Construct: sgRNA BRDN0001145330
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGTTCCACCACTAGCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ULK4 (54986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76010
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
41938121 |
+ |
ULK4 |
NNNGTTCCACCACTAGCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
86716691 |
+ |
LDB3 |
NNNGTGCCACCACTTGCCAG |
NGG |
2 |
0.1143 |
Tier I |
| 3 |
NC_000019.10 |
19 |
9664472 |
+ |
ZNF562 |
NNNGTTCCACCACTGGCCAG |
NGG |
1 |
0.65 |
Tier II |
| 4 |
NC_000016.10 |
16 |
10060452 |
+ |
GRIN2A |
NNNGTTCCTCCGCTAGCCAG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000016.10 |
16 |
19709231 |
+ |
KNOP1 |
NNNGTCCCACCACTAGACAG |
NGG |
2 |
0.4242 |
Tier II |
| 6 |
NC_000021.9 |
21 |
44420950 |
+ |
TRPM2 |
NNNCTTCCACCACTGGCCAG |
NGG |
2 |
0.3441 |
Tier II |
| 7 |
NC_000020.11 |
20 |
21515226 |
- |
NKX2-2 |
NNNGCTCCACCTCTAGCCAG |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000012.12 |
12 |
41515097 |
+ |
PDZRN4 |
NNNCTTCCCCCACTAGCCAG |
NGG |
2 |
0.3025 |
Tier II |
| 9 |
NC_000002.12 |
2 |
227110364 |
+ |
COL4A4 |
NNNGTTCCATCACTAGCCAG |
NAG |
2 |
0.244 |
Tier II |
| 10 |
NC_000011.10 |
11 |
120820621 |
- |
GRIK4 |
NNNGTTCCAGCAGTAGCCAG |
NGG |
2 |
0.053 |
Tier II |
| 11 |
NC_000005.10 |
5 |
78145436 |
- |
AP3B1 |
NNNGTTCCACCACTAACCAG |
NTG |
2 |
0.039 |
Tier II |
| 12 |
NC_000005.10 |
5 |
176627138 |
+ |
SNCB |
NNNGTTCCACCACTAGGAAG |
NGG |
2 |
0.0317 |
Tier II |
| 13 |
NC_000023.11 |
X |
70703144 |
- |
TEX11 |
NNNGTTCCACCATTAGCCAG |
NTG |
2 |
0.0273 |
Tier II |
| 14 |
NC_000010.11 |
10 |
119384619 |
+ |
GRK5 |
NNNGTTCCTCCACTAGCCAG |
NGC |
2 |
0.0133 |
Tier II |
| 15 |
NC_000010.11 |
10 |
73838365 |
+ |
CAMK2G |
NNNGTTCCACCACTTGCCAG |
NTG |
2 |
0.0078 |
Tier II |
| 16 |
NC_000007.14 |
7 |
135670928 |
- |
STMP1 |
NNNGTTCCACCACTATCAAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000007.14 |
7 |
34502221 |
+ |
NPSR1-AS1 |
NNNGTCCCACCACTAGCAAG |
NGG |
2 |
0.4895 |
Tier III |
| 18 |
NC_000021.9 |
21 |
18583056 |
+ |
MIR548XHG |
NNNGTTCCACCACTGGCAAG |
NGG |
2 |
0.35 |
Tier III |
| 19 |
NC_000021.9 |
21 |
44420950 |
+ |
TRPM2-AS |
NNNCTTCCACCACTGGCCAG |
NGG |
2 |
0.3441 |
Tier III |
| 20 |
NC_000008.11 |
8 |
123761094 |
- |
LOC105375739 |
NNNCTGCCACCACTAGCCAG |
NGG |
2 |
0.3025 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
92820459 |
+ |
Rab30 |
NNNGTGCCACCACTAACCAG |
NGG |
2 |
0.5714 |
Tier II |
| 2 |
NC_000072.6 |
6 |
119085586 |
+ |
Cacna1c |
NNNGTGCCACCACTAGCCAA |
NGG |
2 |
0.5357 |
Tier II |
| 3 |
NC_000070.6 |
4 |
148836109 |
- |
Casz1 |
NNNGTTCCACCACATGCCAG |
NGG |
2 |
0.1238 |
Tier II |
| 4 |
NC_000068.7 |
2 |
166589175 |
- |
Prex1 |
NNNGTTCCACCCCCAGCCAG |
NGG |
2 |
0.0752 |
Tier II |
| 5 |
NC_000078.6 |
12 |
55837821 |
+ |
Brms1l |
NNNGTTCCACCACTAAGCAG |
NGG |
2 |
0.0588 |
Tier II |
| 6 |
NC_000080.6 |
14 |
66251702 |
+ |
Ptk2b |
NNNGTGCCACCACTACCCAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000081.6 |
15 |
94871838 |
+ |
Tmem117 |
NNNGTTCCACCACTGCCCAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000086.7 |
X |
150622799 |
+ |
Pfkfb1 |
NNNGTTCCACCATGAGCCAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000084.6 |
18 |
41794334 |
+ |
Gm50410 |
NNNGTTCGGCCACTAGCCAG |
NGG |
2 |
0.4286 |
Tier III |
| 10 |
NC_000078.6 |
12 |
79945747 |
+ |
4933435C09Rik |
NNNTTTCCACCCCTAGCCAG |
NGG |
2 |
0.0957 |
Tier III |
Other clones with same target sequence:
(none)
