Construct: sgRNA BRDN0001145338

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGACTGAAAAAACTCCCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53RK (112858)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77699

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 46687108 - TP53RK NNNACTGAAAAAACTCCCCA NGG 0 1.0 Tier I
2 NC_000004.12 4 5990425 - C4orf50 NNNTCTGAAAAAACACCCCA NGG 2 0.3684 Tier I
3 NC_000023.11 X 20176255 - RPS6KA3 NNNACTGCAAAAACTCCCAA NGG 2 0.1837 Tier I
4 NC_000002.12 2 216478574 + SMARCAL1 NNNACTGACAAAACTCCCCA NGG 1 0.5714 Tier II
5 NC_000015.10 15 80569477 + ARNT2 NNNTCTGAGAAAACTCCCCA NGG 2 0.4242 Tier II
6 NC_000019.10 19 40314491 + C19orf47 NNNACTGAAAGAGCTCCCCA NGG 2 0.4239 Tier II
7 NC_000006.12 6 165429418 - PDE10A NNNACTGAAAAAGCTCCCCT NGG 2 0.3913 Tier II
8 NC_000002.12 2 3422536 - TRAPPC12 NNNAGTTAAAAAACTCCCCA NGG 2 0.3429 Tier II
9 NC_000013.11 13 41696925 - VWA8 NNNACTGAAAGAACTCACCA NGG 2 0.3033 Tier II
10 NC_000004.12 4 140699109 + TBC1D9 NNNACTGAAAAAGCTCCCAA NGG 2 0.2795 Tier II
11 NC_000002.12 2 229400625 + DNER NNNACTGAAAGAACTCCCAA NGG 2 0.2786 Tier II
12 NC_000008.11 8 135576516 - KHDRBS3 NNNACTGAAAGAACTCCCAA NGG 2 0.2786 Tier II
13 NC_000011.10 11 107616729 - ELMOD1 NNNACTGAAAGAACTCCCAA NGG 2 0.2786 Tier II
14 NC_000011.10 11 57719392 - TMX2 NNNACTGAAAAAATTTCCCA NGG 2 0.2256 Tier II
15 NC_000007.14 7 31710743 + PDE1C NNNACTCAAAAATCTCCCCA NGG 2 0.2063 Tier II
16 NC_000010.11 10 114777786 + ABLIM1 NNNACTGACAAAAATCCCCA NGG 2 0.2 Tier II
17 NC_000015.10 15 86792244 - AGBL1 NNNACTGAACAAACACCCCA NGG 2 0.193 Tier II
18 NC_000005.10 5 129018333 - SLC27A6 NNNACTGAAAAAAATCACCA NGG 2 0.1633 Tier II
19 NC_000005.10 5 168328840 - WWC1 NNNAGTGAAAAAACTCCCCA NAG 2 0.1556 Tier II
20 NC_000001.11 1 20659479 + DDOST NNNACTGAAAAAACTCCTCC NGG 2 0.1461 Tier II
21 NC_000023.11 X 105602167 + IL1RAPL2 NNNACTGCACAAACTCCCCA NGG 2 0.1429 Tier II
22 NC_000007.14 7 111407683 - IMMP2L NNNCCTGAAAATACTCCCCA NGG 2 0.1176 Tier II
23 NC_000006.12 6 144850232 - UTRN NNNACTGGAAAAACTGCCCA NGG 2 0.1128 Tier II
24 NC_000009.12 9 116905125 + ASTN2 NNNACTGAAAAGACTGCCCA NGG 2 0.1111 Tier II
25 NC_000023.11 X 84440315 + HDX NNNACTGAGAAAACTGCCCA NGG 2 0.1026 Tier II
26 NC_000020.11 20 44954949 - TOMM34 NNNACTTAAAAAACTCCCGA NGG 2 0.0714 Tier II
27 NC_000004.12 4 80940705 - CFAP299 NNNACTGAAAAAACTCACGA NGG 2 0.0583 Tier II
28 NC_000007.14 7 43390998 + HECW1 NNNTCTGAAAAAACTCCCCA NGC 2 0.0141 Tier II
29 NC_000019.10 19 23803148 + RPSA2 NNNACTGAAAATACTCCCCA NTG 2 0.013 Tier II
30 NC_000015.10 15 27765382 + OCA2 NNNACTGAAGAAACTCCCCA NGC 2 0.0123 Tier II
31 NC_000001.11 1 86393279 + ODF2L NNNACTGAAAAAAGTCACCA NGG 2 0.0 Tier II
32 NC_000003.12 3 178992545 - LOC124906307 NNNTCTGAAAAAACTCCCCG NGG 2 0.4866 Tier III
33 NC_000002.12 2 13090825 - LOC105373436 NNNACTGAAAAAGCTCCCAA NGG 2 0.2795 Tier III
34 NC_000011.10 11 57719392 - TMX2-CTNND1 NNNACTGAAAAAATTTCCCA NGG 2 0.2256 Tier III
35 NC_000010.11 10 114777786 + LOC101927692 NNNACTGACAAAAATCCCCA NGG 2 0.2 Tier III
36 NC_000005.10 5 62086132 - LOC124900610 NNNACTGAAAAAACACCCCA NAG 2 0.1501 Tier III
37 NC_000001.11 1 29495379 - LOC107984933 NNNACTGAAAAATCTCCCAA NGG 2 0.1286 Tier III
38 NC_000008.11 8 47991920 - UBE2V2-AS1 NNNACTGAATAAACTCCCCA NTG 2 0.0344 Tier III
39 NC_000002.12 2 241808639 - LOC124905349 NNNTCTGAAAAAACTCCCCA NGC 2 0.0141 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 156067526 + Spag4 NNNACTGAATAAACTCCACA NGG 2 0.4751 Tier I
2 NC_000086.7 X 159337284 + Rps6ka3 NNNACTGCAAAAACTCCCAA NGG 2 0.1837 Tier I
3 NC_000086.7 X 123119283 + Cldn34c1 NNNAATGAAAAAACTCCCCA NGG 1 0.5714 Tier II
4 NC_000086.7 X 123348592 + Gm2411 NNNAATGAAAAAACTCCCCA NGG 1 0.5714 Tier II
5 NC_000086.7 X 123578086 + Cldn34c2 NNNAATGAAAAAACTCCCCA NGG 1 0.5714 Tier II
6 NC_000086.7 X 123790042 + Cldn34c3 NNNAATGAAAAAACTCCCCA NGG 1 0.5714 Tier II
7 NC_000086.7 X 124017816 + Gm5945 NNNAATGAAAAAACTCCCCA NGG 1 0.5714 Tier II
8 NC_000085.6 19 24471550 + Pip5k1b NNNACTGAAGAAACTACCCA NGG 2 0.5556 Tier II
9 NC_000069.6 3 122442437 - Bcar3 NNNACTATAAAAACTCCCCA NGG 2 0.4286 Tier II
10 NC_000074.6 8 14983390 - Arhgef10 NNNACTTAAAAAGCTCCCCA NGG 2 0.3727 Tier II
11 NC_000083.6 17 90251163 - Nrxn1 NNNACTGAAAAAAATACCCA NGG 2 0.35 Tier II
12 NC_000079.6 13 74648147 - Erap1 NNNATTGAAAAAACTCCACA NGG 2 0.3427 Tier II
13 NC_000082.6 16 38819171 - Tex55 NNNACTGAAAAAATTCTCCA NGG 2 0.3422 Tier II
14 NC_000080.6 14 11984234 + Ptprg NNNACTGAAACAATTCCCCA NGG 2 0.2933 Tier II
15 NC_000074.6 8 114616575 - Wwox NNNACTGAAGAAACTCACCA NGG 2 0.2593 Tier II
16 NC_000083.6 17 71776811 - Clip4 NNNACTGAAGAAACTCACCA NGG 2 0.2593 Tier II
17 NC_000079.6 13 34236156 + Slc22a23 NNNAATGAAAAAAATCCCCA NGG 2 0.2 Tier II
18 NC_000080.6 14 61200539 - Sacs NNNTCTGAAAAAACTTCCCA NGG 2 0.1958 Tier II
19 NC_000080.6 14 96353851 + Klhl1 NNNACTGAGAAAACTCCCCA NAG 2 0.1728 Tier II
20 NC_000073.6 7 133750433 + Dhx32 NNNACTTAAAAATCTCCCCA NGG 2 0.1714 Tier II
21 NC_000072.6 6 32890399 + Chchd3 NNNACAGAAAAAACTGCCCA NGG 2 0.1333 Tier II
22 NC_000068.7 2 109147658 - Mettl15 NNNACTGAAAAATCTTCCCA NGG 2 0.0923 Tier II
23 NC_000069.6 3 132928552 - Npnt NNNACTGAAAAAAATCCCCA NAG 2 0.0907 Tier II
24 NC_000079.6 13 33924076 + Serpinb6a NNNACTGAAAAAACTGCCAA NGG 2 0.0659 Tier II
25 NC_000083.6 17 81426480 + Slc8a1 NNNACTGAAAATACTGCCCA NGG 2 0.0513 Tier II
26 NC_000067.6 1 139637165 - Cfhr3 NNNACTGAAAAAAATCCCCA NGA 2 0.0243 Tier II
27 NC_000068.7 2 125325032 - Fbn1 NNNACTGAAACAACTCCCCA NTG 2 0.0156 Tier II
28 NC_000080.6 14 14082531 - Atxn7 NNNACTGAAAAAACTTCCCA NTG 2 0.012 Tier II
29 NC_000084.6 18 78375496 + Slc14a2 NNNACTGAAAAATCTCCCCA NTG 2 0.0117 Tier II
30 NC_000069.6 3 119166740 + Dpyd NNNACTGAACAAACTCCCCA NGC 2 0.0074 Tier II
31 NC_000068.7 2 26137621 + Tmem250-ps NNNACTGTAAAAACTCCCCA NGT 2 0.0069 Tier II
32 NC_000068.7 2 13414274 + Cubn NNNACTGAAACAACTCCCCA NGT 2 0.0065 Tier II
33 NC_000074.6 8 114616575 - Gm30554 NNNACTGAAGAAACTCACCA NGG 2 0.2593 Tier III
34 NC_000083.6 17 71776811 - Gm30140 NNNACTGAAGAAACTCACCA NGG 2 0.2593 Tier III
35 NC_000084.6 18 40786871 + Gm41710 NNNACTGAAGAAACTCCCTA NGG 2 0.2564 Tier III
36 NC_000069.6 3 34599189 - Sox2ot NNNAATGAAACAACTCCCCA NGG 2 0.2286 Tier III
37 NC_000069.6 3 34599189 - Gm42205 NNNAATGAAACAACTCCCCA NGG 2 0.2286 Tier III
38 NC_000079.6 13 15988981 + B230303A05Rik NNNAATGAAAACACTCCCCA NGG 2 0.1504 Tier III
39 NC_000083.6 17 87455629 - Gm30794 NNNACTGAAAGAACTCCGCA NGG 2 0.0867 Tier III
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Other clones with same target sequence:

(none)