Construct: sgRNA BRDN0001145361
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTGGAATAGGCTCAAGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TRPM6 (140803)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77335
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
74821831 |
- |
TRPM6 |
NNNGGAATAGGCTCAAGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
74009886 |
+ |
TET3 |
NNNAGAATGGGCTCAAGACA |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000016.10 |
16 |
24116461 |
- |
PRKCB |
NNNGGCATAGGCTCAAGACA |
NGG |
1 |
0.4545 |
Tier II |
| 4 |
NC_000006.12 |
6 |
39719423 |
+ |
KIF6 |
NNNGGAATAGTCTCAAAACA |
NGG |
2 |
0.359 |
Tier II |
| 5 |
NC_000005.10 |
5 |
143412396 |
- |
NR3C1 |
NNNGGGATAGGCTCAAGGCA |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000006.12 |
6 |
123901044 |
- |
NKAIN2 |
NNNGCAATAGGCTAAAGACA |
NGG |
2 |
0.275 |
Tier II |
| 7 |
NC_000001.11 |
1 |
103107435 |
- |
COL11A1 |
NNNGGAATAGGTTCAAGATA |
NGG |
2 |
0.2485 |
Tier II |
| 8 |
NC_000003.12 |
3 |
126796994 |
- |
CHCHD6 |
NNNGGAATAGGCAAAAGACA |
NGG |
2 |
0.2423 |
Tier II |
| 9 |
NC_000005.10 |
5 |
78474337 |
- |
SCAMP1 |
NNNGGAATGGGCTAAAGACA |
NGG |
2 |
0.2333 |
Tier II |
| 10 |
NC_000008.11 |
8 |
17336186 |
- |
MTMR7 |
NNNGGAATAGACTCAAGCCA |
NGG |
2 |
0.1905 |
Tier II |
| 11 |
NC_000019.10 |
19 |
3963982 |
+ |
DAPK3 |
NNNGGAAGAGGCTCAAGACA |
NAG |
2 |
0.1901 |
Tier II |
| 12 |
NC_000001.11 |
1 |
69826906 |
+ |
LRRC7 |
NNNGGAAGAGGCTCTAGACA |
NGG |
2 |
0.1467 |
Tier II |
| 13 |
NC_000009.12 |
9 |
134728244 |
+ |
COL5A1 |
NNNGGAATGGGCTCAGGACA |
NGG |
2 |
0.1282 |
Tier II |
| 14 |
NC_000011.10 |
11 |
128721497 |
- |
FLI1 |
NNNGGAATAGGCTAAGGACA |
NGG |
2 |
0.0673 |
Tier II |
| 15 |
NC_000011.10 |
11 |
99911493 |
- |
CNTN5 |
NNNGGAAAAGGCTCAAGACA |
NGA |
2 |
0.0556 |
Tier II |
| 16 |
NC_000001.11 |
1 |
118905428 |
+ |
TBX15 |
NNNGGAAAAGGCTCATGACA |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000008.11 |
8 |
51772718 |
+ |
PXDNL |
NNNGGAAGAGGCTGAAGACA |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000010.11 |
10 |
96904516 |
+ |
LCOR |
NNNGGAATAGCCTGAAGACA |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000001.11 |
1 |
174183756 |
- |
RABGAP1L |
NNNGGAATAGGCTGAAGACA |
NGA |
2 |
0.0 |
Tier II |
| 20 |
NC_000018.10 |
18 |
10339099 |
+ |
LOC105371986 |
NNNGGAATAGGCTTAGGACA |
NGG |
2 |
0.141 |
Tier III |
| 21 |
NC_000008.11 |
8 |
51772718 |
+ |
LOC105375833 |
NNNGGAAGAGGCTGAAGACA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
18791993 |
+ |
Trpm6 |
NNNGAAACAGGCTCAAGACA |
NGG |
2 |
0.8667 |
Tier I |
| 2 |
NC_000081.6 |
15 |
78565877 |
+ |
Rac2 |
NNNGGAACAGGATCAAGACA |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000085.6 |
19 |
44999364 |
+ |
Sema4g |
NNNGAAATAGGCTCAAGACT |
NGG |
2 |
0.52 |
Tier II |
| 4 |
NC_000069.6 |
3 |
110083095 |
+ |
Ntng1 |
NNNGGAATAGGTTCAAAACA |
NGG |
2 |
0.5026 |
Tier II |
| 5 |
NC_000085.6 |
19 |
23889714 |
- |
Apba1 |
NNNGAAATAGGTTCAAGACA |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000079.6 |
13 |
12196674 |
+ |
Mtr |
NNNGGAATAGCCTCAAAACA |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000073.6 |
7 |
67602408 |
+ |
Lrrc28 |
NNNGGAAAAGGCTCAAGATA |
NGG |
2 |
0.3692 |
Tier II |
| 8 |
NC_000076.6 |
10 |
85954416 |
- |
Rtcb |
NNNGGAAAAGCCTCAAGACA |
NGG |
2 |
0.3429 |
Tier II |
| 9 |
NC_000068.7 |
2 |
84993113 |
+ |
Prg3 |
NNNGGAATAGGCTCCAGCCA |
NGG |
2 |
0.0519 |
Tier II |
| 10 |
NC_000081.6 |
15 |
101799501 |
+ |
Krt73 |
NNNGGAATAGGCTCAAGACC |
NGA |
2 |
0.0158 |
Tier II |
| 11 |
NC_000076.6 |
10 |
56066964 |
+ |
Tbc1d32 |
NNNGGAATAGGATCAAGACA |
NGT |
2 |
0.0115 |
Tier II |
| 12 |
NC_000076.6 |
10 |
116109090 |
+ |
Ptprr |
NNNGGAATAGGCTCTAGACA |
NGC |
2 |
0.0044 |
Tier II |
| 13 |
NC_000075.6 |
9 |
116064078 |
- |
Gadl1 |
NNNGGAATAGGGTGAAGACA |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
