Construct: sgRNA BRDN0001145320
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGAGCTGCTACGTTTCGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GUCY2F (2986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77256
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
109453593 |
- |
GUCY2F |
NNNAGCTGCTACGTTTCGGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
130884135 |
- |
ABL1 |
NNNAGCTGCTGCGTTTGGGA |
NGG |
2 |
0.0382 |
Tier I |
| 3 |
NC_000020.11 |
20 |
20687700 |
+ |
RALGAPA2 |
NNNAGCTGCTAGGTTTGGGA |
NGG |
2 |
0.0261 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
112450106 |
+ |
Gm6588 |
NNNAGCTGCTAAGTTTCGGA |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000071.6 |
5 |
112450106 |
+ |
Sez6l |
NNNAGCTGCTAAGTTTCGGA |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000081.6 |
15 |
80631505 |
+ |
Grap2 |
NNNAGCTGCTACATTTCAGA |
NGG |
2 |
0.6391 |
Tier II |
| 4 |
NC_000081.6 |
15 |
94675014 |
+ |
Tmem117 |
NNNAGCTGCAACTTTTCGGA |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000073.6 |
7 |
79425690 |
+ |
Fanci |
NNNAGCTGCTACCTTTTGGA |
NGG |
2 |
0.1965 |
Tier II |
| 6 |
NC_000070.6 |
4 |
136651099 |
+ |
Ephb2 |
NNNAGCTGCTACGTTTTGGA |
NTG |
2 |
0.0182 |
Tier II |
Other clones with same target sequence:
(none)
