Construct: sgRNA BRDN0001145332
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCAGAGGAGAATCGGCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CMPK1 (51727)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77302
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
47334014 |
- |
CMPK1 |
NNNAGAGGAGAATCGGCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
30721892 |
+ |
PCDH7 |
NNNGGAGGAGAATCGGCCGG |
NGG |
1 |
0.625 |
Tier I |
| 3 |
NC_000020.11 |
20 |
60033643 |
+ |
CDH26 |
NNNAGAGGAGAATCGGCAGG |
NGG |
1 |
0.5385 |
Tier I |
| 4 |
NC_000019.10 |
19 |
608521 |
+ |
HCN2 |
NNNGGAGGAGAAACGGCCGG |
NGG |
2 |
0.4327 |
Tier II |
| 5 |
NC_000012.12 |
12 |
52449925 |
- |
KRT6B |
NNNAGAGGAGAATTGGCAGG |
NGG |
2 |
0.3949 |
Tier II |
| 6 |
NC_000006.12 |
6 |
166774978 |
+ |
RPS6KA2 |
NNNAGAGGAGAAACGGCAGG |
NGG |
2 |
0.3728 |
Tier II |
| 7 |
NC_000002.12 |
2 |
30232557 |
+ |
LBH |
NNNAGAGGAGAAGCGGCCGG |
NGG |
1 |
0.2609 |
Tier II |
| 8 |
NC_000001.11 |
1 |
245241310 |
+ |
KIF26B |
NNNAGAGGAAAAGCGGCCGG |
NGG |
2 |
0.2435 |
Tier II |
| 9 |
NC_000002.12 |
2 |
26271192 |
- |
HADHB |
NNNAGAGAAGAATCGGCCGG |
NCG |
2 |
0.1071 |
Tier II |
| 10 |
NC_000004.12 |
4 |
41214291 |
+ |
APBB2 |
NNNAGAGGAGAAGCCGCCGG |
NGG |
2 |
0.0711 |
Tier II |
| 11 |
NC_000007.14 |
7 |
18360045 |
- |
HDAC9 |
NNNAGAGGAGAATCGGAGGG |
NGG |
2 |
0.0622 |
Tier II |
| 12 |
NC_000007.14 |
7 |
98401076 |
+ |
BAIAP2L1 |
NNNAGAGGAGAAGCGGCCGG |
NCG |
2 |
0.028 |
Tier II |
| 13 |
NC_000008.11 |
8 |
66956925 |
+ |
TCF24 |
NNNAGAGGAGAATCGGGCCG |
NGG |
2 |
0.0264 |
Tier II |
| 14 |
NC_000011.10 |
11 |
70143738 |
- |
ANO1 |
NNNACAGGAGAATGGGCCGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000022.11 |
22 |
49838761 |
+ |
LOC105377205 |
NNNAGAGGGGAACCGGCCGG |
NGG |
2 |
0.5263 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
167167132 |
+ |
Kcnb1 |
NNNAGAGGAGAACCGGCCTG |
NGG |
2 |
0.5263 |
Tier II |
| 2 |
NC_000068.7 |
2 |
179680693 |
- |
Cdh4 |
NNNAGAGGAGAATAGGCTGG |
NGG |
2 |
0.225 |
Tier II |
| 3 |
NC_000068.7 |
2 |
91237121 |
- |
Ddb2 |
NNNAGAGGAGGAGCGGCCGG |
NGG |
2 |
0.1696 |
Tier II |
| 4 |
NC_000073.6 |
7 |
81859343 |
+ |
Tm6sf1 |
NNNAGAGGAGACGCGGCCGG |
NGG |
2 |
0.0686 |
Tier II |
| 5 |
NC_000077.6 |
11 |
51539366 |
+ |
Col23a1 |
NNNAGAGGAGAATGGGCCGG |
NTG |
2 |
0.0 |
Tier II |
| 6 |
NC_000071.6 |
5 |
92571124 |
- |
Fam47e |
NNNAGAGGAGAATGGGCTGG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
