Construct: sgRNA BRDN0001145312
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGAGTCTAAATCTCGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K6 (5608)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76040
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
69516862 |
- |
MAP2K6 |
NNNGAGTCTAAATCTCGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
45867886 |
- |
EPG5 |
NNNAAGTCTAAATCTCTAGG |
NGG |
2 |
0.225 |
Tier II |
| 3 |
NC_000013.11 |
13 |
101759282 |
+ |
FGF14 |
NNNGAGTCTGAATCTTGAGG |
NGG |
2 |
0.1709 |
Tier II |
| 4 |
NC_000019.10 |
19 |
10290884 |
- |
ICAM5 |
NNNGAGTCTAAACCTCGAGG |
NTG |
2 |
0.0308 |
Tier II |
| 5 |
NC_000017.11 |
17 |
49545022 |
- |
NGFR-AS1 |
NNNGAGTCTGAATCTCCAGG |
NGG |
2 |
0.1307 |
Tier III |
| 6 |
NC_000008.11 |
8 |
32976803 |
- |
LOC105379362 |
NNNGAGTCTAAATCTCCAGG |
NGT |
2 |
0.0038 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
110490152 |
- |
Map2k6 |
NNNGAGTCTAAATCCCGAGG |
NGG |
1 |
0.2727 |
Tier I |
| 2 |
NC_000073.6 |
7 |
97002032 |
+ |
Nars2 |
NNNGAGTTTAAATCTCGAAG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000076.6 |
10 |
34283552 |
- |
Tspyl1 |
NNNCAGTCTTAATCTCGAGG |
NGG |
2 |
0.4671 |
Tier II |
| 4 |
NC_000079.6 |
13 |
95524635 |
+ |
F2rl1 |
NNNGAGTGTAAAACTCGAGG |
NGG |
2 |
0.4451 |
Tier II |
| 5 |
NC_000067.6 |
1 |
187917589 |
+ |
Esrrg |
NNNGAATCTAAAGCTCGAGG |
NGG |
2 |
0.2609 |
Tier II |
| 6 |
NC_000076.6 |
10 |
34283552 |
- |
Gm26564 |
NNNCAGTCTTAATCTCGAGG |
NGG |
2 |
0.4671 |
Tier III |
| 7 |
NC_000080.6 |
14 |
46656564 |
- |
D330046F09Rik |
NNNGACTCTAAATCTCCAGG |
NGG |
2 |
0.1604 |
Tier III |
Other clones with same target sequence:
(none)
