Construct: sgRNA BRDN0001145322
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGAGATCACACCTCGGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHB3 (2049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75946
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
184576859 |
- |
EPHB3 |
NNNGAGATCACACCTCGGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
27432851 |
+ |
IL21R |
NNNGAGGTCACACCTAGGGG |
NGG |
2 |
0.7059 |
Tier II |
| 3 |
NC_000019.10 |
19 |
13875952 |
+ |
NANOS3 |
NNNGAGATCTCATCTCGGGG |
NGG |
2 |
0.6176 |
Tier II |
| 4 |
NC_000004.12 |
4 |
8030556 |
- |
ABLIM2 |
NNNGACAGCACACCTCGGGG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000005.10 |
5 |
73808294 |
+ |
ARHGEF28 |
NNNGAATTCACACCTCGGGG |
NGG |
2 |
0.4375 |
Tier II |
| 6 |
NC_000015.10 |
15 |
25843246 |
- |
ATP10A |
NNNGAGATCACACCCCGGGA |
NGG |
2 |
0.2557 |
Tier II |
| 7 |
NC_000015.10 |
15 |
45690121 |
- |
SQOR |
NNNGAGATCACACCACGGGC |
NGG |
2 |
0.2481 |
Tier II |
| 8 |
NC_000002.12 |
2 |
218294137 |
- |
PNKD |
NNNGAGATCACACCTTGGGT |
NGG |
2 |
0.2154 |
Tier II |
| 9 |
NC_000023.11 |
X |
72114926 |
- |
NHSL2 |
NNNGAGATCACAGCTCGGAG |
NGG |
2 |
0.0974 |
Tier II |
| 10 |
NC_000022.11 |
22 |
39091937 |
+ |
LOC101927202 |
NNNGAGATCACACCTAGGAG |
NGG |
2 |
0.7143 |
Tier III |
| 11 |
NC_000007.14 |
7 |
92425469 |
+ |
TMBIM7P |
NNNGAGACCACACCTCCGGG |
NGG |
2 |
0.2353 |
Tier III |
| 12 |
NC_000002.12 |
2 |
62443217 |
- |
LOC105374764 |
NNNGAGATCACACCACGGGG |
NAG |
2 |
0.1501 |
Tier III |
| 13 |
NC_000002.12 |
2 |
46821668 |
+ |
LINC01118 |
NNNGAGATCACACCTGGGGA |
NGG |
2 |
0.1442 |
Tier III |
| 14 |
NC_000023.11 |
X |
115957456 |
+ |
DANT2 |
NNNGAGATCACACCTCTCGG |
NGG |
2 |
0.119 |
Tier III |
| 15 |
NC_000011.10 |
11 |
50073043 |
+ |
LOC124902810 |
NNNGAGCTCACACCTCTGGG |
NGG |
2 |
0.1094 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
21218032 |
- |
Ephb3 |
NNNGAGATCACACCCCGGGG |
NGG |
1 |
0.2727 |
Tier I |
| 2 |
NC_000070.6 |
4 |
102097640 |
- |
Pde4b |
NNNGAGATCACACCTCAAGG |
NGG |
2 |
0.6462 |
Tier II |
| 3 |
NC_000072.6 |
6 |
134076340 |
- |
Etv6 |
NNNGAGAACACACCTCGTGG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000070.6 |
4 |
154429480 |
+ |
Prdm16 |
NNNGAGATCACATCTCGGTG |
NGG |
2 |
0.4667 |
Tier II |
| 5 |
NC_000080.6 |
14 |
121265046 |
+ |
Farp1 |
NNNGAGATCACACCTCAGGG |
NAG |
2 |
0.242 |
Tier II |
| 6 |
NC_000071.6 |
5 |
113945951 |
+ |
Ssh1 |
NNNGAGATGACACCTCTGGG |
NGG |
2 |
0.1548 |
Tier II |
| 7 |
NC_000074.6 |
8 |
89042044 |
+ |
Sall1 |
NNNGAGATCGCCCCTCGGGG |
NGG |
2 |
0.1462 |
Tier II |
| 8 |
NC_000071.6 |
5 |
149579859 |
- |
Wdr95 |
NNNGAGATCACACTTCGGGG |
NTG |
2 |
0.0286 |
Tier II |
| 9 |
NC_000074.6 |
8 |
89042044 |
+ |
Gm3134 |
NNNGAGATCGCCCCTCGGGG |
NGG |
2 |
0.1462 |
Tier III |
| 10 |
NC_000086.7 |
X |
11519787 |
- |
Gm39496 |
NNNGAGATTACACCTGGGGG |
NGG |
2 |
0.1346 |
Tier III |
| 11 |
NC_000071.6 |
5 |
149579859 |
- |
Gm35763 |
NNNGAGATCACACTTCGGGG |
NTG |
2 |
0.0286 |
Tier III |
Other clones with same target sequence:
(none)
