Construct: sgRNA BRDN0001145349
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTGATCATGAGCACAGAA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CSNK2A1 (1457)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000020.11 | 20 | 495724 | - | CSNK2A1 | NNNTGATCATGAGCACAGAA | NGG | 0 | 1.0 | Tier I |
2 | NC_000015.10 | 15 | 75430378 | - | SIN3A | NNNTGAGCATGAGCACAGAA | NGA | 2 | 0.0408 | Tier I |
3 | NC_000011.10 | 11 | 11352615 | - | CSNK2A3 | NNNTGATCATGAGCACAGAA | NGC | 1 | 0.0222 | Tier I |
4 | NC_000003.12 | 3 | 186332235 | + | DGKG | NNNTAAACATGAGCACAGAA | NGG | 2 | 0.7583 | Tier II |
5 | NC_000015.10 | 15 | 65780207 | - | DENND4A | NNNTGAACAAGAGCACAGAA | NGG | 2 | 0.75 | Tier II |
6 | NC_000002.12 | 2 | 105401828 | + | FHL2 | NNNTGTTCATGAGCAAAGAA | NGG | 2 | 0.7143 | Tier II |
7 | NC_000022.11 | 22 | 20880146 | - | SNAP29 | NNNTGACCATGAGCAAAGAA | NGG | 2 | 0.6875 | Tier II |
8 | NC_000016.10 | 16 | 7661020 | + | RBFOX1 | NNNGGATCATGAGCACAGAA | NGG | 1 | 0.625 | Tier II |
9 | NC_000015.10 | 15 | 29059291 | + | APBA2 | NNNTGATCCTAAGCACAGAA | NGG | 2 | 0.5714 | Tier II |
10 | NC_000008.11 | 8 | 27287371 | + | TRIM35 | NNNTGACCATGGGCACAGAA | NGG | 2 | 0.4965 | Tier II |
11 | NC_000003.12 | 3 | 2509589 | + | CNTN4 | NNNTGATAATGGGCACAGAA | NGG | 2 | 0.4694 | Tier II |
12 | NC_000022.11 | 22 | 37376258 | + | ELFN2 | NNNGGATCATGGGCACAGAA | NGG | 2 | 0.4514 | Tier II |
13 | NC_000023.11 | X | 133411210 | + | GPC4 | NNNTGCTCATGAACACAGAA | NGG | 2 | 0.4196 | Tier II |
14 | NC_000001.11 | 1 | 197703607 | + | DENND1B | NNNTGAACATGAGCACACAA | NGG | 2 | 0.4167 | Tier II |
15 | NC_000006.12 | 6 | 33430473 | - | SYNGAP1 | NNNTGATCCTGACCACAGAA | NGG | 2 | 0.2406 | Tier II |
16 | NC_000020.11 | 20 | 61256528 | - | CDH4 | NNNTGATCAAGAGCACAGCA | NGG | 2 | 0.1773 | Tier II |
17 | NC_000016.10 | 16 | 70395896 | + | ST3GAL2 | NNNGGATCATGAGCCCAGAA | NGG | 2 | 0.1705 | Tier II |
18 | NC_000004.12 | 4 | 174664262 | - | GLRA3 | NNNGGATCATGAGCACAGAA | NAG | 2 | 0.162 | Tier II |
19 | NC_000002.12 | 2 | 140928182 | + | LRP1B | NNNTGAACATGAGCAGAGAA | NGG | 2 | 0.1346 | Tier II |
20 | NC_000010.11 | 10 | 49327386 | + | C10orf71 | NNNTGATCATTTGCACAGAA | NGG | 2 | 0.1282 | Tier II |
21 | NC_000005.10 | 5 | 111935178 | - | NREP | NNNTGATCATGAGCACAGCT | NGG | 2 | 0.1241 | Tier II |
22 | NC_000012.12 | 12 | 119019656 | - | SRRM4 | NNNTGTTCATGAGCAGAGAA | NGG | 2 | 0.1099 | Tier II |
23 | NC_000012.12 | 12 | 119637083 | - | TMEM233 | NNNTGATCATGAGCACGGAT | NGG | 2 | 0.1059 | Tier II |
24 | NC_000002.12 | 2 | 230436438 | - | SP100 | NNNTTATCATGAGAACAGAA | NGG | 2 | 0.105 | Tier II |
25 | NC_000002.12 | 2 | 214313800 | + | SPAG16 | NNNTGACCATGAGCACTGAA | NGG | 2 | 0.0917 | Tier II |
26 | NC_000008.11 | 8 | 4367698 | + | CSMD1 | NNNTGACCATGAGCACTGAA | NGG | 2 | 0.0917 | Tier II |
27 | NC_000007.14 | 7 | 72426372 | - | CALN1 | NNNTGATCATGAGAACAGCA | NGG | 2 | 0.0724 | Tier II |
28 | NC_000007.14 | 7 | 108559839 | + | THAP5 | NNNTGATCATGAGAACAGCA | NGG | 2 | 0.0724 | Tier II |
29 | NC_000019.10 | 19 | 57376218 | + | ZNF547 | NNNTGATCATGAGAACAGCA | NGG | 2 | 0.0724 | Tier II |
30 | NC_000023.11 | X | 84497005 | - | HDX | NNNTGATCATGAGAACAGCA | NGG | 2 | 0.0724 | Tier II |
31 | NC_000006.12 | 6 | 138226205 | - | ARFGEF3 | NNNTGATGATGAGCACAGAA | NCG | 2 | 0.0689 | Tier II |
32 | NC_000015.10 | 15 | 48126175 | - | SLC24A5 | NNNTGATCATGAGCAGAGCA | NGG | 2 | 0.0318 | Tier II |
33 | NC_000008.11 | 8 | 55538732 | - | XKR4 | NNNTGATCATGAGTACAGAA | NTG | 2 | 0.0286 | Tier II |
34 | NC_000011.10 | 11 | 11352615 | - | GALNT18 | NNNTGATCATGAGCACAGAA | NGC | 1 | 0.0222 | Tier II |
35 | NC_000007.14 | 7 | 33377657 | + | BBS9 | NNNTGTTCATGAGCACAGAA | NGT | 2 | 0.0115 | Tier II |
36 | NC_000016.10 | 16 | 68376923 | + | SMPD3 | NNNTGATCATAAGGACAGAA | NGG | 2 | 0.0 | Tier II |
37 | NC_000022.11 | 22 | 48561138 | + | TAFA5 | NNNTGATCATAAGGACAGAA | NGG | 2 | 0.0 | Tier II |
38 | NC_000020.11 | 20 | 59990367 | + | CDH26 | NNNTGATCATGGGGACAGAA | NGG | 2 | 0.0 | Tier II |
39 | NC_000003.12 | 3 | 59277833 | + | CFAP20DC-DT | NNNTTATCAGGAGCACAGAA | NGG | 2 | 0.15 | Tier III |
40 | NC_000012.12 | 12 | 58637081 | + | LOC100506869 | NNNTGATCATTTGCACAGAA | NGG | 2 | 0.1282 | Tier III |
41 | NC_000012.12 | 12 | 58637081 | + | LINC02388 | NNNTGATCATTTGCACAGAA | NGG | 2 | 0.1282 | Tier III |
42 | NC_000005.10 | 5 | 111935178 | - | NREP-AS1 | NNNTGATCATGAGCACAGCT | NGG | 2 | 0.1241 | Tier III |
43 | NC_000012.12 | 12 | 119637083 | - | LOC105370027 | NNNTGATCATGAGCACGGAT | NGG | 2 | 0.1059 | Tier III |
44 | NC_000002.12 | 2 | 13178588 | + | LOC105373436 | NNNTTATCATGAGAACAGAA | NGG | 2 | 0.105 | Tier III |
45 | NC_000002.12 | 2 | 13178588 | + | LOC105373484 | NNNTTATCATGAGAACAGAA | NGG | 2 | 0.105 | Tier III |
46 | NC_000001.11 | 1 | 116489115 | - | LOC112268235 | NNNTGATAATGAGCAGAGAA | NGG | 2 | 0.1 | Tier III |
47 | NC_000008.11 | 8 | 81845954 | + | LINC02235 | NNNTGATCATGAGAACAGCA | NGG | 2 | 0.0724 | Tier III |
48 | NC_000001.11 | 1 | 56245814 | - | LOC124904186 | NNNTGATCATGTGCTCAGAA | NGG | 2 | 0.0667 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 152260838 | + | Csnk2a1 | NNNTGATCATGAGCACAGAA | NGG | 0 | 1.0 | Tier I |
2 | NC_000068.7 | 2 | 21576915 | - | Gpr158 | NNNCGATCATCAGCACAGAA | NGG | 2 | 0.2773 | Tier I |
3 | NC_000076.6 | 10 | 69712855 | + | Ank3 | NNNTGATCATGAGCACAGAA | NGG | 0 | 1.0 | Tier II |
4 | NC_000083.6 | 17 | 64708671 | - | Man2a1 | NNNTGATCATAAACACAGAA | NGG | 2 | 0.9231 | Tier II |
5 | NC_000068.7 | 2 | 41629405 | - | Lrp1b | NNNTGATCAAGAGCAAAGAA | NGG | 2 | 0.8571 | Tier II |
6 | NC_000070.6 | 4 | 65591720 | + | Astn2 | NNNTGGACATGAGCACAGAA | NGG | 2 | 0.625 | Tier II |
7 | NC_000082.6 | 16 | 40567713 | - | Lsamp | NNNTGAAAATGAGCACAGAA | NGG | 2 | 0.5688 | Tier II |
8 | NC_000080.6 | 14 | 63463891 | + | Fam167a | NNNTGCTCATGAGCACAGAA | NGG | 1 | 0.4545 | Tier II |
9 | NC_000068.7 | 2 | 59680776 | - | Tanc1 | NNNGGATCATGAGCACAGAT | NGG | 2 | 0.375 | Tier II |
10 | NC_000072.6 | 6 | 51929804 | - | Skap2 | NNNTAATCATGACCACAGAA | NGG | 2 | 0.3649 | Tier II |
11 | NC_000069.6 | 3 | 99312984 | - | Tbx15 | NNNAGATCATGAGCACAGAG | NGG | 2 | 0.3641 | Tier II |
12 | NC_000068.7 | 2 | 96717074 | + | Lrrc4c | NNNTGATCTTGAGCACAGTA | NGG | 2 | 0.3231 | Tier II |
13 | NC_000069.6 | 3 | 131241435 | - | Hadh | NNNTGCTCGTGAGCACAGAA | NGG | 2 | 0.303 | Tier II |
14 | NC_000084.6 | 18 | 9788206 | - | Colec12 | NNNTGATCATCAGCACATAA | NGG | 2 | 0.2857 | Tier II |
15 | NC_000070.6 | 4 | 129333804 | - | Rbbp4 | NNNTGATCCTCAGCACAGAA | NGG | 2 | 0.2449 | Tier II |
16 | NC_000075.6 | 9 | 122987516 | - | Kif15 | NNNTGATCCTCAGCACAGAA | NGG | 2 | 0.2449 | Tier II |
17 | NC_000067.6 | 1 | 152619608 | + | Rgl1 | NNNTGCTCAGGAGCACAGAA | NGG | 2 | 0.2273 | Tier II |
18 | NC_000068.7 | 2 | 164179678 | + | Wfdc5 | NNNTCATCATGAGCACAGCA | NGG | 2 | 0.1626 | Tier II |
19 | NC_000068.7 | 2 | 24006319 | + | Hnmt | NNNTGATCATGTGCACACAA | NGG | 2 | 0.1587 | Tier II |
20 | NC_000083.6 | 17 | 30194241 | + | Zfand3 | NNNTGACCATGAGCTCAGAA | NGG | 2 | 0.1375 | Tier II |
21 | NC_000067.6 | 1 | 38874804 | - | Chst10 | NNNTGAGCATGAGCACAGCA | NGG | 2 | 0.1217 | Tier II |
22 | NC_000079.6 | 13 | 9466605 | + | Dip2c | NNNTGATCCTGAGCTCAGAA | NGG | 2 | 0.1143 | Tier II |
23 | NC_000068.7 | 2 | 34492919 | + | Mapkap1 | NNNTGATCATGAGATCAGAA | NGG | 2 | 0.07 | Tier II |
24 | NC_000077.6 | 11 | 95362503 | - | Fam117a | NNNTGACCATGAGCACAGAA | NGA | 2 | 0.0477 | Tier II |
25 | NC_000069.6 | 3 | 13567268 | + | Ralyl | NNNTGATCATGAGCTGAGAA | NGG | 2 | 0.0308 | Tier II |
26 | NC_000079.6 | 13 | 96069686 | + | Sv2c | NNNTGATCATCAGCACAGAA | NGA | 2 | 0.0298 | Tier II |
27 | NC_000070.6 | 4 | 109357969 | + | Eps15 | NNNTGGTCATGAGCACAGAA | NTG | 2 | 0.0278 | Tier II |
28 | NC_000072.6 | 6 | 97555840 | - | Frmd4b | NNNCGATCATGAGCACAGAA | NTG | 2 | 0.0252 | Tier II |
29 | NC_000084.6 | 18 | 77424835 | - | Loxhd1 | NNNTGATCATGAGCACAGAT | NTG | 2 | 0.0234 | Tier II |
30 | NC_000078.6 | 12 | 114142335 | - | Igh | NNNTCATCATGAGCACAGAT | NGG | 2 | 0.4714 | Tier III |
31 | NC_000069.6 | 3 | 113189484 | - | Amy2-ps1 | NNNTGATCATGGGCACAGAC | NGG | 2 | 0.1641 | Tier III |
32 | NC_000068.7 | 2 | 164179678 | + | A730032A03Rik | NNNTCATCATGAGCACAGCA | NGG | 2 | 0.1626 | Tier III |
33 | NC_000080.6 | 14 | 52848895 | - | Tcra | NNNTGATCATGGGCAGAGAA | NGG | 2 | 0.1111 | Tier III |
34 | NC_000080.6 | 14 | 53159935 | - | Tcra | NNNTGATCATGGGCAGAGAA | NGG | 2 | 0.1111 | Tier III |
35 | NC_000067.6 | 1 | 41939347 | + | Gm29260 | NNNTGCTCATGAGCACAGAA | NGA | 2 | 0.0316 | Tier III |
36 | NC_000070.6 | 4 | 98192112 | - | Gm12691 | NNNTGATCATGACCACAGAA | NGA | 2 | 0.0292 | Tier III |
37 | NC_000070.6 | 4 | 109357969 | + | Gm52702 | NNNTGGTCATGAGCACAGAA | NTG | 2 | 0.0278 | Tier III |
38 | NC_000067.6 | 1 | 156524736 | + | Csnk2a3 | NNNTGATCATGAGCACAGAA | NGC | 1 | 0.0222 | Tier III |
39 | NC_000070.6 | 4 | 75325006 | + | Gm11255 | NNNTGATCATGAGCACAGAA | NGC | 1 | 0.0222 | Tier III |
40 | NC_000080.6 | 14 | 14197800 | + | Csnk2a1-ps1 | NNNTGATCATGAGCACAGAA | NGC | 1 | 0.0222 | Tier III |
41 | NC_000086.7 | X | 16610761 | + | Csnk2a1-ps | NNNTGATCATGAGCACAGAA | NGC | 1 | 0.0222 | Tier III |
42 | NC_000081.6 | 15 | 74460529 | + | Gm32168 | NNNTGATCATGAGCACAGAG | NGC | 2 | 0.017 | Tier III |
43 | NC_000083.6 | 17 | 20111499 | + | Vmn2r-ps126 | NNNTGATCATGAGCACAGAA | NAT | 2 | 0.0 | Tier III |