Construct: sgRNA BRDN0001145295

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATCCAGGGCTACAATGTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK1 (8536)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77477

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 9763199 + CAMK1 NNNCCAGGGCTACAATGTTG NGG 0 1.0 Tier I
2 NC_000023.11 X 129829060 - ZDHHC9 NNNCCATGGCTACACTGTTG NGG 2 0.1558 Tier I
3 NC_000021.9 21 46272595 - MCM3AP NNNCCAGGGCTACAATGTTC NGC 2 0.0095 Tier I
4 NC_000003.12 3 9763199 + OGG1 NNNCCAGGGCTACAATGTTG NGG 0 1.0 Tier II
5 NC_000004.12 4 4432664 + STX18 NNNCCAGGGCTGCAATGATG NGG 2 0.4815 Tier II
6 NC_000021.9 21 25976795 + APP NNNCCAGTGCAACAATGTTG NGG 2 0.4688 Tier II
7 NC_000022.11 22 50054482 + TTLL8 NNNCCAGGGCCATAATGTTG NGG 2 0.4667 Tier II
8 NC_000012.12 12 121422986 + RNF34 NNNCCAGGGCTACAAAGCTG NGG 2 0.3896 Tier II
9 NC_000012.12 12 121422986 + KDM2B NNNCCAGGGCTACAAAGCTG NGG 2 0.3896 Tier II
10 NC_000005.10 5 7435947 - ADCY2 NNNCCAAGGCTAAAATGTTG NGG 2 0.3846 Tier II
11 NC_000021.9 21 15781045 + USP25 NNNCCTGGGCTACAAGGTTG NGG 2 0.2473 Tier II
12 NC_000004.12 4 141695059 - IL15 NNNCCAGGGCTACCATATTG NGG 2 0.2 Tier II
13 NC_000015.10 15 42190514 - VPS39 NNNGCAGGGCTACACTGTTG NGG 2 0.1364 Tier II
14 NC_000010.11 10 113030226 + TCF7L2 NNNCAAGGGCTACCATGTTG NGG 2 0.1224 Tier II
15 NC_000005.10 5 139482342 - STING1 NNNCCAGGGCTAGAGTGTTG NGG 2 0.0886 Tier II
16 NC_000019.10 19 29667678 + PLEKHF1 NNNCCAGGGCTACCATCTTG NGG 2 0.0504 Tier II
17 NC_000020.11 20 1513464 - LOC105372499 NNNCCTGGGCTCCAATGTTG NGG 2 0.188 Tier III
18 NT_187505.1 34129 + LOC105379562 NNNCCAGGGCTACAATGCTG NCG 2 0.0459 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 113339567 + Camk1 NNNCCAGGGCTACAATGTTG NGG 0 1.0 Tier I
2 NC_000070.6 4 152315966 + Rnf207 NNNCCAGGGCTACAATGTCA NGG 2 0.2586 Tier I
3 NC_000082.6 16 45560296 - Slc9c1 NNNCCATGGCTTCAATGTTG NGG 2 0.1905 Tier I
4 NC_000083.6 17 36845128 + Trim26 NNNCTAGGGCTACAATATTG NGG 2 0.5939 Tier II
5 NC_000076.6 10 30742537 + Ncoa7 NNNTAAGGGCTACAATGTTG NGG 2 0.4571 Tier II
6 NC_000074.6 8 27116583 - Adgra2 NNNCCAGGGCTATAGTGTTG NGG 2 0.455 Tier II
7 NC_000082.6 16 17872763 - Dgcr2 NNNCCAGGGCTACGATGATG NGG 2 0.3111 Tier II
8 NC_000071.6 5 27423776 + Dpp6 NNNCCAGGGCTACAGTGTTC NGG 2 0.2786 Tier II
9 NC_000077.6 11 16791787 - Egfr NNNCCAGTGCGACAATGTTG NGG 2 0.25 Tier II
10 NC_000082.6 16 13662122 + Parn NNNCCAGGGCTACAAAGTTG NAG 2 0.2357 Tier II
11 NC_000071.6 5 31322335 - Gckr NNNCCAGGGCTACAAACTTG NGG 2 0.2139 Tier II
12 NC_000077.6 11 29932188 - Eml6 NNNCCTGGGCTACACTGTTG NGG 2 0.1948 Tier II
13 NC_000068.7 2 33568504 - Lmx1b NNNCCAGGGCTCCAATGTTT NGG 2 0.1842 Tier II
14 NC_000081.6 15 84953852 - 5031439G07Rik NNNCCAGGGCTACACTGATG NGG 2 0.1818 Tier II
15 NC_000070.6 4 106966200 - Ssbp3 NNNCCAGGGCTACATAGTTG NGG 2 0.1818 Tier II
16 NC_000071.6 5 65887528 + Rhoh NNNCCAGGGCTACAAGTTTG NGG 2 0.0865 Tier II
17 NC_000081.6 15 35614600 - Vps13b NNNCCAGGGCTACAATCGTG NGG 2 0.0784 Tier II
18 NC_000080.6 14 22584598 + Lrmda NNNCCAGGGCTAGAATGCTG NGG 2 0.0584 Tier II
19 NC_000070.6 4 87967003 + Mllt3 NNNCCAGGACTACAATGTTG NGA 2 0.0446 Tier II
20 NC_000067.6 1 182503212 + Capn2 NNNCCATGGCTACAATGTTG NTG 2 0.0223 Tier II
21 NC_000072.6 6 122627205 - Dppa3 NNNCCAGGGCTACAAGGTTG NGT 2 0.0056 Tier II
22 NC_000083.6 17 67915993 - Arhgap28 NNNCCAGGGCTACAAGGTTG NGT 2 0.0056 Tier II
23 NC_000072.6 6 48253914 - Gm16630 NNNCCAAGGCTACAATGTTC NGG 2 0.4286 Tier III
24 NC_000077.6 11 16791787 - Gm39584 NNNCCAGTGCGACAATGTTG NGG 2 0.25 Tier III
25 NC_000067.6 1 76071519 - Gm32622 NNNCCAGGGCTCCAATGTTC NGG 2 0.1128 Tier III
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Other clones with same target sequence:

(none)