Construct: sgRNA BRDN0001145277

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGTGTTCGAGACACAGGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSKH1 (5681)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76741

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 67909245 + PSKH1 NNNTGTTCGAGACACAGGAG NGG 0 1.0 Tier I
2 NC_000001.11 1 167901699 - ADCY10 NNNTGTTTGAGACCCAGGAG NGG 2 0.1875 Tier I
3 NC_000002.12 2 71637238 + DYSF NNNTGTTGGTGACACAGGAG NGG 2 0.5672 Tier II
4 NC_000001.11 1 66359578 + PDE4B NNNTGTTCCAGACACAGTAG NGG 2 0.359 Tier II
5 NC_000001.11 1 167901699 - DCAF6 NNNTGTTTGAGACCCAGGAG NGG 2 0.1875 Tier II
6 NC_000010.11 10 129701338 + MGMT NNNTGTTCGGGTCACAGGAG NGG 2 0.1852 Tier II
7 NC_000004.12 4 3222163 - HTT NNNTGTCCGAGACACATGAG NGG 2 0.1719 Tier II
8 NC_000010.11 10 71544552 + CDH23 NNNTGTTCCAGCCACAGGAG NGG 2 0.1417 Tier II
9 NC_000010.11 10 131200392 - TCERG1L NNNTGTTCCAGACACAGGAG NAG 2 0.1396 Tier II
10 NC_000007.14 7 100566303 - AGFG2 NNNTGTTCCAGACCCAGGAG NGG 2 0.1154 Tier II
11 NC_000004.12 4 3248449 - MSANTD1 NNNTGTTTGAGACACAGGAG NGA 2 0.0608 Tier II
12 NC_000016.10 16 11928204 + NPIPB2 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
13 NC_000016.10 16 14725559 - NPIPA3 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
14 NC_000016.10 16 14725559 - LOC100652777 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
15 NC_000016.10 16 14764679 - NPIPA2 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
16 NC_000016.10 16 14951291 - NPIPA1 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
17 NC_000016.10 16 15105080 + PDXDC1 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
18 NC_000016.10 16 15364394 + NPIPA5 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
19 NC_000016.10 16 16349768 - NPIPA6 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
20 NC_000016.10 16 16393132 - NPIPA7 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
21 NC_000016.10 16 18318760 + NPIPA8 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
22 NC_000016.10 16 18358913 + NPIPA9 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
23 NC_000016.10 16 21405618 + NPIPB3 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
24 NC_000016.10 16 21838069 + NPIPB4 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
25 NC_000016.10 16 22533302 - NPIPB5 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
26 NC_000016.10 16 28343513 + NPIPB6 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
27 NC_000016.10 16 28457351 + NPIPB7 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
28 NC_000016.10 16 28657704 - NPIPB8 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
29 NC_000016.10 16 28771829 - NPIPB9 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
30 NC_000016.10 16 29384614 + NPIPB11 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
31 NC_000016.10 16 29486158 + NPIPB12 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
32 NC_000016.10 16 30226128 + NPIPB13 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
33 NC_000016.10 16 74391067 - NPIPB15 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier II
34 NC_000004.12 4 24064001 + PPARGC1A NNNTGTTCGAGACTCTGGAG NGG 2 0.0 Tier II
35 NC_000013.11 13 98524418 + STK24 NNNTGTTCTAGACACTGGAG NGG 2 0.0 Tier II
36 NC_000008.11 8 90744661 - LOC105375633 NNNTGTTCAAGACACAGGAA NGG 2 0.6027 Tier III
37 NC_000014.9 14 106170741 - IGH NNNTGTGAGAGACACAGGAG NGG 2 0.3824 Tier III
38 NC_000014.9 14 106170741 - IGHVIII-16-1 NNNTGTGAGAGACACAGGAG NGG 2 0.3824 Tier III
39 NC_000016.10 16 14951291 - PKD1P3-NPIPA1 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
40 NC_000016.10 16 15105080 + LOC100505915 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
41 NC_000016.10 16 15105080 + NPIPP1 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
42 NC_000016.10 16 15105080 + PKD1P6-NPIPP1 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
43 NC_000016.10 16 16349768 - LOC131696449 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
44 NC_000016.10 16 18318760 + PKD1P4-NPIPA8 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
45 NC_000016.10 16 18358913 + PKD1P5-LOC105376752 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
46 NC_000016.10 16 21838069 + LOC112268174 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
47 NC_000016.10 16 22533302 - LOC105371131 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
48 NC_000016.10 16 29051748 - NPIPB10P NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
49 NC_000016.10 16 30226128 + LOC101929894 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
50 NC_000016.10 16 69977057 + PDXDC2P-NPIPB14P NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
51 NC_000016.10 16 69977057 + NPIPB14P NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
52 NC_000018.10 18 11620245 + NPIPB1P NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 105913185 + Pskh1 NNNTGTTTGAGACACAGGAG NGT 2 0.0141 Tier I
2 NC_000070.6 4 77907707 + Ptprd NNNTGCTCAAGACACAGGAG NGG 2 0.5844 Tier II
3 NC_000086.7 X 151074621 + Fgd1 NNNTGTACAAGACACAGGAG NGG 2 0.5625 Tier II
4 NC_000082.6 16 96955800 + Dscam NNNTGTTCAAGACTCAGGAG NGG 2 0.3429 Tier II
5 NC_000075.6 9 109837714 + Nme6 NNNTGTCCGAGACACAGCAG NGG 2 0.3274 Tier II
6 NC_000073.6 7 67096827 + Adamts17 NNNTGTTGGACACACAGGAG NGG 2 0.2755 Tier II
7 NC_000075.6 9 123200578 + Cdcp1 NNNTGTTCTAGACACAGCAG NGG 2 0.254 Tier II
8 NC_000073.6 7 44592540 - Kcnc3 NNNTTTTGGAGACACAGGAG NGG 2 0.1929 Tier II
9 NC_000068.7 2 164789000 - Snx21 NNNTTTTCTAGACACAGGAG NGG 2 0.16 Tier II
10 NC_000074.6 8 118235946 + Cdh13 NNNTGTTCGACACACAGGAG NTG 2 0.0167 Tier II
11 NC_000079.6 13 41909013 - Gm10790 NNNTGTTCCAGAGACAGGAG NGG 2 0.0734 Tier III
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Other clones with same target sequence:

(none)