Construct: sgRNA BRDN0001145335
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGCCACAGTCCGGCACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FLT1 (2321)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75556
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28334056 |
+ |
FLT1 |
NNNGCCACAGTCCGGCACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
158526484 |
- |
PTPRN2 |
NNNGCCACAGTCCAGCATGT |
NGG |
2 |
0.4821 |
Tier II |
| 3 |
NC_000002.12 |
2 |
120253073 |
- |
RALB |
NNNGCCACAGCCCGGCACGG |
NGG |
2 |
0.1176 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
147594587 |
+ |
Flt1 |
NNNGCCACAGTCCGGCAGGT |
NGG |
1 |
0.1333 |
Tier I |
| 2 |
NC_000072.6 |
6 |
145404369 |
+ |
Lmntd1 |
NNNGACACAGTCTGGCACGT |
NGG |
2 |
0.4 |
Tier II |
| 3 |
NC_000082.6 |
16 |
43223703 |
- |
Zbtb20 |
NNNGCCACAGTCTGGCACGT |
NAG |
2 |
0.1815 |
Tier II |
| 4 |
NC_000070.6 |
4 |
135764047 |
- |
Myom3 |
NNNGCCACACTCAGGCACGT |
NGG |
2 |
0.1538 |
Tier II |
| 5 |
NC_000078.6 |
12 |
54026191 |
- |
Npas3 |
NNNGCCACAGTCAGGCACGT |
NAG |
2 |
0.0997 |
Tier II |
| 6 |
NC_000082.6 |
16 |
28888776 |
- |
Mb21d2 |
NNNGCCAGAGTCCGGCACGC |
NGG |
2 |
0.0584 |
Tier II |
| 7 |
NC_000081.6 |
15 |
36944958 |
- |
Gm34590 |
NNNTCCACAGTCCGGCACGC |
NGG |
2 |
0.0331 |
Tier III |
Other clones with same target sequence:
(none)
