Construct: sgRNA BRDN0001145359
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGCGTGGAGCAATCACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK1 (5058)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76466
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
77358931 |
+ |
PAK1 |
NNNCGTGGAGCAATCACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
39357039 |
+ |
SAMD4B |
NNNCCTGGAGCACTCACTGG |
NGG |
2 |
0.1654 |
Tier I |
| 3 |
NC_000019.10 |
19 |
40242593 |
+ |
AKT2 |
NNNCGTGGAGGAGTCACTGG |
NGG |
2 |
0.163 |
Tier I |
| 4 |
NC_000003.12 |
3 |
49896448 |
- |
MST1R |
NNNCGAGGAGCAATCACAGG |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000010.11 |
10 |
102233736 |
- |
PITX3 |
NNNAGTGGAGCAGTCACTGG |
NGG |
2 |
0.5492 |
Tier II |
| 6 |
NC_000010.11 |
10 |
102233736 |
- |
GBF1 |
NNNAGTGGAGCAGTCACTGG |
NGG |
2 |
0.5492 |
Tier II |
| 7 |
NC_000015.10 |
15 |
42139154 |
+ |
PLA2G4F |
NNNCCTGGAGCAAACACTGG |
NGG |
2 |
0.4864 |
Tier II |
| 8 |
NC_000009.12 |
9 |
39153911 |
- |
CNTNAP3 |
NNNCTTGGAACAATCACTGG |
NGG |
2 |
0.28 |
Tier II |
| 9 |
NC_000022.11 |
22 |
31780437 |
+ |
DEPDC5 |
NNNAGTGGAGGAATCACTGG |
NGG |
2 |
0.2105 |
Tier II |
| 10 |
NC_000010.11 |
10 |
124028749 |
- |
CHST15 |
NNNCGTGGAGCAGTAACTGG |
NGG |
2 |
0.1449 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
97883556 |
- |
Pak1 |
NNNCGTGGAGCAATCACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
75785602 |
- |
Zc3h3 |
NNNCGTCCAGCAATCACTGG |
NGG |
2 |
0.4231 |
Tier I |
| 3 |
NC_000082.6 |
16 |
17867727 |
+ |
Dgcr2 |
NNNCGTGGAGCAGTCACTGG |
NGG |
1 |
0.6522 |
Tier II |
| 4 |
NC_000086.7 |
X |
48439132 |
+ |
Elf4 |
NNNGGAGGAGCAATCACTGG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000073.6 |
7 |
3110134 |
- |
Gm7353 |
NNNCGTCCAGCAATCACTGG |
NGG |
2 |
0.4231 |
Tier III |
Other clones with same target sequence:
(none)
