Construct: sgRNA BRDN0001145287

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTGAGCGCACTGACCCCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK6 (56924)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76923

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 40266057 + PAK6 NNNGAGCGCACTGACCCCCA NGG 0 1.0 Tier I
2 NC_000015.10 15 40266057 + BUB1B-PAK6 NNNGAGCGCACTGACCCCCA NGG 0 1.0 Tier I
3 NC_000005.10 5 179344104 - ADAMTS2 NNNGTGCGCACTGACGCCCA NGG 2 0.0559 Tier I
4 NC_000022.11 22 30381239 - RNF215 NNNGAGCGCACAGACCCCCA NGG 1 0.8 Tier II
5 NC_000010.11 10 79430185 - ZCCHC24 NNNGAGAGCACTGACACCCA NGG 2 0.75 Tier II
6 NC_000019.10 19 4259969 - YJU2 NNNGATCACACTGACCCCCA NGG 2 0.6667 Tier II
7 NC_000012.12 12 2498660 - CACNA1C NNNGAGGGCACTGACCCCCA NGG 1 0.4706 Tier II
8 NC_000022.11 22 30405423 - SEC14L2 NNNGACCTCACTGACCCCCA NGG 2 0.4261 Tier II
9 NC_000020.11 20 63979188 + SAMD10 NNNGGGCGCGCTGACCCCCA NGG 2 0.4 Tier II
10 NC_000017.11 17 46814762 + WNT3 NNNCAGCCCACTGACCCCCA NGG 2 0.3258 Tier II
11 NC_000017.11 17 46814762 + LRRC37A2 NNNCAGCCCACTGACCCCCA NGG 2 0.3258 Tier II
12 NC_000009.12 9 137164808 - GRIN1 NNNGAGGCCACTGACCCCCA NGG 2 0.2896 Tier II
13 NC_000019.10 19 54277846 - LILRB2 NNNGAGCGCAGCGACCCCCA NGG 2 0.2368 Tier II
14 NC_000012.12 12 131830872 + MMP17 NNNGAGCGCACTGTCCCCCC NGG 2 0.1212 Tier II
15 NC_000023.11 X 103791207 + PLP1 NNNGAGAGCACTGACCCCGA NGG 2 0.0938 Tier II
16 NC_000023.11 X 103791207 + RAB9B NNNGAGAGCACTGACCCCGA NGG 2 0.0938 Tier II
17 NC_000003.12 3 49676263 - APEH NNNGAGCACACTGAGCCCCA NGG 2 0.05 Tier II
18 NC_000018.10 18 5878360 - MIR3976HG NNNGAACGCAATGACCCCCA NGG 2 0.75 Tier III
19 NC_000017.11 17 81864597 - LOC124904086 NNNGAGGGCTCTGACCCCCA NGG 2 0.4152 Tier III
20 NC_000015.10 15 93934006 + LINC01581 NNNGTCCGCACTGACCCCCA NGG 2 0.2479 Tier III
21 NC_000015.10 15 93934006 + LINC01580 NNNGTCCGCACTGACCCCCA NGG 2 0.2479 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 100878866 + Gpr68 NNNGAGCACACTGACACCCA NGG 2 1.0 Tier I
2 NC_000077.6 11 87611953 - Mtmr4 NNNGAGCCCACTGGCCCCCA NGG 2 0.2872 Tier I
3 NC_000068.7 2 118689949 + Pak6 NNNGAGCACACTGATCCCCA NGG 2 0.0667 Tier I
4 NC_000077.6 11 50603295 + Adamts2 NNNGTGCGCACTGACGCCCA NGG 2 0.0559 Tier I
5 NC_000079.6 13 109199294 - Pde4d NNNGAGAGAACTGACCCCCA NGG 2 0.6429 Tier II
6 NC_000071.6 5 150163648 + Fry NNNAAGCTCACTGACCCCCA NGG 2 0.5625 Tier II
7 NC_000085.6 19 28302929 - Glis3 NNNGACAGCACTGACCCCCA NGG 2 0.5114 Tier II
8 NC_000073.6 7 139924592 - Kndc1 NNNGACCTCACTGACCCCCA NGG 2 0.4261 Tier II
9 NC_000086.7 X 101429931 - Nono NNNGAGCGCTCTGGCCCCCA NGG 2 0.4118 Tier II
10 NC_000069.6 3 130347333 + Col25a1 NNNGAGCTCACTGTCCCCCA NGG 2 0.3333 Tier II
11 NC_000077.6 11 103778008 - Wnt3 NNNCAGCCCACTGACCCCCA NGG 2 0.3258 Tier II
12 NC_000081.6 15 27922777 + Trio NNNGAGCTCACTGACCACCA NGG 2 0.2917 Tier II
13 NC_000077.6 11 74906723 + Srr NNNGAGTGCCCTGACCCCCA NGG 2 0.2708 Tier II
14 NC_000077.6 11 74906723 + Tsr1 NNNGAGTGCCCTGACCCCCA NGG 2 0.2708 Tier II
15 NC_000081.6 15 80094371 + Syngr1 NNNGAGCCCACTGACCCCAA NGG 2 0.2637 Tier II
16 NC_000067.6 1 132366593 - Tmcc2 NNNGAGAGCATTGACCCCCA NGG 2 0.2308 Tier II
17 NC_000083.6 17 85615114 - Six3 NNNTAGCGCACTGACCCCGA NGG 2 0.0455 Tier II
18 NC_000074.6 8 120594798 - Gm10614 NNNGAGGGCACTGACCCCCG NGG 2 0.3599 Tier III
19 NC_000086.7 X 81504005 - LOC115489112 NNNGAGGTCACTGACCCCCA NGG 2 0.2941 Tier III
20 NC_000083.6 17 79448250 - Gm32954 NNNGAGTGCATTGACCCCCA NGG 2 0.25 Tier III
21 NC_000083.6 17 85615114 - Six3os1 NNNTAGCGCACTGACCCCGA NGG 2 0.0455 Tier III
22 NC_000084.6 18 20618301 + Gm16090 NNNCAGCGCACTGAGCCCCA NGG 2 0.0265 Tier III
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Other clones with same target sequence:

(none)