.9231 |
Tier II |
| 3 |
NC_000019.10 |
19 |
22838677 |
- |
ZNF723 |
NNNTATCATGGAATACTGTG |
NGG |
2 |
0.8077 |
Tier II |
| 4 |
NC_000009.12 |
9 |
129129434 |
+ |
PTPA |
NNNTATCATGGAGTACTGTG |
NAG |
2 |
0.2269 |
Tier II |
| 5 |
NC_000010.11 |
10 |
52462394 |
+ |
LNCAROD |
NNNTATCATGGGGTACTGTG |
NGG |
2 |
0.6319 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
105347978 |
- |
Nek11 |
NNNTATCACGGAATACTGTG |
NGG |
1 |
0.9231 |
Tier I |
| 2 |
NC_000067.6 |
1 |
75391422 |
- |
Speg |
NNNTATCACGCAGTACTGTA |
NGG |
2 |
0.4018 |
Tier II |
| 3 |
NC_000079.6 |
13 |
119734250 |
+ |
Nim1k |
NNNTATCACGATGTACTGTG |
NGG |
2 |
0.3333 |
Tier II |
| 4 |
NC_000079.6 |
13 |
108116413 |
- |
Ndufaf2 |
NNNTATCATGGAGTACTGTG |
NGT |
2 |
0.0141 |
Tier II |
| 5 |
NC_000069.6 |
3 |
17498671 |
- |
Gm30340 |
NNNTATCACAGAGTACTGTT |
NGG |
2 |
0.6533 |
Tier III |
Other clones with same target sequence:
(none)
