Construct: sgRNA BRDN0001145318

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTACTATAACAACAGTACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYN (4067)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77029

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 55966733 + LYN NNNCTATAACAACAGTACCA NGG 0 1.0 Tier I
2 NC_000001.11 1 31916757 + PTP4A2 NNNCTATAAAAACAATACCA NGG 2 0.8157 Tier II
3 NC_000001.11 1 185870838 + HMCN1 NNNCTATAACAATAGAACCA NGG 2 0.6364 Tier II
4 NC_000004.12 4 139902759 - MAML3 NNNCTATAACAACAGTACCT NGG 1 0.6 Tier II
5 NC_000001.11 1 235581223 - GNG4 NNNTTACAACAACAGTACCA NGG 2 0.55 Tier II
6 NC_000002.12 2 159746016 + MARCHF7 NNNCTAAAACAACAGTACAA NGG 2 0.375 Tier II
7 NC_000001.11 1 248512751 - OR2G6 NNNCTATAACAAAAATACCA NGG 2 0.362 Tier II
8 NC_000002.12 2 1219106 - SNTG2 NNNCTATCACAACAGTACCG NGG 2 0.3277 Tier II
9 NC_000020.11 20 51518390 + NFATC2 NNNCTATCACAGCAGTACCA NGG 2 0.3095 Tier II
10 NC_000006.12 6 89755556 + MDN1 NNNCTATAACCACAGCACCA NGG 2 0.2667 Tier II
11 NC_000001.11 1 223805024 - TP53BP2 NNNCTATAACAAAAGTACCT NGG 2 0.2308 Tier II
12 NC_000004.12 4 169163557 + SH3RF1 NNNCTATAACCACAGTAACA NGG 2 0.2154 Tier II
13 NC_000003.12 3 65780450 + MAGI1 NNNCTATAACCACAGTACTA NGG 2 0.1846 Tier II
14 NC_000023.11 X 110209934 + AMMECR1 NNNCTATAACAAAAGTACTA NGG 2 0.1775 Tier II
15 NC_000004.12 4 21887194 + KCNIP4 NNNCTATAACAAAAGTACCA NAG 2 0.0997 Tier II
16 NC_000007.14 7 12628322 + SCIN NNNCTATAACAAAAGTACCA NAG 2 0.0997 Tier II
17 NC_000002.12 2 233814996 + MROH2A NNNATATAACAACAGTACCA NGA 2 0.0585 Tier II
18 NC_000022.11 22 46612312 - GRAMD4 NNNCAATAACAACAGTACCA NCG 2 0.0536 Tier II
19 NC_000004.12 4 86450668 + MAPK10 NNNCTATAACAACATTTCCA NGG 2 0.019 Tier II
20 NC_000001.11 1 248512751 - LOC105373277 NNNCTATAACAAAAATACCA NGG 2 0.362 Tier III
21 NC_000001.11 1 248535312 + LOC105373277 NNNCTATAACAAAAATACCA NGG 2 0.362 Tier III
22 NC_000004.12 4 177513133 + AGA-DT NNNCTATAACAAAAATACCA NGG 2 0.362 Tier III
23 NC_000004.12 4 88358549 - LOC124900731 NNNCTATCACAACAGCACCA NGG 2 0.2857 Tier III
24 NC_000003.12 3 65780450 + LOC107986018 NNNCTATAACCACAGTACTA NGG 2 0.1846 Tier III
25 NC_000012.12 12 82232361 - LOC105369873 NNNCTATAATAACAGTCCCA NGG 2 0.1661 Tier III
26 NC_000002.12 2 78423831 - LOC124906027 NNNCTATAACAAAAGTACCA NAG 2 0.0997 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 3756319 + Lyn NNNCTATAACAACAGCACAA NGG 2 0.2857 Tier I
2 NC_000067.6 1 191392921 - Ppp2r5a NNNCTGTAACAACAGTACCA NGG 1 0.7143 Tier II
3 NC_000070.6 4 120279667 + Foxo6 NNNCCACAACAACAGTACCA NGG 2 0.6875 Tier II
4 NC_000072.6 6 85291332 - Sfxn5 NNNTTATAACAGCAGTACCA NGG 2 0.5778 Tier II
5 NC_000071.6 5 98527833 + Cfap299 NNNTTATAACAACAGTACCT NGG 2 0.48 Tier II
6 NC_000084.6 18 19977883 - Dsc3 NNNCTATAACAGCAGTACCT NGG 2 0.4333 Tier II
7 NC_000084.6 18 19977974 - Dsc3 NNNCTATAACAGCAGTACCT NGG 2 0.4333 Tier II
8 NC_000083.6 17 5203194 - Arid1b NNNCAATGACAACAGTACCA NGG 2 0.3667 Tier II
9 NC_000068.7 2 121107591 + Tgm7 NNNCTATAACACCAGTACCA NGG 1 0.2632 Tier II
10 NC_000085.6 19 38911436 - Tbc1d12 NNNCTATAACACCAGTACCA NGG 1 0.2632 Tier II
11 NC_000067.6 1 134432649 + Adipor1 NNNCTATAACACCAATACCA NGG 2 0.2477 Tier II
12 NC_000085.6 19 7502235 - Atl3 NNNCTATAACAACAATACCA NAG 2 0.244 Tier II
13 NC_000068.7 2 38952993 - Olfml2a NNNCTGTAACTACAGTACCA NGG 2 0.2198 Tier II
14 NC_000070.6 4 122841511 - Ppt1 NNNCTATAACTACAGCACCA NGG 2 0.2051 Tier II
15 NC_000068.7 2 166934019 - Cse1l NNNCTGTAACACCAGTACCA NGG 2 0.188 Tier II
16 NC_000075.6 9 74113053 + Wdr72 NNNCTATAACACCAGCACCA NGG 2 0.1754 Tier II
17 NC_000087.7 Y 2168573 + Zfy2 NNNCTCTAACTACAGTACCA NGG 2 0.1399 Tier II
18 NC_000083.6 17 74665485 - Birc6 NNNCCATAACAACAGTTCCA NGG 2 0.1333 Tier II
19 NC_000086.7 X 105908935 - Atrx NNNCCATAACAACAGTTCCA NGG 2 0.1333 Tier II
20 NC_000085.6 19 37111383 - Cpeb3 NNNCTATAACAACAGTTCCA NGG 1 0.1333 Tier II
21 NC_000076.6 10 59003868 + Sh3rf3 NNNCTATAACAACAATTCCA NGG 2 0.1255 Tier II
22 NC_000077.6 11 6152103 + Nudcd3 NNNTTATAACAACAGTTCCA NGG 2 0.1067 Tier II
23 NC_000069.6 3 51870175 - Maml3 NNNCTATAACAACAGTGCCT NGG 2 0.1059 Tier II
24 NC_000076.6 10 105546540 + Tmtc2 NNNCTATAACCCCAGTACCA NGG 2 0.1053 Tier II
25 NC_000081.6 15 81321283 - Slc25a17 NNNCTATAACCCCAGTACCA NGG 2 0.1053 Tier II
26 NC_000083.6 17 80554523 - Cdkl4 NNNCTATGACAACAGTTCCA NGG 2 0.0978 Tier II
27 NC_000070.6 4 40974379 + Nfx1 NNNCTGTAACAACAGTTCCA NGG 2 0.0952 Tier II
28 NC_000076.6 10 26857055 + Arhgap18 NNNCTGTAACAACAGTTCCA NGG 2 0.0952 Tier II
29 NC_000077.6 11 77768905 + Myo18a NNNCTGTAACAACAGTTCCA NGG 2 0.0952 Tier II
30 NC_000081.6 15 25879239 + Retreg1 NNNCTATAACCACAGTACCC NGG 2 0.0909 Tier II
31 NC_000081.6 15 80150811 - Tab1 NNNCTATAACTCCAGTACCA NGG 2 0.081 Tier II
32 NC_000077.6 11 35599523 - Slit3 NNNCTATAAGAACAGTCCCA NGG 2 0.0686 Tier II
33 NC_000073.6 7 101950863 - Numa1 NNNCTCTAACAACAGTTCCA NGG 2 0.0606 Tier II
34 NC_000084.6 18 7453835 + Mpp7 NNNCTATAACACCAGTACCC NGG 2 0.0598 Tier II
35 NC_000075.6 9 18607134 - Muc16 NNNCTATAACTACAGTCCCA NGG 2 0.0543 Tier II
36 NC_000086.7 X 168369032 + Frmpd4 NNNCTATAAGAACAGTTCCA NGG 2 0.0519 Tier II
37 NC_000077.6 11 32664970 - Fbxw11 NNNCTATAACACCAGTGCCA NGG 2 0.0464 Tier II
38 NC_000068.7 2 12265928 - Itga8 NNNCTATAACATCAGTTCCA NGG 2 0.0444 Tier II
39 NC_000068.7 2 131336329 - Rnf24 NNNCTATAACATCAGTTCCA NGG 2 0.0444 Tier II
40 NC_000081.6 15 43066517 + Rspo2 NNNCTATAACATCAGTTCCA NGG 2 0.0444 Tier II
41 NC_000071.6 5 142087730 - Sdk1 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
42 NC_000071.6 5 143029269 + Rnf216 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
43 NC_000072.6 6 38600272 - Luc7l2 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
44 NC_000073.6 7 90541530 + Dlg2 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
45 NC_000075.6 9 31329454 + Prdm10 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
46 NC_000075.6 9 65439081 - Mtfmt NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
47 NC_000076.6 10 74516393 + Pcdh15 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
48 NC_000077.6 11 3439572 + Rnf185 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
49 NC_000079.6 13 4446849 - Akr1c6 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
50 NC_000079.6 13 97958703 + Arhgef28 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
51 NC_000080.6 14 56796481 + Zmym5 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
52 NC_000080.6 14 118998621 - Uggt2 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier II
53 NC_000071.6 5 115041776 + Sppl3 NNNCTATAACACCAGTTCCA NGG 2 0.0351 Tier II
54 NC_000070.6 4 32447868 + Bach2 NNNCTATAACACCAGTACCA NGA 2 0.0183 Tier II
55 NC_000075.6 9 24445740 + Dpy19l1 NNNCTATGACAACAGTACCA NGC 2 0.0163 Tier II
56 NC_000071.6 5 124617818 - Tctn2 NNNCCATAACAACAGTACCA NGT 2 0.0161 Tier II
57 NC_000083.6 17 5203194 - Gm34854 NNNCAATGACAACAGTACCA NGG 2 0.3667 Tier III
58 NC_000070.6 4 3498982 - Gm11787 NNNCTATAACAACAGCACAA NGG 2 0.2857 Tier III
59 NC_000083.6 17 39892378 - AY036118 NNNCTGTAACACCAGTACCA NGG 2 0.188 Tier III
60 NC_000074.6 8 121910821 + BC048644 NNNCTATCACATCAGTACCA NGG 2 0.1429 Tier III
61 NC_000070.6 4 99321207 + Gm35379 NNNCTATAACAGCAGTTCCA NGG 2 0.0963 Tier III
62 NC_000067.6 1 42738056 + Gm46091 NNNCTGTAACAACAGTTCCA NGG 2 0.0952 Tier III
63 NC_000072.6 6 30306956 - 1700095J07Rik NNNCTGTAACAACAGTTCCA NGG 2 0.0952 Tier III
64 NC_000068.7 2 137315655 - Gm14055 NNNCTATACCAACAGTAGCA NGG 2 0.0762 Tier III
65 NC_000077.6 11 102738950 - Gm39501 NNNCTATAACAACAGTTCTA NGG 2 0.0615 Tier III
66 NC_000075.6 9 112273662 + 2900079G21Rik NNNCTATAACATCAGTGCCA NGG 2 0.0588 Tier III
67 NC_000075.6 9 52742842 - Gm1715 NNNCTATAACTACAGTCCCA NGG 2 0.0543 Tier III
68 NC_000080.6 14 111642736 - Gm30439 NNNCTATAACTACAGTTCCA NGG 2 0.041 Tier III
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Other clones with same target sequence:

(none)