Construct: sgRNA BRDN0001145304
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGACCTGGTGGTCTACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SBK1 (388228)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75797
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000016.10 |
16 |
28317607 |
+ |
SBK1 |
NNNGACCTGGTGGTCTACAA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000012.12 |
12 |
6550714 |
- |
IFFO1 |
NNNGAGCTGGTGGTCTTCAA |
NGG |
2 |
0.0667 |
Tier I |
3 |
NC_000019.10 |
19 |
47752597 |
- |
NOP53 |
NNNTACCTGGTGGTCTTCAA |
NGG |
2 |
0.0485 |
Tier I |
4 |
NC_000004.12 |
4 |
21642832 |
+ |
KCNIP4 |
NNNGACCTGGTACTCTACAA |
NGG |
2 |
0.393 |
Tier II |
5 |
NC_000012.12 |
12 |
122945253 |
- |
ABCB9 |
NNNGAGCTGGTGGTCTACAG |
NGG |
2 |
0.3824 |
Tier II |
6 |
NC_000003.12 |
3 |
133425274 |
+ |
BFSP2 |
NNNGACCTGGAGGTCTAGAA |
NGG |
2 |
0.1 |
Tier II |
7 |
NC_000003.12 |
3 |
50276134 |
- |
SEMA3B |
NNNGAGCTGGTGGTCTTCAA |
NGG |
2 |
0.0667 |
Tier II |
8 |
NC_000016.10 |
16 |
65106320 |
+ |
CDH11 |
NNNGACCTGGTGGTCTACAG |
NTG |
2 |
0.0298 |
Tier II |
9 |
NC_000009.12 |
9 |
4044656 |
+ |
GLIS3 |
NNNGACCTGGTGGTCTACAA |
NGT |
1 |
0.0161 |
Tier II |
10 |
NC_000011.10 |
11 |
89532791 |
+ |
NOX4 |
NNNGACCTGGTGGGCTACAA |
NAG |
2 |
0.0 |
Tier II |
11 |
NC_000012.12 |
12 |
9378923 |
+ |
LOC101928030 |
NNNGAGCTGGTGGTCTAAAA |
NGG |
2 |
0.2692 |
Tier III |
12 |
NC_000012.12 |
12 |
9378923 |
+ |
LINC02367 |
NNNGAGCTGGTGGTCTAAAA |
NGG |
2 |
0.2692 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000068.7 |
2 |
28229188 |
+ |
Olfm1 |
NNNGGCCAGGTGGTCTACAA |
NGG |
2 |
0.576 |
Tier I |
2 |
NC_000085.6 |
19 |
46514450 |
- |
Trim8 |
NNNGACTTGGTGTTCTACAA |
NGG |
2 |
0.2438 |
Tier I |
3 |
NC_000080.6 |
14 |
39472487 |
- |
Nrg3 |
NNNGACCTGGTGGACTCCAA |
NGG |
2 |
0.1092 |
Tier I |
4 |
NC_000072.6 |
6 |
125149818 |
+ |
Iffo1 |
NNNGAGCTGGTGGTCTTCAA |
NGG |
2 |
0.0667 |
Tier I |
5 |
NC_000073.6 |
7 |
126290079 |
+ |
Sbk1 |
NNNGACCTGGTGGCTTACAA |
NGG |
2 |
0.019 |
Tier I |
6 |
NC_000084.6 |
18 |
62517130 |
- |
Fbxo38 |
NNNGACCTAGTGGTCTACAA |
NGC |
2 |
0.0143 |
Tier I |
7 |
NC_000080.6 |
14 |
67818691 |
- |
Dock5 |
NNNGACCTGGTGGTGTACAA |
NGT |
2 |
0.0008 |
Tier I |
8 |
NC_000068.7 |
2 |
31020945 |
- |
Usp20 |
NNNGACCTGGTGGTCTAAAG |
NGG |
2 |
0.4118 |
Tier II |
9 |
NC_000080.6 |
14 |
11735103 |
- |
Ptprg |
NNNCACCTGGAGGTCTACAA |
NGG |
2 |
0.3971 |
Tier II |
10 |
NC_000074.6 |
8 |
118618504 |
- |
Cdh13 |
NNNGCCCTGCTGGTCTACAA |
NGG |
2 |
0.2 |
Tier II |
11 |
NC_000069.6 |
3 |
103600772 |
- |
Syt6 |
NNNTACCTGGTGGTCTAAAA |
NGG |
2 |
0.1958 |
Tier II |
12 |
NC_000071.6 |
5 |
125311426 |
+ |
Scarb1 |
NNNAACCTGGTGGTCTACCA |
NGG |
2 |
0.1862 |
Tier II |
13 |
NC_000070.6 |
4 |
134604011 |
- |
Man1c1 |
NNNGACCTGGTGGCCTACAT |
NGG |
2 |
0.1714 |
Tier II |
14 |
NC_000076.6 |
10 |
86128570 |
- |
Syn3 |
NNNGACCTGGTGGTCAAGAA |
NGG |
2 |
0.1212 |
Tier II |
15 |
NC_000068.7 |
2 |
49834269 |
+ |
Lypd6b |
NNNGACCTGGTGGTTCACAA |
NGG |
2 |
0.0444 |
Tier II |
16 |
NC_000073.6 |
7 |
18528609 |
+ |
Psg25 |
NNNGACCTGCTGGTCTACAA |
NGA |
2 |
0.0278 |
Tier II |
17 |
NC_000083.6 |
17 |
12265735 |
- |
Map3k4 |
NNNGACCTGGTGGTTTACCA |
NGG |
2 |
0.0138 |
Tier II |
18 |
NC_000070.6 |
4 |
151744634 |
- |
Camta1 |
NNNGACCTGGTGGTCTACAT |
NGT |
2 |
0.0097 |
Tier II |
19 |
NC_000085.6 |
19 |
3644439 |
- |
Lrp5 |
NNNCACCTGGTGGTCTACAA |
NGT |
2 |
0.0085 |
Tier II |
20 |
NC_000071.6 |
5 |
125311426 |
+ |
Gm32701 |
NNNAACCTGGTGGTCTACCA |
NGG |
2 |
0.1862 |
Tier III |
21 |
NC_000073.6 |
7 |
118056519 |
+ |
Gm34878 |
NNNCACCTGGTGGCCTACAA |
NGG |
2 |
0.1513 |
Tier III |
22 |
NC_000080.6 |
14 |
39472487 |
- |
LOC432842 |
NNNGACCTGGTGGACTCCAA |
NGG |
2 |
0.1092 |
Tier III |
23 |
NC_000073.6 |
7 |
78705554 |
+ |
E430016F16Rik |
NNNGACCTGGTGTTCTACCA |
NGG |
2 |
0.0621 |
Tier III |
24 |
NC_000083.6 |
17 |
12265735 |
- |
Gm46605 |
NNNGACCTGGTGGTTTACCA |
NGG |
2 |
0.0138 |
Tier III |
25 |
NC_000070.6 |
4 |
151744634 |
- |
Gm52710 |
NNNGACCTGGTGGTCTACAT |
NGT |
2 |
0.0097 |
Tier III |
Other clones with same target sequence:
(none)