Construct: sgRNA BRDN0001145276
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTCTGGACACGCATAATGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRSK1 (84446)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77784
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
55294222 |
- |
BRSK1 |
NNNTGGACACGCATAATGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
52928377 |
- |
KRT8 |
NNNTGGACATGCATAATGGT |
NGG |
2 |
0.6588 |
Tier II |
| 3 |
NC_000008.11 |
8 |
94982667 |
- |
NDUFAF6 |
NNNTGTTCACGCATAATGGG |
NGG |
2 |
0.2917 |
Tier II |
| 4 |
NC_000016.10 |
16 |
28845176 |
- |
TUFM |
NNNTGGCCACGCAGAATGGG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
4698927 |
- |
Brsk1 |
NNNTGGACATGCGTAATGGG |
NGG |
2 |
0.6138 |
Tier I |
| 2 |
NC_000076.6 |
10 |
63396988 |
- |
Dnajc12 |
NNNTGGACACACATAATGGA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000082.6 |
16 |
21619585 |
+ |
Vps8 |
NNNTAGACATGCATAATGGG |
NGG |
2 |
0.8157 |
Tier II |
| 4 |
NC_000080.6 |
14 |
54829678 |
+ |
Rnf212b |
NNNGGGACACACATAATGGG |
NGG |
2 |
0.625 |
Tier II |
| 5 |
NC_000075.6 |
9 |
102516031 |
- |
Ky |
NNNGGGACACGCATGATGGG |
NGG |
2 |
0.4063 |
Tier II |
| 6 |
NC_000068.7 |
2 |
27134133 |
+ |
Fam163b |
NNNTGGACACCCATAATGAG |
NGG |
2 |
0.3061 |
Tier II |
| 7 |
NC_000071.6 |
5 |
146246583 |
- |
Cdk8 |
NNNTGGACACACATAATGGG |
NGC |
2 |
0.0222 |
Tier II |
Other clones with same target sequence:
(none)
