Construct: sgRNA BRDN0001145316

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGGAGACTTCCATCCAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERN2 (10595)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75845

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 23710976 + ERN2 NNNGAGACTTCCATCCAAGG NGG 0 1.0 Tier I
2 NC_000022.11 22 37641530 + SH3BP1 NNNGAGACTTCCATCCAATG NGG 1 0.6667 Tier II
3 NC_000002.12 2 54587563 - SPTBN1 NNNGGGACTTCAATCCAAGG NGG 2 0.5143 Tier II
4 NC_000007.14 7 100870803 + TRIP6 NNNGGGGCTTCCATCCAAGG NGG 2 0.5082 Tier II
5 NC_000001.11 1 157137902 + ETV3 NNNGACACTTCCATCCAAGT NGG 2 0.4773 Tier II
6 NC_000019.10 19 36047481 - THAP8 NNNGTGGCTTCCATCCAAGG NGG 2 0.2567 Tier II
7 NC_000005.10 5 137108266 - SPOCK1 NNNGAGGCTTCCTTCCAAGG NGG 2 0.2118 Tier II
8 NC_000001.11 1 205843392 - PM20D1 NNNGAGAATTCCTTCCAAGG NGG 2 0.195 Tier II
9 NC_000001.11 1 184572995 - C1orf21 NNNGAGACTTACATACAAGG NGG 2 0.1667 Tier II
10 NC_000007.14 7 130599551 - COPG2 NNNGAGAGTTCCATACAAGG NGG 2 0.1429 Tier II
11 NC_000009.12 9 69048123 - FXN NNNGAGACTCCCATCCACGG NGG 2 0.1016 Tier II
12 NC_000004.12 4 75931092 - NAAA NNNGGGACTTCCATCCTAGG NGG 2 0.096 Tier II
13 NC_000009.12 9 116250398 + PAPPA NNNGAAACTTCCATCCAAGG NTG 2 0.039 Tier II
14 NC_000015.10 15 57642576 - GCOM1 NNNGAGACTTCCATCCATGG NGA 2 0.0347 Tier II
15 NC_000015.10 15 57642576 - MYZAP NNNGAGACTTCCATCCATGG NGA 2 0.0347 Tier II
16 NC_000004.12 4 172777732 + GALNTL6 NNNGAGACTTCCATAGAAGG NGG 2 0.0342 Tier II
17 NC_000003.12 3 40146849 - MYRIP NNNCAGACTTCCATGCAAGG NGG 2 0.0265 Tier II
18 NC_000002.12 2 108976492 - RANBP2 NNNGAGACTTCCTTCCAAGG NGC 2 0.0067 Tier II
19 NC_000002.12 2 108976492 - EDAR NNNGAGACTTCCTTCCAAGG NGC 2 0.0067 Tier II
20 NC_000014.9 14 66782452 + GPHN NNNGAGACTTCCAGCCAAGA NGG 2 0.0 Tier II
21 NC_000001.11 1 116613317 - IGSF3 NNNGAGACTTCCATCCAAGG NCT 2 0.0 Tier II
22 NC_000002.12 2 54587563 - LOC124906008 NNNGGGACTTCAATCCAAGG NGG 2 0.5143 Tier III
23 NC_000006.12 6 2625966 + LINC01600 NNNGAGAATTCCATCCAAGT NGG 2 0.455 Tier III
24 NC_000019.10 19 36047481 - LOC101927572 NNNGTGGCTTCCATCCAAGG NGG 2 0.2567 Tier III
25 NC_000002.12 2 91739851 + IGSF3P2 NNNGAGACTTCCATCCAAGG NCT 2 0.0 Tier III
26 NC_000013.11 13 18306807 - IGSF3P1 NNNGAGACTTCCATCCAAGG NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 122183913 + Ern2 NNNGAGACTTCCGTCCAAGG NAG 2 0.1691 Tier I
2 NC_000070.6 4 137555123 + Hspg2 NNNGACACTTCCATCCCAGG NGG 2 0.1203 Tier I
3 NC_000068.7 2 31009434 + Usp20 NNNGGGACTTCCATCCAAGG NGG 1 0.72 Tier II
4 NC_000074.6 8 31217414 - Fut10 NNNCACACTTCCATCCAAGG NGG 2 0.361 Tier II
5 NC_000067.6 1 182478483 - Capn2 NNNGAGACTTCCATCTAAGG NGG 1 0.3077 Tier II
6 NC_000067.6 1 106360684 + Phlpp1 NNNGAGAGTTCCATCCAAGC NGG 2 0.2755 Tier II
7 NC_000076.6 10 42859418 + Scml4 NNNGAGACTGCCATCCAAGC NGG 2 0.2143 Tier II
8 NC_000085.6 19 6184634 - Majin NNNGAAACTTCCCTCCAAGG NGG 2 0.2105 Tier II
9 NC_000067.6 1 91335768 - Espnl NNNGAGACTTCCACCCCAGG NGG 2 0.0504 Tier II
10 NC_000074.6 8 24046260 + Zmat4 NNNGAGGCTTCCATGCAAGG NGG 2 0.0353 Tier II
11 NC_000070.6 4 65306864 - Pappa NNNGAGTCTTCCATTCAAGG NGG 2 0.0292 Tier II
12 NC_000084.6 18 64514285 + Nars NNNGAGACTTCCCTCCAAGG NGT 2 0.0034 Tier II
13 NC_000068.7 2 163141106 - Gm11454 NNNGAGACTTCCATCTAAAG NGG 2 0.2198 Tier III
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Other clones with same target sequence:

(none)