Construct: sgRNA BRDN0001145307
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGCCTAGAGTTTGACAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GK (2710)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76327
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
30696050 |
+ |
GK |
NNNGCCTAGAGTTTGACAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
19368052 |
+ |
B9D1 |
NNNGCCTAGAGTCTGACATG |
NGG |
2 |
0.5263 |
Tier II |
| 3 |
NC_000002.12 |
2 |
96739596 |
- |
LMAN2L |
NNNGCCTGGAGTTAGACAGG |
NGG |
2 |
0.454 |
Tier II |
| 4 |
NC_000023.11 |
X |
155116966 |
+ |
BRCC3 |
NNNGCCTAGAGTAAGACAGG |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000013.11 |
13 |
87649002 |
+ |
LOC124900338 |
NNNGCCTTGAGTTTGACAGA |
NGG |
2 |
0.4018 |
Tier II |
| 6 |
NC_000009.12 |
9 |
95459084 |
- |
PTCH1 |
NNNGCCCAGAGTTTGAAAGG |
NGG |
2 |
0.3208 |
Tier II |
| 7 |
NC_000023.11 |
X |
125042911 |
+ |
TENM1 |
NNNGCCTAGAGTTTGACAAC |
NGG |
2 |
0.3061 |
Tier II |
| 8 |
NC_000005.10 |
5 |
134367783 |
- |
CDKL3 |
NNNTCCTAGAGTTTGACATG |
NGG |
2 |
0.2424 |
Tier II |
| 9 |
NC_000011.10 |
11 |
12425280 |
- |
PARVA |
NNNGCCTAGTGTTTCACAGG |
NGG |
2 |
0.2406 |
Tier II |
| 10 |
NC_000006.12 |
6 |
147778343 |
- |
SAMD5 |
NNNGCCTAGAGTCTGGCAGG |
NGG |
2 |
0.1518 |
Tier II |
| 11 |
NC_000003.12 |
3 |
116009080 |
- |
LSAMP |
NNNGCCTAGAGATTGAGAGG |
NGG |
2 |
0.0471 |
Tier II |
| 12 |
NC_000023.11 |
X |
118436210 |
+ |
WDR44 |
NNNGCCTAGAGTGTGACAGG |
NGC |
2 |
0.0058 |
Tier II |
| 13 |
NC_000007.14 |
7 |
96075568 |
+ |
DYNC1I1 |
NNNGCCTAGAGTTGGACTGG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000007.14 |
7 |
38725438 |
- |
VPS41 |
NNNGGCTAGAGTTTGCCAGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000017.11 |
17 |
19368052 |
+ |
LOC105371573 |
NNNGCCTAGAGTCTGACATG |
NGG |
2 |
0.5263 |
Tier III |
| 16 |
NC_000013.11 |
13 |
87649002 |
+ |
MIR4500HG |
NNNGCCTTGAGTTTGACAGA |
NGG |
2 |
0.4018 |
Tier III |
| 17 |
NC_000003.12 |
3 |
36635973 |
- |
NBPF21P |
NNNGCCTAGTGTTTTACAGG |
NGG |
2 |
0.1261 |
Tier III |
| 18 |
NC_000003.12 |
3 |
116009080 |
- |
LOC124906269 |
NNNGCCTAGAGATTGAGAGG |
NGG |
2 |
0.0471 |
Tier III |
| 19 |
NC_000005.10 |
5 |
21576244 |
- |
GUSBP1 |
NNNGCCTAGAGTTTGACATG |
NTG |
2 |
0.026 |
Tier III |
| 20 |
NC_000006.12 |
6 |
153667446 |
- |
MTCO3P31 |
NNNGCCTAGAGTTAGTCAGG |
NGG |
2 |
0.0 |
Tier III |
| 21 |
NC_000015.10 |
15 |
87352797 |
+ |
LOC102724465 |
NNNGCCTAGGGTTTGTCAGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
124214602 |
- |
Fgf14 |
NNNGCATAGTGTTTGACAGG |
NGG |
2 |
0.8193 |
Tier II |
| 2 |
NC_000077.6 |
11 |
96971413 |
- |
Sp2 |
NNNGCCTAGAATTTGACAAG |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000074.6 |
8 |
76942715 |
- |
Nr3c2 |
NNNGCCTAGTGTTTGACAGT |
NGG |
2 |
0.6176 |
Tier II |
| 4 |
NC_000082.6 |
16 |
78330738 |
- |
Cxadr |
NNNGTCTAGAGTTTGACAAG |
NGG |
2 |
0.4545 |
Tier II |
| 5 |
NC_000073.6 |
7 |
114103854 |
+ |
Rras2 |
NNNGTCTAGAGTATGACAGG |
NGG |
2 |
0.4406 |
Tier II |
| 6 |
NC_000082.6 |
16 |
70562526 |
+ |
Gbe1 |
NNNGACTGGAGTTTGACAGG |
NGG |
2 |
0.419 |
Tier II |
| 7 |
NC_000084.6 |
18 |
38620712 |
+ |
Arhgap26 |
NNNGGCTAGAGTTTGACATG |
NGG |
2 |
0.4 |
Tier II |
| 8 |
NC_000080.6 |
14 |
103123277 |
- |
Mycbp2 |
NNNGCCTAGATTTTAACAGG |
NGG |
2 |
0.362 |
Tier II |
| 9 |
NC_000083.6 |
17 |
43867130 |
+ |
Rcan2 |
NNNGACTAGAGTTTGAAAGG |
NGG |
2 |
0.2667 |
Tier II |
| 10 |
NC_000072.6 |
6 |
87189696 |
- |
Antxr1 |
NNNGCCTGGAGTGTGACAGG |
NGG |
2 |
0.1913 |
Tier II |
| 11 |
NC_000072.6 |
6 |
116178996 |
- |
Tmcc1 |
NNNGCCTAGAGTTTTACAGA |
NGG |
2 |
0.1339 |
Tier II |
| 12 |
NC_000071.6 |
5 |
65815521 |
- |
N4bp2 |
NNNGCCTAGAATTTGAGAGG |
NGG |
2 |
0.0588 |
Tier II |
| 13 |
NC_000080.6 |
14 |
60624928 |
+ |
Spata13 |
NNNGCCTAGAGTTTGAGAGG |
NGG |
1 |
0.0588 |
Tier II |
| 14 |
NC_000086.7 |
X |
64175254 |
+ |
3830417A13Rik |
NNNGCCAAGAGTTTGACAGG |
NTG |
2 |
0.0341 |
Tier II |
| 15 |
NC_000085.6 |
19 |
58004988 |
+ |
Atrnl1 |
NNNGCCTCGAGTTTGACAGG |
NGA |
2 |
0.0298 |
Tier II |
| 16 |
NC_000081.6 |
15 |
100889888 |
+ |
Scn8a |
NNNGCCCAGAGTTTGACAGG |
NTG |
2 |
0.0268 |
Tier II |
| 17 |
NC_000072.6 |
6 |
148260983 |
+ |
Tmtc1 |
NNNACCTAGAGTTTGACAGG |
NGC |
2 |
0.02 |
Tier II |
| 18 |
NC_000075.6 |
9 |
64962789 |
- |
Ints14 |
NNNCCCTAGAGTTGGACAGG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000070.6 |
4 |
120777166 |
+ |
Nfyc |
NNNGCCTAGAGTTGGAGAGG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000071.6 |
5 |
123591247 |
+ |
Clip1 |
NNNGCCTAGAGTTTGCTAGG |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000072.6 |
6 |
148260983 |
+ |
Gm52483 |
NNNACCTAGAGTTTGACAGG |
NGC |
2 |
0.02 |
Tier III |
| 22 |
NC_000076.6 |
10 |
39568058 |
- |
Gm16364 |
NNNGCCTAGAGTTTGAGAGG |
NGT |
2 |
0.0009 |
Tier III |
Other clones with same target sequence:
(none)
