Construct: sgRNA BRDN0001145349

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATTGATCATGAGCACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A1 (1457)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76674

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 495724 - CSNK2A1 NNNTGATCATGAGCACAGAA NGG 0 1.0 Tier I
2 NC_000015.10 15 75430378 - SIN3A NNNTGAGCATGAGCACAGAA NGA 2 0.0408 Tier I
3 NC_000011.10 11 11352615 - CSNK2A3 NNNTGATCATGAGCACAGAA NGC 1 0.0222 Tier I
4 NC_000003.12 3 186332235 + DGKG NNNTAAACATGAGCACAGAA NGG 2 0.7583 Tier II
5 NC_000015.10 15 65780207 - DENND4A NNNTGAACAAGAGCACAGAA NGG 2 0.75 Tier II
6 NC_000002.12 2 105401828 + FHL2 NNNTGTTCATGAGCAAAGAA NGG 2 0.7143 Tier II
7 NC_000022.11 22 20880146 - SNAP29 NNNTGACCATGAGCAAAGAA NGG 2 0.6875 Tier II
8 NC_000016.10 16 7661020 + RBFOX1 NNNGGATCATGAGCACAGAA NGG 1 0.625 Tier II
9 NC_000015.10 15 29059291 + APBA2 NNNTGATCCTAAGCACAGAA NGG 2 0.5714 Tier II
10 NC_000008.11 8 27287371 + TRIM35 NNNTGACCATGGGCACAGAA NGG 2 0.4965 Tier II
11 NC_000003.12 3 2509589 + CNTN4 NNNTGATAATGGGCACAGAA NGG 2 0.4694 Tier II
12 NC_000022.11 22 37376258 + ELFN2 NNNGGATCATGGGCACAGAA NGG 2 0.4514 Tier II
13 NC_000023.11 X 133411210 + GPC4 NNNTGCTCATGAACACAGAA NGG 2 0.4196 Tier II
14 NC_000001.11 1 197703607 + DENND1B NNNTGAACATGAGCACACAA NGG 2 0.4167 Tier II
15 NC_000006.12 6 33430473 - SYNGAP1 NNNTGATCCTGACCACAGAA NGG 2 0.2406 Tier II
16 NC_000020.11 20 61256528 - CDH4 NNNTGATCAAGAGCACAGCA NGG 2 0.1773 Tier II
17 NC_000016.10 16 70395896 + ST3GAL2 NNNGGATCATGAGCCCAGAA NGG 2 0.1705 Tier II
18 NC_000004.12 4 174664262 - GLRA3 NNNGGATCATGAGCACAGAA NAG 2 0.162 Tier II
19 NC_000002.12 2 140928182 + LRP1B NNNTGAACATGAGCAGAGAA NGG 2 0.1346 Tier II
20 NC_000010.11 10 49327386 + C10orf71 NNNTGATCATTTGCACAGAA NGG 2 0.1282 Tier II
21 NC_000005.10 5 111935178 - NREP NNNTGATCATGAGCACAGCT NGG 2 0.1241 Tier II
22 NC_000012.12 12 119019656 - SRRM4 NNNTGTTCATGAGCAGAGAA NGG 2 0.1099 Tier II
23 NC_000012.12 12 119637083 - TMEM233 NNNTGATCATGAGCACGGAT NGG 2 0.1059 Tier II
24 NC_000002.12 2 230436438 - SP100 NNNTTATCATGAGAACAGAA NGG 2 0.105 Tier II
25 NC_000002.12 2 214313800 + SPAG16 NNNTGACCATGAGCACTGAA NGG 2 0.0917 Tier II
26 NC_000008.11 8 4367698 + CSMD1 NNNTGACCATGAGCACTGAA NGG 2 0.0917 Tier II
27 NC_000007.14 7 72426372 - CALN1 NNNTGATCATGAGAACAGCA NGG 2 0.0724 Tier II
28 NC_000007.14 7 108559839 + THAP5 NNNTGATCATGAGAACAGCA NGG 2 0.0724 Tier II
29 NC_000019.10 19 57376218 + ZNF547 NNNTGATCATGAGAACAGCA NGG 2 0.0724 Tier II
30 NC_000023.11 X 84497005 - HDX NNNTGATCATGAGAACAGCA NGG 2 0.0724 Tier II
31 NC_000006.12 6 138226205 - ARFGEF3 NNNTGATGATGAGCACAGAA NCG 2 0.0689 Tier II
32 NC_000015.10 15 48126175 - SLC24A5 NNNTGATCATGAGCAGAGCA NGG 2 0.0318 Tier II
33 NC_000008.11 8 55538732 - XKR4 NNNTGATCATGAGTACAGAA NTG 2 0.0286 Tier II
34 NC_000011.10 11 11352615 - GALNT18 NNNTGATCATGAGCACAGAA NGC 1 0.0222 Tier II
35 NC_000007.14 7 33377657 + BBS9 NNNTGTTCATGAGCACAGAA NGT 2 0.0115 Tier II
36 NC_000016.10 16 68376923 + SMPD3 NNNTGATCATAAGGACAGAA NGG 2 0.0 Tier II
37 NC_000022.11 22 48561138 + TAFA5 NNNTGATCATAAGGACAGAA NGG 2 0.0 Tier II
38 NC_000020.11 20 59990367 + CDH26 NNNTGATCATGGGGACAGAA NGG 2 0.0 Tier II
39 NC_000003.12 3 59277833 + CFAP20DC-DT NNNTTATCAGGAGCACAGAA NGG 2 0.15 Tier III
40 NC_000012.12 12 58637081 + LOC100506869 NNNTGATCATTTGCACAGAA NGG 2 0.1282 Tier III
41 NC_000012.12 12 58637081 + LINC02388 NNNTGATCATTTGCACAGAA NGG 2 0.1282 Tier III
42 NC_000005.10 5 111935178 - NREP-AS1 NNNTGATCATGAGCACAGCT NGG 2 0.1241 Tier III
43 NC_000012.12 12 119637083 - PRKAB1-AS1 NNNTGATCATGAGCACGGAT NGG 2 0.1059 Tier III
44 NC_000002.12 2 13178588 + LOC105373436 NNNTTATCATGAGAACAGAA NGG 2 0.105 Tier III
45 NC_000002.12 2 13178588 + LOC105373484 NNNTTATCATGAGAACAGAA NGG 2 0.105 Tier III
46 NC_000001.11 1 116489115 - LOC112268235 NNNTGATAATGAGCAGAGAA NGG 2 0.1 Tier III
47 NC_000008.11 8 81845954 + LINC02235 NNNTGATCATGAGAACAGCA NGG 2 0.0724 Tier III
48 NC_000001.11 1 56245814 - LOC124904186 NNNTGATCATGTGCTCAGAA NGG 2 0.0667 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 152260838 + Csnk2a1 NNNTGATCATGAGCACAGAA NGG 0 1.0 Tier I
2 NC_000068.7 2 21576915 - Gpr158 NNNCGATCATCAGCACAGAA NGG 2 0.2773 Tier I
3 NC_000076.6 10 69712855 + Ank3 NNNTGATCATGAGCACAGAA NGG 0 1.0 Tier II
4 NC_000083.6 17 64708671 - Man2a1 NNNTGATCATAAACACAGAA NGG 2 0.9231 Tier II
5 NC_000068.7 2 41629405 - Lrp1b NNNTGATCAAGAGCAAAGAA NGG 2 0.8571 Tier II
6 NC_000070.6 4 65591720 + Astn2 NNNTGGACATGAGCACAGAA NGG 2 0.625 Tier II
7 NC_000082.6 16 40567713 - Lsamp NNNTGAAAATGAGCACAGAA NGG 2 0.5688 Tier II
8 NC_000080.6 14 63463891 + Fam167a NNNTGCTCATGAGCACAGAA NGG 1 0.4545 Tier II
9 NC_000068.7 2 59680776 - Tanc1 NNNGGATCATGAGCACAGAT NGG 2 0.375 Tier II
10 NC_000072.6 6 51929804 - Skap2 NNNTAATCATGACCACAGAA NGG 2 0.3649 Tier II
11 NC_000069.6 3 99312984 - Tbx15 NNNAGATCATGAGCACAGAG NGG 2 0.3641 Tier II
12 NC_000068.7 2 96717074 + Lrrc4c NNNTGATCTTGAGCACAGTA NGG 2 0.3231 Tier II
13 NC_000069.6 3 131241435 - Hadh NNNTGCTCGTGAGCACAGAA NGG 2 0.303 Tier II
14 NC_000084.6 18 9788206 - Colec12 NNNTGATCATCAGCACATAA NGG 2 0.2857 Tier II
15 NC_000070.6 4 129333804 - Rbbp4 NNNTGATCCTCAGCACAGAA NGG 2 0.2449 Tier II
16 NC_000075.6 9 122987516 - Kif15 NNNTGATCCTCAGCACAGAA NGG 2 0.2449 Tier II
17 NC_000067.6 1 152619608 + Rgl1 NNNTGCTCAGGAGCACAGAA NGG 2 0.2273 Tier II
18 NC_000068.7 2 164179678 + Wfdc5 NNNTCATCATGAGCACAGCA NGG 2 0.1626 Tier II
19 NC_000068.7 2 24006319 + Hnmt NNNTGATCATGTGCACACAA NGG 2 0.1587 Tier II
20 NC_000083.6 17 30194241 + Zfand3 NNNTGACCATGAGCTCAGAA NGG 2 0.1375 Tier II
21 NC_000067.6 1 38874804 - Chst10 NNNTGAGCATGAGCACAGCA NGG 2 0.1217 Tier II
22 NC_000079.6 13 9466605 + Dip2c NNNTGATCCTGAGCTCAGAA NGG 2 0.1143 Tier II
23 NC_000068.7 2 34492919 + Mapkap1 NNNTGATCATGAGATCAGAA NGG 2 0.07 Tier II
24 NC_000077.6 11 95362503 - Fam117a NNNTGACCATGAGCACAGAA NGA 2 0.0477 Tier II
25 NC_000069.6 3 13567268 + Ralyl NNNTGATCATGAGCTGAGAA NGG 2 0.0308 Tier II
26 NC_000079.6 13 96069686 + Sv2c NNNTGATCATCAGCACAGAA NGA 2 0.0298 Tier II
27 NC_000070.6 4 109357969 + Eps15 NNNTGGTCATGAGCACAGAA NTG 2 0.0278 Tier II
28 NC_000072.6 6 97555840 - Frmd4b NNNCGATCATGAGCACAGAA NTG 2 0.0252 Tier II
29 NC_000084.6 18 77424835 - Loxhd1 NNNTGATCATGAGCACAGAT NTG 2 0.0234 Tier II
30 NC_000078.6 12 114142335 - Igh NNNTCATCATGAGCACAGAT NGG 2 0.4714 Tier III
31 NC_000069.6 3 113189484 - Amy2-ps1 NNNTGATCATGGGCACAGAC NGG 2 0.1641 Tier III
32 NC_000068.7 2 164179678 + A730032A03Rik NNNTCATCATGAGCACAGCA NGG 2 0.1626 Tier III
33 NC_000080.6 14 52848895 - Tcra NNNTGATCATGGGCAGAGAA NGG 2 0.1111 Tier III
34 NC_000080.6 14 53159935 - Tcra NNNTGATCATGGGCAGAGAA NGG 2 0.1111 Tier III
35 NC_000067.6 1 41939347 + Gm29260 NNNTGCTCATGAGCACAGAA NGA 2 0.0316 Tier III
36 NC_000070.6 4 98192112 - Gm12691 NNNTGATCATGACCACAGAA NGA 2 0.0292 Tier III
37 NC_000070.6 4 109357969 + Gm52702 NNNTGGTCATGAGCACAGAA NTG 2 0.0278 Tier III
38 NC_000067.6 1 156524736 + Csnk2a3 NNNTGATCATGAGCACAGAA NGC 1 0.0222 Tier III
39 NC_000070.6 4 75325006 + Gm11255 NNNTGATCATGAGCACAGAA NGC 1 0.0222 Tier III
40 NC_000080.6 14 14197800 + Csnk2a1-ps1 NNNTGATCATGAGCACAGAA NGC 1 0.0222 Tier III
41 NC_000086.7 X 16610761 + Csnk2a1-ps NNNTGATCATGAGCACAGAA NGC 1 0.0222 Tier III
42 NC_000081.6 15 74460529 + Gm32168 NNNTGATCATGAGCACAGAG NGC 2 0.017 Tier III
43 NC_000083.6 17 20111499 + Vmn2r-ps126 NNNTGATCATGAGCACAGAA NAT 2 0.0 Tier III
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Other clones with same target sequence:

(none)