Construct: sgRNA BRDN0001145303
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTCATCATCATTCAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABL1 (25)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77733
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
130862824 |
+ |
ABL1 |
NNNTCATCATCATTCAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
82146213 |
- |
CCDC57 |
NNNTCATCATTATTCAACGT |
NGG |
2 |
0.2154 |
Tier II |
| 3 |
NC_000016.10 |
16 |
81885884 |
- |
PLCG2 |
NNNTCATCATCATTCAACCC |
NGG |
2 |
0.1921 |
Tier II |
| 4 |
NC_000023.11 |
X |
29818224 |
+ |
IL1RAPL1 |
NNNTCATCATCATTGAACAG |
NGG |
2 |
0.0357 |
Tier II |
| 5 |
NC_000010.11 |
10 |
120530113 |
- |
PLPP4 |
NNNTCATCATCATTGAATGG |
NGG |
2 |
0.0321 |
Tier II |
| 6 |
NC_000002.12 |
2 |
209716357 |
- |
MAP2 |
NNNTCATAATCATTCAACGG |
NGT |
2 |
0.0105 |
Tier II |
| 7 |
NC_000009.12 |
9 |
71431760 |
- |
TRPM3 |
NNNTCATCATCATTCCACTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000023.11 |
X |
129316353 |
+ |
LOC107985684 |
NNNTCATCATCTTTCAAAGG |
NGG |
2 |
0.1795 |
Tier III |
| 9 |
NC_000016.10 |
16 |
72294670 |
+ |
LINC01572 |
NNNTCATCATCCTTCAAAGG |
NGG |
2 |
0.1417 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
63968903 |
+ |
Cyrib |
NNNTCATCATAATTCAACTG |
NGG |
2 |
0.5 |
Tier II |
| 2 |
NC_000068.7 |
2 |
77648931 |
- |
Zfp385b |
NNNTCCTCAGCATTCAACGG |
NGG |
2 |
0.2273 |
Tier II |
| 3 |
NC_000081.6 |
15 |
98203292 |
- |
Olfr287 |
NNNTCATCTTCTTTCAACGG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000072.6 |
6 |
53684053 |
+ |
Creb5 |
NNNTCATCATCATTCAACTG |
NCG |
2 |
0.0714 |
Tier II |
Other clones with same target sequence:
(none)
