Construct: sgRNA BRDN0001145317

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGAGAGTGAAACAACTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YES1 (7525)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77965

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000018.10 18 745953 - YES1 NNNGAGAGTGAAACAACTAA NGG 0 1.0 Tier I
2 NC_000006.12 6 111704004 - FYN NNNGAGAGTGAAACCACCAA NGG 2 0.1169 Tier I
3 NC_000010.11 10 79974124 - SFTPD NNNGAGAATAAAACAACTAA NGG 2 0.9333 Tier II
4 NC_000001.11 1 117455003 + MAN1A2 NNNGAGAAGGAAACAACTAA NGG 2 0.619 Tier II
5 NC_000014.9 14 72500706 + RGS6 NNNGAGACTGAAACAACAAA NGG 2 0.4103 Tier II
6 NC_000008.11 8 84186646 + RALYL NNNGAGATTGAAACAATTAA NGG 2 0.2917 Tier II
7 NC_000023.11 X 129482864 - SMARCA1 NNNTAGAGTGAGACAACTAA NGG 2 0.2626 Tier II
8 NC_000010.11 10 126261307 + ADAM12 NNNGAGATTGAAACAACGAA NGG 2 0.2083 Tier II
9 NC_000015.10 15 52592795 - ATOSA NNNGAGAGTGAAATAACTAA NAG 2 0.1901 Tier II
10 NC_000022.11 22 25648173 + GRK3 NNNGAGAGCGAAACTACTAA NGG 2 0.1846 Tier II
11 NC_000012.12 12 28513672 - CCDC91 NNNGAGAGTGATACAATTAA NGG 2 0.1556 Tier II
12 NC_000008.11 8 70597222 + TRAM1 NNNTAGAGTGATACAACTAA NGG 2 0.1212 Tier II
13 NC_000007.14 7 146663355 - CNTNAP2 NNNGAAAGTGAAACAACTAA NGA 2 0.0694 Tier II
14 NC_000002.12 2 12229162 - MIR3681HG NNNGAGAGTTAAACAACTTA NGG 2 0.4375 Tier III
15 NC_000008.11 8 62231862 - LOC124901952 NNNGAGAGTGAAGCAACAAA NGG 2 0.4348 Tier III
16 NC_000010.11 10 110484446 - DUSP5-DT NNNGAGAGTGAAAAAACTAA NGG 1 0.35 Tier III
17 NC_000004.12 4 23317488 - LOC105374523 NNNGAGATTGAAACAAATAA NGG 2 0.2917 Tier III
18 NC_000004.12 4 23317488 - LOC105374524 NNNGAGATTGAAACAAATAA NGG 2 0.2917 Tier III
19 NC_000022.11 22 25648173 + YES1P1 NNNGAGAGCGAAACTACTAA NGG 2 0.1846 Tier III
20 NC_000016.10 16 63455205 + LOC105371308 NNNGAGAGTGAAACAACTAG NGC 2 0.017 Tier III
21 NC_000011.10 11 31853909 - PAX6-AS1 NNNGAGAGTGAAACAACTGA NTG 2 0.0146 Tier III
22 NC_000004.12 4 19461864 + LOC105374511 NNNGAGAGTGAAACAACTAG NGT 2 0.0123 Tier III
23 NC_000020.11 20 60772489 + LOC105372699 NNNGAGATTGAAACACCTAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 32653062 + Yes1 NNNGAAAGTGAAACTACTAA NGG 2 0.2 Tier I
2 NC_000081.6 15 66789687 - Sla NNNGAGAGTGAAACAAAGAA NGG 2 0.1556 Tier I
3 NC_000079.6 13 57839044 + Spock1 NNNGACAGAGAAACAACTAA NGG 2 0.6331 Tier II
4 NC_000077.6 11 33407563 + Ranbp17 NNNGAGAGAGAAACAACTAT NGG 2 0.5571 Tier II
5 NC_000068.7 2 18687497 + Gm46725 NNNGAGACTGAAACAACAAA NGG 2 0.4103 Tier II
6 NC_000080.6 14 14755682 - Slc4a7 NNNGAGAGTGAAGCAACCAA NGG 2 0.2795 Tier II
7 NC_000077.6 11 66251012 + Shisa6 NNNGAGAGAGAAACAACTAC NGG 2 0.211 Tier II
8 NC_000085.6 19 7696757 - Slc22a19 NNNGAAAGTGAAACTACTAA NGG 2 0.2 Tier II
9 NC_000082.6 16 94521835 + Vps26c NNNGAGAGTGAATCAACTAT NGG 2 0.18 Tier II
10 NC_000081.6 15 66789687 - Tg NNNGAGAGTGAAACAAAGAA NGG 2 0.1556 Tier II
11 NC_000076.6 10 94763892 - Cep83 NNNGAGAGTAAAACAACTAA NGA 2 0.0648 Tier II
12 NC_000078.6 12 81118179 - Smoc1 NNNGAGATTGAAACAACTAA NGA 2 0.0434 Tier II
13 NC_000070.6 4 95777317 - Fggy NNNGAGAGGGAAACAACTAA NGA 2 0.043 Tier II
14 NC_000086.7 X 103818275 - Slc16a2 NNNGAGAGTGAAACAAATAA NTG 2 0.0182 Tier II
15 NC_000080.6 14 25983805 + Duxbl1 NNNGAGAATGAAACAACTAA NGT 2 0.0161 Tier II
16 NC_000080.6 14 26123399 + Duxbl2 NNNGAGAATGAAACAACTAA NGT 2 0.0161 Tier II
17 NC_000080.6 14 26263048 + Duxbl3 NNNGAGAATGAAACAACTAA NGT 2 0.0161 Tier II
18 NC_000077.6 11 66251012 + Gm12296 NNNGAGAGAGAAACAACTAC NGG 2 0.211 Tier III
19 NC_000085.6 19 7696757 - Gm15967 NNNGAAAGTGAAACTACTAA NGG 2 0.2 Tier III
20 NC_000082.6 16 94521835 + Gm31323 NNNGAGAGTGAATCAACTAT NGG 2 0.18 Tier III
21 NC_000073.6 7 66832123 + Gm33627 NNNGAGAGTGAAACACCTAA NGC 2 0.0 Tier III
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Other clones with same target sequence:

(none)