Construct: sgRNA BRDN0001145311
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGTACACAAAGCCCGGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK35 (140901)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77355
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
2102918 |
+ |
STK35 |
NNNGTACACAAAGCCCGGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
52110859 |
+ |
BCL2L10 |
NNNGTACAGAGAGCCCGGAG |
NGG |
2 |
0.4024 |
Tier II |
| 3 |
NC_000020.11 |
20 |
15032013 |
+ |
MACROD2 |
NNNGTACCCAAAGTCCGGAG |
NGG |
2 |
0.3143 |
Tier II |
| 4 |
NC_000017.11 |
17 |
15320930 |
+ |
TEKT3 |
NNNGTACACAAAGCACAGAG |
NGG |
2 |
0.2074 |
Tier II |
| 5 |
NC_000016.10 |
16 |
7128602 |
+ |
RBFOX1 |
NNNGTACTCAAAGCCTGGAG |
NGG |
2 |
0.1319 |
Tier II |
| 6 |
NC_000006.12 |
6 |
6236154 |
+ |
F13A1 |
NNNGTACACAAAGCAGGGAG |
NGG |
2 |
0.0342 |
Tier II |
| 7 |
NC_000014.9 |
14 |
63663864 |
+ |
LOC105370532 |
NNNGTGCCCAAAGCCCGGAG |
NGG |
2 |
0.3061 |
Tier III |
| 8 |
NC_000013.11 |
13 |
69265580 |
- |
LINC00383 |
NNNGTACACAAAGCCAGGAG |
NAG |
2 |
0.2593 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
129801557 |
+ |
Stk35 |
NNNGGACAAAAAGCCCGGAG |
NGG |
2 |
0.5486 |
Tier I |
| 2 |
NC_000080.6 |
14 |
46385061 |
- |
Bmp4 |
NNNGTACACAAAGCCAAGAG |
NGG |
2 |
0.9333 |
Tier II |
| 3 |
NC_000068.7 |
2 |
101954546 |
+ |
Ldlrad3 |
NNNGCACACAAGGCCCGGAG |
NGG |
2 |
0.7222 |
Tier II |
| 4 |
NC_000067.6 |
1 |
192736942 |
- |
Hhat |
NNNGTACACAAGGCCAGGAG |
NGG |
2 |
0.7222 |
Tier II |
| 5 |
NC_000077.6 |
11 |
94601389 |
- |
Acsf2 |
NNNGTACACAGAGCCAGGAG |
NGG |
2 |
0.65 |
Tier II |
| 6 |
NC_000086.7 |
X |
52576426 |
+ |
Gpc3 |
NNNGTAAACAAAGCCCGGTG |
NGG |
2 |
0.4038 |
Tier II |
| 7 |
NC_000086.7 |
X |
152201097 |
- |
Iqsec2 |
NNNGAAAACAAAGCCCGGAG |
NGG |
2 |
0.375 |
Tier II |
| 8 |
NC_000069.6 |
3 |
51486422 |
- |
Rab33b |
NNNGTAAACCAAGCCCGGAG |
NGG |
2 |
0.25 |
Tier II |
| 9 |
NC_000070.6 |
4 |
123041291 |
+ |
Trit1 |
NNNGTGCACAAAGCCCGGAG |
NGT |
2 |
0.0115 |
Tier II |
| 10 |
NC_000086.7 |
X |
52576426 |
+ |
A630012P03Rik |
NNNGTAAACAAAGCCCGGTG |
NGG |
2 |
0.4038 |
Tier III |
| 11 |
NC_000076.6 |
10 |
67532527 |
+ |
Gm32255 |
NNNGTACACACAGCCAGGAG |
NGG |
2 |
0.4 |
Tier III |
| 12 |
NC_000086.7 |
X |
152201097 |
- |
Gm52430 |
NNNGAAAACAAAGCCCGGAG |
NGG |
2 |
0.375 |
Tier III |
| 13 |
NC_000073.6 |
7 |
81737393 |
+ |
Gm42400 |
NNNTTACACAAAGCCTGGAG |
NGG |
2 |
0.1119 |
Tier III |
Other clones with same target sequence:
(none)
