Construct: sgRNA BRDN0001145342
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAGAGAGGAAAATATCCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAPSS2 (9060)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76316
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
87713230 |
+ |
PAPSS2 |
NNNGAGAGGAAAATATCCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
96551338 |
+ |
TM9SF3 |
NNNGAGAGGAAAATATCAGT |
NGG |
2 |
0.1615 |
Tier I |
| 3 |
NC_000002.12 |
2 |
128002564 |
+ |
SAP130 |
NNNAAGAGGAAAATATCAGC |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000008.11 |
8 |
26629447 |
- |
DPYSL2 |
NNNGAGAGGAAAATGTCCTC |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000002.12 |
2 |
28635735 |
- |
PLB1 |
NNNAAGAGGAAAATATCCCC |
NGG |
2 |
0.4034 |
Tier II |
| 6 |
NC_000011.10 |
11 |
117582681 |
+ |
DSCAML1 |
NNNGAGAGGGGAATATCCGC |
NGG |
2 |
0.3611 |
Tier II |
| 7 |
NC_000015.10 |
15 |
75390117 |
+ |
SIN3A |
NNNGAGAGGAAAATATCCTA |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000005.10 |
5 |
137186171 |
- |
SPOCK1 |
NNNGAGAGGAAAATATCTGA |
NGG |
2 |
0.3214 |
Tier II |
| 9 |
NC_000008.11 |
8 |
87346991 |
+ |
CNBD1 |
NNNGAGAGGAAAATATCTCC |
NGG |
2 |
0.2882 |
Tier II |
| 10 |
NC_000003.12 |
3 |
119945483 |
+ |
GSK3B |
NNNGAGAGGAAATTATCCTC |
NGG |
2 |
0.2 |
Tier II |
| 11 |
NC_000020.11 |
20 |
4222993 |
- |
ADRA1D |
NNNGATAGGAAAATATCCGT |
NGG |
2 |
0.2 |
Tier II |
| 12 |
NC_000021.9 |
21 |
40795226 |
- |
DSCAM |
NNNGAGAGGAAAATATCCAG |
NGG |
2 |
0.042 |
Tier II |
| 13 |
NC_000006.12 |
6 |
112297461 |
- |
LAMA4-AS1 |
NNNTAGAGGAAAATATCCGC |
NGG |
1 |
0.3636 |
Tier III |
| 14 |
NC_000006.12 |
6 |
112297461 |
- |
LOC107986632 |
NNNTAGAGGAAAATATCCGC |
NGG |
1 |
0.3636 |
Tier III |
| 15 |
NC_000023.11 |
X |
18029712 |
- |
LINC01456 |
NNNGAGAGGAAAATACCCCC |
NGG |
2 |
0.2989 |
Tier III |
| 16 |
NC_000012.12 |
12 |
23008805 |
+ |
LINC02955 |
NNNGAGTGGAAAATATTCGC |
NGG |
2 |
0.2042 |
Tier III |
| 17 |
NC_000021.9 |
21 |
26186920 |
- |
APP-DT |
NNNGAGAGGAAAACATCCTC |
NGG |
2 |
0.1905 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
32637189 |
+ |
Papss2 |
NNNGAGAAGAGAATATCCGC |
NGG |
2 |
0.65 |
Tier I |
| 2 |
NC_000078.6 |
12 |
78454225 |
- |
Gphn |
NNNGAGACGAAAATATCAGC |
NGG |
2 |
0.3314 |
Tier II |
| 3 |
NC_000079.6 |
13 |
105446298 |
- |
Htr1a |
NNNGACAGGAAAACATCCGC |
NGG |
2 |
0.1948 |
Tier II |
| 4 |
NC_000067.6 |
1 |
186623166 |
+ |
Tgfb2 |
NNNGAGAGGAAAATATCTGC |
NGA |
2 |
0.0446 |
Tier II |
| 5 |
NC_000068.7 |
2 |
66453671 |
- |
Gm13629 |
NNNGAGAGGGAAATATCCTC |
NGG |
2 |
0.3704 |
Tier III |
Other clones with same target sequence:
(none)
