Construct: sgRNA BRDN0001145305
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATTCGCTTCAATGCACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMP2K (55589)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75927
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
78826086 |
- |
BMP2K |
NNNTTCGCTTCAATGCACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
143158861 |
- |
ARHGAP15 |
NNNTTTGCTGCAATGCACAT |
NGG |
2 |
0.4643 |
Tier II |
| 3 |
NC_000010.11 |
10 |
20919349 |
+ |
NEBL |
NNNTTCCCTTCTATGCACAT |
NGG |
2 |
0.2292 |
Tier II |
| 4 |
NC_000011.10 |
11 |
106942619 |
- |
GUCY1A2 |
NNNTTCTCTTCAATGCACAT |
NGA |
2 |
0.0397 |
Tier II |
| 5 |
NC_000018.10 |
18 |
13572563 |
+ |
LDLRAD4 |
NNNTTGGCTTCAATGCACAT |
NGA |
2 |
0.0347 |
Tier II |
| 6 |
NC_000002.12 |
2 |
132487441 |
- |
GPR39 |
NNNTTCGCTTCAATGCACTT |
NGC |
2 |
0.012 |
Tier II |
| 7 |
NC_000004.12 |
4 |
78826086 |
- |
LOC105377299 |
NNNTTCGCTTCAATGCACAT |
NGG |
0 |
1.0 |
Tier III |
| 8 |
NC_000023.11 |
X |
27283701 |
- |
LOC105373150 |
NNNTTCAATTCAATGCACAT |
NGG |
2 |
0.65 |
Tier III |
| 9 |
NC_000002.12 |
2 |
143158861 |
- |
LOC107985949 |
NNNTTTGCTGCAATGCACAT |
NGG |
2 |
0.4643 |
Tier III |
| 10 |
NC_000004.12 |
4 |
106653444 |
+ |
LOC105377356 |
NNNTTCCCTTCTATGCACAT |
NGG |
2 |
0.2292 |
Tier III |
| 11 |
NC_000009.12 |
9 |
2477885 |
- |
LOC101930053 |
NNNTTCTCTTCAATGCACAT |
NAG |
2 |
0.1481 |
Tier III |
| 12 |
NC_000014.9 |
14 |
97957837 |
+ |
LINC01550 |
NNNTTCTCTTCAATTCACAT |
NGG |
2 |
0.0816 |
Tier III |
| 13 |
NC_000023.11 |
X |
27284913 |
- |
LOC105373150 |
NNNTTCTCTTCAATGCACAT |
NTG |
2 |
0.0223 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
97027880 |
- |
Bmp2k |
NNNTTCGCTTCAGTGCACAT |
NGG |
1 |
0.6522 |
Tier I |
| 2 |
NC_000079.6 |
13 |
68864174 |
+ |
Adcy2 |
NNNTTCTCTTCATTGCACAT |
NGG |
2 |
0.1714 |
Tier II |
Other clones with same target sequence:
(none)
