Construct: sgRNA BRDN0001145277
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTGTTCGAGACACAGGAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PSKH1 (5681)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000016.10 | 16 | 67909245 | + | PSKH1 | NNNTGTTCGAGACACAGGAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 167901699 | - | ADCY10 | NNNTGTTTGAGACCCAGGAG | NGG | 2 | 0.1875 | Tier I |
3 | NC_000002.12 | 2 | 71637238 | + | DYSF | NNNTGTTGGTGACACAGGAG | NGG | 2 | 0.5672 | Tier II |
4 | NC_000001.11 | 1 | 66359578 | + | PDE4B | NNNTGTTCCAGACACAGTAG | NGG | 2 | 0.359 | Tier II |
5 | NC_000001.11 | 1 | 167901699 | - | DCAF6 | NNNTGTTTGAGACCCAGGAG | NGG | 2 | 0.1875 | Tier II |
6 | NC_000010.11 | 10 | 129701338 | + | MGMT | NNNTGTTCGGGTCACAGGAG | NGG | 2 | 0.1852 | Tier II |
7 | NC_000004.12 | 4 | 3222163 | - | HTT | NNNTGTCCGAGACACATGAG | NGG | 2 | 0.1719 | Tier II |
8 | NC_000010.11 | 10 | 71544552 | + | CDH23 | NNNTGTTCCAGCCACAGGAG | NGG | 2 | 0.1417 | Tier II |
9 | NC_000010.11 | 10 | 131200392 | - | TCERG1L | NNNTGTTCCAGACACAGGAG | NAG | 2 | 0.1396 | Tier II |
10 | NC_000007.14 | 7 | 100566303 | - | AGFG2 | NNNTGTTCCAGACCCAGGAG | NGG | 2 | 0.1154 | Tier II |
11 | NC_000004.12 | 4 | 3248449 | - | MSANTD1 | NNNTGTTTGAGACACAGGAG | NGA | 2 | 0.0608 | Tier II |
12 | NC_000016.10 | 16 | 11928204 | + | NPIPB2 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
13 | NC_000016.10 | 16 | 14725559 | - | NPIPA3 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
14 | NC_000016.10 | 16 | 14725559 | - | LOC100652777 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
15 | NC_000016.10 | 16 | 14764679 | - | NPIPA2 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
16 | NC_000016.10 | 16 | 14951291 | - | NPIPA1 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
17 | NC_000016.10 | 16 | 15105080 | + | PDXDC1 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
18 | NC_000016.10 | 16 | 15364394 | + | NPIPA5 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
19 | NC_000016.10 | 16 | 16349768 | - | NPIPA6 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
20 | NC_000016.10 | 16 | 16393132 | - | NPIPA7 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
21 | NC_000016.10 | 16 | 18318760 | + | NPIPA8 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
22 | NC_000016.10 | 16 | 18358913 | + | NPIPA9 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
23 | NC_000016.10 | 16 | 21405618 | + | NPIPB3 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
24 | NC_000016.10 | 16 | 21838069 | + | NPIPB4 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
25 | NC_000016.10 | 16 | 22533302 | - | NPIPB5 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
26 | NC_000016.10 | 16 | 28343513 | + | NPIPB6 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
27 | NC_000016.10 | 16 | 28457351 | + | NPIPB7 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
28 | NC_000016.10 | 16 | 28657704 | - | NPIPB8 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
29 | NC_000016.10 | 16 | 28771829 | - | NPIPB9 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
30 | NC_000016.10 | 16 | 29384614 | + | NPIPB11 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
31 | NC_000016.10 | 16 | 29486158 | + | NPIPB12 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
32 | NC_000016.10 | 16 | 30226128 | + | NPIPB13 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
33 | NC_000016.10 | 16 | 74391067 | - | NPIPB15 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier II |
34 | NC_000004.12 | 4 | 24064001 | + | PPARGC1A | NNNTGTTCGAGACTCTGGAG | NGG | 2 | 0.0 | Tier II |
35 | NC_000013.11 | 13 | 98524418 | + | STK24 | NNNTGTTCTAGACACTGGAG | NGG | 2 | 0.0 | Tier II |
36 | NC_000008.11 | 8 | 90744661 | - | LOC105375633 | NNNTGTTCAAGACACAGGAA | NGG | 2 | 0.6027 | Tier III |
37 | NC_000014.9 | 14 | 106170741 | - | IGH | NNNTGTGAGAGACACAGGAG | NGG | 2 | 0.3824 | Tier III |
38 | NC_000014.9 | 14 | 106170741 | - | IGHVIII-16-1 | NNNTGTGAGAGACACAGGAG | NGG | 2 | 0.3824 | Tier III |
39 | NC_000016.10 | 16 | 14951291 | - | PKD1P3-NPIPA1 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
40 | NC_000016.10 | 16 | 15105080 | + | LOC100505915 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
41 | NC_000016.10 | 16 | 15105080 | + | NPIPP1 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
42 | NC_000016.10 | 16 | 15105080 | + | PKD1P6-NPIPP1 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
43 | NC_000016.10 | 16 | 16349768 | - | LOC131696449 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
44 | NC_000016.10 | 16 | 18318760 | + | PKD1P4-NPIPA8 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
45 | NC_000016.10 | 16 | 18358913 | + | PKD1P5-LOC105376752 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
46 | NC_000016.10 | 16 | 21838069 | + | LOC112268174 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
47 | NC_000016.10 | 16 | 22533302 | - | LOC105371131 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
48 | NC_000016.10 | 16 | 29051748 | - | NPIPB10P | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
49 | NC_000016.10 | 16 | 30226128 | + | LOC101929894 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
50 | NC_000016.10 | 16 | 69977057 | + | PDXDC2P-NPIPB14P | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
51 | NC_000016.10 | 16 | 69977057 | + | NPIPB14P | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
52 | NC_000018.10 | 18 | 11620245 | + | NPIPB1P | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000074.6 | 8 | 105913185 | + | Pskh1 | NNNTGTTTGAGACACAGGAG | NGT | 2 | 0.0141 | Tier I |
2 | NC_000070.6 | 4 | 77907707 | + | Ptprd | NNNTGCTCAAGACACAGGAG | NGG | 2 | 0.5844 | Tier II |
3 | NC_000086.7 | X | 151074621 | + | Fgd1 | NNNTGTACAAGACACAGGAG | NGG | 2 | 0.5625 | Tier II |
4 | NC_000082.6 | 16 | 96955800 | + | Dscam | NNNTGTTCAAGACTCAGGAG | NGG | 2 | 0.3429 | Tier II |
5 | NC_000075.6 | 9 | 109837714 | + | Nme6 | NNNTGTCCGAGACACAGCAG | NGG | 2 | 0.3274 | Tier II |
6 | NC_000073.6 | 7 | 67096827 | + | Adamts17 | NNNTGTTGGACACACAGGAG | NGG | 2 | 0.2755 | Tier II |
7 | NC_000075.6 | 9 | 123200578 | + | Cdcp1 | NNNTGTTCTAGACACAGCAG | NGG | 2 | 0.254 | Tier II |
8 | NC_000073.6 | 7 | 44592540 | - | Kcnc3 | NNNTTTTGGAGACACAGGAG | NGG | 2 | 0.1929 | Tier II |
9 | NC_000068.7 | 2 | 164789000 | - | Snx21 | NNNTTTTCTAGACACAGGAG | NGG | 2 | 0.16 | Tier II |
10 | NC_000074.6 | 8 | 118235946 | + | Cdh13 | NNNTGTTCGACACACAGGAG | NTG | 2 | 0.0167 | Tier II |
11 | NC_000079.6 | 13 | 41909013 | - | Gm10790 | NNNTGTTCCAGAGACAGGAG | NGG | 2 | 0.0734 | Tier III |