Construct: sgRNA BRDN0001145356
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGAGGTGCAGCACATAAGGA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- DLG1 (1739)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 197136579 | - | DLG1 | NNNGGTGCAGCACATAAGGA | NGG | 0 | 1.0 | Tier I |
2 | NC_000005.10 | 5 | 114418344 | - | KCNN2 | NNNAGTCCAGCACATAAGGA | NGG | 2 | 0.6188 | Tier II |
3 | NC_000006.12 | 6 | 123855631 | - | NKAIN2 | NNNTGTGCAGCACATAAGGG | NGG | 2 | 0.2781 | Tier II |
4 | NC_000006.12 | 6 | 64640649 | + | EYS | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
5 | NC_000007.14 | 7 | 99512911 | - | ZKSCAN5 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
6 | NC_000007.14 | 7 | 140766369 | + | BRAF | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
7 | NC_000009.12 | 9 | 122569763 | - | OR1J2 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
8 | NC_000009.12 | 9 | 122569763 | - | OR1L8 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
9 | NC_000011.10 | 11 | 114633547 | - | NXPE4 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
10 | NC_000011.10 | 11 | 114633547 | - | NXPE2 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
11 | NC_000024.10 | Y | 18539463 | - | HSFY1 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
12 | NC_000024.10 | Y | 18780945 | + | HSFY2 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier II |
13 | NC_000002.12 | 2 | 105836631 | - | NCK2 | NNNGGTCCAGCACAGAAGGA | NGG | 2 | 0.0344 | Tier II |
14 | NC_000020.11 | 20 | 5831072 | - | SHLD1 | NNNGGTGAAGCACAGAAGGA | NGG | 2 | 0.0325 | Tier II |
15 | NC_000001.11 | 1 | 112625989 | + | CAPZA1 | NNNGGTGCAGCACATAATGA | NGC | 2 | 0.0148 | Tier II |
16 | NC_000003.12 | 3 | 164989015 | + | SI | NNNGGTGCAGCACACCAGGA | NGG | 2 | 0.0 | Tier II |
17 | NC_000001.11 | 1 | 85187945 | + | SYDE2 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
18 | NC_000001.11 | 1 | 241165710 | + | RGS7 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
19 | NC_000002.12 | 2 | 229431715 | + | DNER | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
20 | NC_000003.12 | 3 | 54808135 | + | CACNA2D3 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
21 | NC_000003.12 | 3 | 58822225 | + | CFAP20DC | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
22 | NC_000004.12 | 4 | 46842485 | - | COX7B2 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
23 | NC_000007.14 | 7 | 122404076 | - | CADPS2 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
24 | NC_000008.11 | 8 | 67606261 | + | CPA6 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
25 | NC_000010.11 | 10 | 5175052 | - | AKR1C8 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
26 | NC_000011.10 | 11 | 87241939 | - | TMEM135 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
27 | NC_000017.11 | 17 | 634480 | - | VPS53 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
28 | NC_000023.11 | X | 21451778 | + | CNKSR2 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier II |
29 | NC_000012.12 | 12 | 53405930 | + | SP1 | NNNGGTGCAGCACATCAGTA | NGG | 2 | 0.0 | Tier II |
30 | NC_000004.12 | 4 | 169441128 | + | NEK1 | NNNGGTGCAGCACATTAGGA | NGG | 1 | 0.0 | Tier II |
31 | NC_000008.11 | 8 | 65842203 | - | PDE7A-DT | NNNGGTGCAGAACACAAGGA | NGG | 2 | 0.2045 | Tier III |
32 | NC_000002.12 | 2 | 208663671 | + | LOC101927960 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier III |
33 | NC_000009.12 | 9 | 21297350 | - | LOC107987053 | NNNGGTGCAGCACACAAGCA | NGG | 2 | 0.1223 | Tier III |
34 | NC_000015.10 | 15 | 89512452 | + | LINC00928 | NNNTGTGCAGGACATAAGGA | NGG | 2 | 0.0909 | Tier III |
35 | NC_000017.11 | 17 | 68656302 | + | LINC01482 | NNNGGTGCAGCACATGAGCA | NGG | 2 | 0.0862 | Tier III |
36 | NC_000001.11 | 1 | 89065645 | + | LOC105378841 | NNNGGTGCAGTACATAAGGA | NAG | 2 | 0.0798 | Tier III |
37 | NC_000001.11 | 1 | 112625989 | + | MRPL53P1 | NNNGGTGCAGCACATAATGA | NGC | 2 | 0.0148 | Tier III |
38 | NC_000011.10 | 11 | 112824464 | + | LOC105369498 | NNNGGTGCAGCAGAGAAGGA | NGG | 2 | 0.0068 | Tier III |
39 | NC_000014.9 | 14 | 29005347 | + | LINC02326 | NNNGGTGCAGCACACCAGGA | NGG | 2 | 0.0 | Tier III |
40 | NC_000014.9 | 14 | 29005347 | + | LOC107984685 | NNNGGTGCAGCACACCAGGA | NGG | 2 | 0.0 | Tier III |
41 | NC_000002.12 | 2 | 58824064 | - | LINC01122 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier III |
42 | NC_000004.12 | 4 | 181093630 | - | LINC00290 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier III |
43 | NC_000008.11 | 8 | 67606261 | + | LOC105375886 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier III |
44 | NC_000009.12 | 9 | 103213966 | + | LINC01492 | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier III |
45 | NC_000017.11 | 17 | 27058343 | - | VN1R71P | NNNGGTGCAGCACATCAGCA | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000082.6 | 16 | 31793604 | + | Dlg1 | NNNGGTGCAGCACACAAGGA | NGG | 1 | 0.2727 | Tier I |
2 | NC_000080.6 | 14 | 60027192 | - | Atp8a2 | NNNAGAGCAGCACATAAGGA | NGG | 2 | 0.78 | Tier II |
3 | NC_000070.6 | 4 | 154134385 | + | Trp73 | NNNAGTGCAGCACATGAGGA | NGG | 2 | 0.1731 | Tier II |
4 | NC_000073.6 | 7 | 64710542 | + | Apba2 | NNNGGGGCAGGACATAAGGA | NGG | 2 | 0.1429 | Tier II |
5 | NC_000072.6 | 6 | 7018412 | - | Sdhaf3 | NNNGGTGCAGCAGATAAGGG | NGG | 2 | 0.1043 | Tier II |
6 | NC_000069.6 | 3 | 157301149 | + | Negr1 | NNNGGTGCAGCAGACAAGGA | NGG | 2 | 0.0372 | Tier II |
7 | NC_000084.6 | 18 | 65096131 | + | Nedd4l | NNNGGTTCAGCACAGAAGGA | NGG | 2 | 0.0286 | Tier II |
8 | NC_000073.6 | 7 | 118759317 | + | Vps35l | NNNGGTGCAGCAGATAAGGA | NGA | 2 | 0.0095 | Tier II |
9 | NC_000080.6 | 14 | 18729117 | - | Ube2e2 | NNNGGTGCAGTACATAAGGA | NGC | 2 | 0.0068 | Tier II |
10 | NC_000067.6 | 1 | 133760848 | + | Atp2b4 | NNNGGTGCACCACATAAGGA | NGT | 2 | 0.0065 | Tier II |
11 | NC_000068.7 | 2 | 180191820 | + | Lama5 | NNNGGTGCAGCCCATAAGGA | NGT | 2 | 0.0042 | Tier II |
12 | NC_000082.6 | 16 | 75442365 | - | Robo2 | NNNGGCGCAGCACATCAGGA | NGG | 2 | 0.0 | Tier II |
13 | NC_000074.6 | 8 | 26434938 | - | Gm31898 | NNNGGGGCAGCACACAAGGA | NGG | 2 | 0.1558 | Tier III |
14 | NC_000068.7 | 2 | 33969505 | - | Gm13404 | NNNGGTGCAGGACAGAAGGA | NGG | 2 | 0.0125 | Tier III |