Construct: sgRNA BRDN0001145304

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTGACCTGGTGGTCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SBK1 (388228)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75797

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 28317607 + SBK1 NNNGACCTGGTGGTCTACAA NGG 0 1.0 Tier I
2 NC_000012.12 12 6550714 - IFFO1 NNNGAGCTGGTGGTCTTCAA NGG 2 0.0667 Tier I
3 NC_000019.10 19 47752597 - NOP53 NNNTACCTGGTGGTCTTCAA NGG 2 0.0485 Tier I
4 NC_000004.12 4 21642832 + KCNIP4 NNNGACCTGGTACTCTACAA NGG 2 0.393 Tier II
5 NC_000012.12 12 122945253 - ABCB9 NNNGAGCTGGTGGTCTACAG NGG 2 0.3824 Tier II
6 NC_000003.12 3 133425274 + BFSP2 NNNGACCTGGAGGTCTAGAA NGG 2 0.1 Tier II
7 NC_000003.12 3 50276134 - SEMA3B NNNGAGCTGGTGGTCTTCAA NGG 2 0.0667 Tier II
8 NC_000016.10 16 65106320 + CDH11 NNNGACCTGGTGGTCTACAG NTG 2 0.0298 Tier II
9 NC_000009.12 9 4044656 + GLIS3 NNNGACCTGGTGGTCTACAA NGT 1 0.0161 Tier II
10 NC_000011.10 11 89532791 + NOX4 NNNGACCTGGTGGGCTACAA NAG 2 0.0 Tier II
11 NC_000012.12 12 9378923 + LOC101928030 NNNGAGCTGGTGGTCTAAAA NGG 2 0.2692 Tier III
12 NC_000012.12 12 9378923 + LINC02367 NNNGAGCTGGTGGTCTAAAA NGG 2 0.2692 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 28229188 + Olfm1 NNNGGCCAGGTGGTCTACAA NGG 2 0.576 Tier I
2 NC_000085.6 19 46514450 - Trim8 NNNGACTTGGTGTTCTACAA NGG 2 0.2438 Tier I
3 NC_000080.6 14 39472487 - Nrg3 NNNGACCTGGTGGACTCCAA NGG 2 0.1092 Tier I
4 NC_000072.6 6 125149818 + Iffo1 NNNGAGCTGGTGGTCTTCAA NGG 2 0.0667 Tier I
5 NC_000073.6 7 126290079 + Sbk1 NNNGACCTGGTGGCTTACAA NGG 2 0.019 Tier I
6 NC_000084.6 18 62517130 - Fbxo38 NNNGACCTAGTGGTCTACAA NGC 2 0.0143 Tier I
7 NC_000080.6 14 67818691 - Dock5 NNNGACCTGGTGGTGTACAA NGT 2 0.0008 Tier I
8 NC_000068.7 2 31020945 - Usp20 NNNGACCTGGTGGTCTAAAG NGG 2 0.4118 Tier II
9 NC_000080.6 14 11735103 - Ptprg NNNCACCTGGAGGTCTACAA NGG 2 0.3971 Tier II
10 NC_000074.6 8 118618504 - Cdh13 NNNGCCCTGCTGGTCTACAA NGG 2 0.2 Tier II
11 NC_000069.6 3 103600772 - Syt6 NNNTACCTGGTGGTCTAAAA NGG 2 0.1958 Tier II
12 NC_000071.6 5 125311426 + Scarb1 NNNAACCTGGTGGTCTACCA NGG 2 0.1862 Tier II
13 NC_000070.6 4 134604011 - Man1c1 NNNGACCTGGTGGCCTACAT NGG 2 0.1714 Tier II
14 NC_000076.6 10 86128570 - Syn3 NNNGACCTGGTGGTCAAGAA NGG 2 0.1212 Tier II
15 NC_000068.7 2 49834269 + Lypd6b NNNGACCTGGTGGTTCACAA NGG 2 0.0444 Tier II
16 NC_000073.6 7 18528609 + Psg25 NNNGACCTGCTGGTCTACAA NGA 2 0.0278 Tier II
17 NC_000083.6 17 12265735 - Map3k4 NNNGACCTGGTGGTTTACCA NGG 2 0.0138 Tier II
18 NC_000070.6 4 151744634 - Camta1 NNNGACCTGGTGGTCTACAT NGT 2 0.0097 Tier II
19 NC_000085.6 19 3644439 - Lrp5 NNNCACCTGGTGGTCTACAA NGT 2 0.0085 Tier II
20 NC_000071.6 5 125311426 + Gm32701 NNNAACCTGGTGGTCTACCA NGG 2 0.1862 Tier III
21 NC_000073.6 7 118056519 + Gm34878 NNNCACCTGGTGGCCTACAA NGG 2 0.1513 Tier III
22 NC_000080.6 14 39472487 - LOC432842 NNNGACCTGGTGGACTCCAA NGG 2 0.1092 Tier III
23 NC_000073.6 7 78705554 + E430016F16Rik NNNGACCTGGTGTTCTACCA NGG 2 0.0621 Tier III
24 NC_000083.6 17 12265735 - Gm46605 NNNGACCTGGTGGTTTACCA NGG 2 0.0138 Tier III
25 NC_000070.6 4 151744634 - Gm52710 NNNGACCTGGTGGTCTACAT NGT 2 0.0097 Tier III
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Other clones with same target sequence:

(none)