Construct: sgRNA BRDN0001145286
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAGCCCGGGCCGTGCAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST3 (23031)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76103
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
18123593 |
+ |
MAST3 |
NNNGCCCGGGCCGTGCAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
21396012 |
+ |
NKX2-4 |
NNNCCCCGGGCCGTGCAGCG |
NGG |
2 |
0.2118 |
Tier I |
| 3 |
NC_000022.11 |
22 |
41238186 |
+ |
CHADL |
NNNGCCCGGGCCCTGCAGCG |
NGG |
2 |
0.1684 |
Tier I |
| 4 |
NC_000011.10 |
11 |
74493433 |
+ |
LIPT2 |
NNNGCCCGGGCCGTGGAAGG |
NGG |
2 |
0.0192 |
Tier I |
| 5 |
NC_000006.12 |
6 |
7726433 |
+ |
BMP6 |
NNNGCCTGGGCCCTGCAACG |
NGG |
2 |
0.3421 |
Tier II |
| 6 |
NC_000011.10 |
11 |
74493433 |
+ |
LIPT2-AS1 |
NNNGCCCGGGCCGTGGAAGG |
NGG |
2 |
0.0192 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
18151086 |
- |
Rtn4r |
NNNGCCCAGGCCGTGGAACG |
NGG |
2 |
0.1538 |
Tier I |
| 2 |
NC_000073.6 |
7 |
100159553 |
- |
Lipt2 |
NNNGCCCGGGCCGTGGAAAG |
NGG |
2 |
0.0659 |
Tier I |
| 3 |
NC_000081.6 |
15 |
101351459 |
+ |
Krt80 |
NNNGCCCGGGCTGTGCAAGG |
NGG |
2 |
0.0673 |
Tier II |
| 4 |
NC_000069.6 |
3 |
116007398 |
+ |
Slc30a7 |
NNNGCCCGGGCCGCGCAACG |
NGT |
2 |
0.0046 |
Tier II |
| 5 |
NC_000081.6 |
15 |
79109001 |
+ |
Micall1 |
NNNGCCCGGGCCGGGCCACG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000073.6 |
7 |
100159553 |
- |
Gm51452 |
NNNGCCCGGGCCGTGGAAAG |
NGG |
2 |
0.0659 |
Tier III |
Other clones with same target sequence:
(none)
