Construct: sgRNA BRDN0001145284
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGTACACTGTGTCATGCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SPEG (10290)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77623
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
219469369 |
- |
SPEG |
NNNTACACTGTGTCATGCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
44913450 |
+ |
TGM4 |
NNNTACACTGTGCCATGCCC |
NGG |
2 |
0.3539 |
Tier II |
| 3 |
NC_000002.12 |
2 |
238259403 |
- |
PER2 |
NNNTTCACAGTGTCATGCGC |
NGG |
2 |
0.3377 |
Tier II |
| 4 |
NC_000008.11 |
8 |
40586149 |
- |
ZMAT4 |
NNNGACACTGTGTCATGCGT |
NGG |
2 |
0.1875 |
Tier II |
| 5 |
NC_000007.14 |
7 |
134900640 |
- |
CALD1 |
NNNTACACTGTGTCATGAGT |
NGG |
2 |
0.1615 |
Tier II |
| 6 |
NC_000007.14 |
7 |
75822223 |
- |
CCL24 |
NNNTACACTGTGTCTTGAGC |
NGG |
2 |
0.1077 |
Tier II |
| 7 |
NC_000022.11 |
22 |
44546005 |
+ |
LOC105373059 |
NNNTACCCTGTGTCAGGCGC |
NGG |
2 |
0.1514 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
75407089 |
- |
Speg |
NNNTACACTGTGTCATGCGT |
NGG |
1 |
0.3 |
Tier I |
| 2 |
NC_000073.6 |
7 |
132132845 |
+ |
Cpxm2 |
NNNTACACTGTGTCATACAC |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000074.6 |
8 |
76998099 |
- |
Nr3c2 |
NNNTACACTGAGCCATGCGC |
NGG |
2 |
0.5921 |
Tier II |
| 4 |
NC_000080.6 |
14 |
122668392 |
- |
Pcca |
NNNTACACTGTGTCATGTGC |
NAG |
2 |
0.1667 |
Tier II |
| 5 |
NC_000070.6 |
4 |
8786650 |
+ |
Chd7 |
NNNTACACTGTGTCATGTGC |
NGA |
2 |
0.0446 |
Tier II |
Other clones with same target sequence:
(none)
