Construct: sgRNA BRDN0001145367
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGATCCCGTGCACGAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKQ (1609)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77378
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
971016 |
- |
DGKQ |
NNNGATCCCGTGCACGAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
5846715 |
- |
SHLD1 |
NNNGATCCCGTCCACGTGTG |
NGG |
2 |
0.0706 |
Tier II |
| 3 |
NC_000002.12 |
2 |
161879659 |
+ |
SLC4A10 |
NNNGATCCTGTGCATGAGTG |
NGG |
2 |
0.0583 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
12468266 |
+ |
Lama3 |
NNNCATCCCGTGCAGGAGTG |
NGG |
2 |
0.0265 |
Tier I |
| 2 |
NC_000074.6 |
8 |
66290655 |
+ |
Marchf1 |
NNNGGTCCCTTGCACGAGTG |
NGG |
2 |
0.585 |
Tier II |
| 3 |
NC_000073.6 |
7 |
144071605 |
- |
Shank2 |
NNNGGTACCGTGCACGAGTG |
NGG |
2 |
0.54 |
Tier II |
| 4 |
NC_000084.6 |
18 |
67777026 |
+ |
Seh1l |
NNNGATCCCGTGCACCAGTG |
NTG |
2 |
0.0 |
Tier II |
| 5 |
NC_000084.6 |
18 |
67777026 |
+ |
4930549G23Rik |
NNNGATCCCGTGCACCAGTG |
NTG |
2 |
0.0 |
Tier III |
| 6 |
NC_000075.6 |
9 |
48975332 |
- |
Gm51673 |
NNNGATCCCTTGCACCAGTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
