Construct: sgRNA BRDN0001145356

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAGGTGCAGCACATAAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLG1 (1739)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76619

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 197136579 - DLG1 NNNGGTGCAGCACATAAGGA NGG 0 1.0 Tier I
2 NC_000005.10 5 114418344 - KCNN2 NNNAGTCCAGCACATAAGGA NGG 2 0.6188 Tier II
3 NC_000006.12 6 123855631 - NKAIN2 NNNTGTGCAGCACATAAGGG NGG 2 0.2781 Tier II
4 NC_000006.12 6 64640649 + EYS NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
5 NC_000007.14 7 99512911 - ZKSCAN5 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
6 NC_000007.14 7 140766369 + BRAF NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
7 NC_000009.12 9 122569763 - OR1J2 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
8 NC_000009.12 9 122569763 - OR1L8 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
9 NC_000011.10 11 114633547 - NXPE4 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
10 NC_000011.10 11 114633547 - NXPE2 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
11 NC_000024.10 Y 18539463 - HSFY1 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
12 NC_000024.10 Y 18780945 + HSFY2 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier II
13 NC_000002.12 2 105836631 - NCK2 NNNGGTCCAGCACAGAAGGA NGG 2 0.0344 Tier II
14 NC_000020.11 20 5831072 - SHLD1 NNNGGTGAAGCACAGAAGGA NGG 2 0.0325 Tier II
15 NC_000001.11 1 112625989 + CAPZA1 NNNGGTGCAGCACATAATGA NGC 2 0.0148 Tier II
16 NC_000003.12 3 164989015 + SI NNNGGTGCAGCACACCAGGA NGG 2 0.0 Tier II
17 NC_000001.11 1 85187945 + SYDE2 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
18 NC_000001.11 1 241165710 + RGS7 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
19 NC_000002.12 2 229431715 + DNER NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
20 NC_000003.12 3 54808135 + CACNA2D3 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
21 NC_000003.12 3 58822225 + CFAP20DC NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
22 NC_000004.12 4 46842485 - COX7B2 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
23 NC_000007.14 7 122404076 - CADPS2 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
24 NC_000008.11 8 67606261 + CPA6 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
25 NC_000010.11 10 5175052 - AKR1C8 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
26 NC_000011.10 11 87241939 - TMEM135 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
27 NC_000017.11 17 634480 - VPS53 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
28 NC_000023.11 X 21451778 + CNKSR2 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier II
29 NC_000012.12 12 53405930 + SP1 NNNGGTGCAGCACATCAGTA NGG 2 0.0 Tier II
30 NC_000004.12 4 169441128 + NEK1 NNNGGTGCAGCACATTAGGA NGG 1 0.0 Tier II
31 NC_000008.11 8 65842203 - PDE7A-DT NNNGGTGCAGAACACAAGGA NGG 2 0.2045 Tier III
32 NC_000002.12 2 208663671 + LOC101927960 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier III
33 NC_000009.12 9 21297350 - LOC107987053 NNNGGTGCAGCACACAAGCA NGG 2 0.1223 Tier III
34 NC_000015.10 15 89512452 + LINC00928 NNNTGTGCAGGACATAAGGA NGG 2 0.0909 Tier III
35 NC_000017.11 17 68656302 + LINC01482 NNNGGTGCAGCACATGAGCA NGG 2 0.0862 Tier III
36 NC_000001.11 1 89065645 + LOC105378841 NNNGGTGCAGTACATAAGGA NAG 2 0.0798 Tier III
37 NC_000001.11 1 112625989 + MRPL53P1 NNNGGTGCAGCACATAATGA NGC 2 0.0148 Tier III
38 NC_000011.10 11 112824464 + LOC105369498 NNNGGTGCAGCAGAGAAGGA NGG 2 0.0068 Tier III
39 NC_000014.9 14 29005347 + LINC02326 NNNGGTGCAGCACACCAGGA NGG 2 0.0 Tier III
40 NC_000014.9 14 29005347 + LOC107984685 NNNGGTGCAGCACACCAGGA NGG 2 0.0 Tier III
41 NC_000002.12 2 58824064 - LINC01122 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier III
42 NC_000004.12 4 181093630 - LINC00290 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier III
43 NC_000008.11 8 67606261 + LOC105375886 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier III
44 NC_000009.12 9 103213966 + LINC01492 NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier III
45 NC_000017.11 17 27058343 - VN1R71P NNNGGTGCAGCACATCAGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 31793604 + Dlg1 NNNGGTGCAGCACACAAGGA NGG 1 0.2727 Tier I
2 NC_000080.6 14 60027192 - Atp8a2 NNNAGAGCAGCACATAAGGA NGG 2 0.78 Tier II
3 NC_000070.6 4 154134385 + Trp73 NNNAGTGCAGCACATGAGGA NGG 2 0.1731 Tier II
4 NC_000073.6 7 64710542 + Apba2 NNNGGGGCAGGACATAAGGA NGG 2 0.1429 Tier II
5 NC_000072.6 6 7018412 - Sdhaf3 NNNGGTGCAGCAGATAAGGG NGG 2 0.1043 Tier II
6 NC_000069.6 3 157301149 + Negr1 NNNGGTGCAGCAGACAAGGA NGG 2 0.0372 Tier II
7 NC_000084.6 18 65096131 + Nedd4l NNNGGTTCAGCACAGAAGGA NGG 2 0.0286 Tier II
8 NC_000073.6 7 118759317 + Vps35l NNNGGTGCAGCAGATAAGGA NGA 2 0.0095 Tier II
9 NC_000080.6 14 18729117 - Ube2e2 NNNGGTGCAGTACATAAGGA NGC 2 0.0068 Tier II
10 NC_000067.6 1 133760848 + Atp2b4 NNNGGTGCACCACATAAGGA NGT 2 0.0065 Tier II
11 NC_000068.7 2 180191820 + Lama5 NNNGGTGCAGCCCATAAGGA NGT 2 0.0042 Tier II
12 NC_000082.6 16 75442365 - Robo2 NNNGGCGCAGCACATCAGGA NGG 2 0.0 Tier II
13 NC_000074.6 8 26434938 - Gm31898 NNNGGGGCAGCACACAAGGA NGG 2 0.1558 Tier III
14 NC_000068.7 2 33969505 - Gm13404 NNNGGTGCAGGACAGAAGGA NGG 2 0.0125 Tier III
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Other clones with same target sequence:

(none)