Construct: sgRNA BRDN0001145324
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGCTTTGCGATTCACAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FLT3 (2322)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77348
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28052555 |
- |
FLT3 |
NNNGCTTTGCGATTCACAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
24214173 |
- |
SPATA13 |
NNNGCTTTGTGATTCACAGA |
NGG |
2 |
0.8824 |
Tier II |
| 3 |
NC_000006.12 |
6 |
70179606 |
- |
COL19A1 |
NNNGCTTTGAGATTCACAAG |
NGG |
2 |
0.619 |
Tier II |
| 4 |
NC_000009.12 |
9 |
70641582 |
+ |
TRPM3 |
NNNGCTGTGCGGTTCACAGG |
NGG |
2 |
0.4248 |
Tier II |
| 5 |
NC_000023.11 |
X |
31399057 |
+ |
DMD |
NNNGCTTTGCCATTCACAGA |
NGG |
2 |
0.4018 |
Tier II |
| 6 |
NC_000005.10 |
5 |
113055498 |
+ |
MCC |
NNNGCTTTGCTGTTCACAGG |
NGG |
2 |
0.2778 |
Tier II |
| 7 |
NC_000003.12 |
3 |
40005928 |
+ |
MYRIP |
NNNGCTTTGAGAGTCACAGG |
NGG |
2 |
0.2261 |
Tier II |
| 8 |
NC_000022.11 |
22 |
32598257 |
- |
SYN3 |
NNNGCTTTGTGATGCACAGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000018.10 |
18 |
42564175 |
+ |
LINC00907 |
NNNGCTTGGTGATTCACAGG |
NGG |
2 |
0.6902 |
Tier III |
| 10 |
NC_000012.12 |
12 |
74213435 |
+ |
LINC02882 |
NNNGCTTTGCTATTCACAGA |
NGG |
2 |
0.3606 |
Tier III |
| 11 |
NC_000008.11 |
8 |
29759893 |
+ |
LINC02099 |
NNNGCTTTGGGATTCACAAG |
NGG |
2 |
0.2778 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
168525718 |
- |
Nfatc2 |
NNNGCTTTGTGATTCACAGC |
NGG |
2 |
0.4034 |
Tier II |
| 2 |
NC_000077.6 |
11 |
113909855 |
+ |
Sdk2 |
NNNGCTCTGCGATTCACAGG |
NAG |
2 |
0.1782 |
Tier II |
| 3 |
NC_000074.6 |
8 |
91849513 |
+ |
Irx3os |
NNNGCTATGCCATTCACAGG |
NGG |
2 |
0.375 |
Tier III |
| 4 |
NC_000077.6 |
11 |
79184236 |
+ |
Gm30413 |
NNNGCTTTGCTCTTCACAGG |
NGG |
2 |
0.1012 |
Tier III |
| 5 |
NC_000071.6 |
5 |
25617536 |
+ |
Gm42235 |
NNNGCTTTGCCATGCACAGG |
NGG |
2 |
0.0 |
Tier III |
| 6 |
NC_000071.6 |
5 |
25617536 |
+ |
Gm46903 |
NNNGCTTTGCCATGCACAGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
