Construct: sgRNA BRDN0001145372

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGGTAACTGAAATGTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKL2 (8999)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77589

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 75605590 - CDKL2 NNNGTAACTGAAATGTTCAT NGG 0 1.0 Tier I
2 NC_000011.10 11 43475710 - TTC17 NNNATAACTGAAATGCTCAT NGG 2 0.6 Tier II
3 NC_000009.12 9 94778669 + AOPEP NNNGTAATGGAAATGTTCAT NGG 2 0.5417 Tier II
4 NC_000007.14 7 108063685 + LAMB4 NNNCTAACTGAAATGATCAT NGG 2 0.4813 Tier II
5 NC_000004.12 4 20336800 - SLIT2 NNNGTGACTGAAAAGTTCAT NGG 2 0.4422 Tier II
6 NC_000005.10 5 77737989 - TBCA NNNGTAAATGAAATGTTCAA NGG 2 0.3656 Tier II
7 NC_000011.10 11 120611809 + GRIK4 NNNGTTCCTGAAATGTTCAT NGG 2 0.3125 Tier II
8 NC_000007.14 7 111408570 + IMMP2L NNNGTAACTGAAATGTACAA NGG 2 0.3 Tier II
9 NC_000001.11 1 212006811 + INTS7 NNNGTAACTGAAATGATCAT NAG 2 0.2357 Tier II
10 NC_000002.12 2 233410149 + DGKD NNNGTAGCTGAATTGTTCAT NGG 2 0.2118 Tier II
11 NC_000016.10 16 69941206 + WWP2 NNNGTAACTGGAATCTTCAT NGG 2 0.1773 Tier II
12 NC_000007.14 7 106105239 - SYPL1 NNNGAAACTGAAATGGTCAT NGG 2 0.1731 Tier II
13 NC_000008.11 8 132615821 - DNAAF11 NNNGTAACTGAAATGTTCAA NAG 2 0.1458 Tier II
14 NC_000006.12 6 146694802 - ADGB NNNGTAACTGAAATGTCCAG NGG 2 0.1246 Tier II
15 NC_000007.14 7 86825633 - GRM3 NNNGTAACTGAAATTTCCAT NGG 2 0.1008 Tier II
16 NC_000018.10 18 9491051 + RALBP1 NNNTTAACTGAAATCTTCAT NGG 2 0.0992 Tier II
17 NC_000020.11 20 9234720 + PLCB4 NNNGGAACTGAAATTTTCAT NGG 2 0.0914 Tier II
18 NC_000014.9 14 95902471 + TUNAR NNNGTAAGTGAAATGTTGAT NGG 2 0.0857 Tier II
19 NC_000005.10 5 66824542 - MAST4 NNNGTAACTGAAATGTTCAT NGA 1 0.0694 Tier II
20 NC_000013.11 13 98001196 - IPO5 NNNGTAACTGAAATATTCAT NGA 2 0.0654 Tier II
21 NC_000007.14 7 140861629 + BRAF NNNGTATCTGAAATGTGCAT NGG 2 0.0515 Tier II
22 NC_000010.11 10 38030140 + ZNF33A NNNGGAACTGAAATGTTCAT NTG 2 0.0249 Tier II
23 NC_000023.11 X 9519437 - TBL1X NNNGTAACTGAAATGTACAT NTG 2 0.0208 Tier II
24 NC_000002.12 2 225626143 + NYAP2 NNNGTATCTGAAATGTTCAT NTG 2 0.017 Tier II
25 NC_000002.12 2 163638623 - FIGN NNNGTAACTGAAATGTTCAT NTT 2 0.0 Tier II
26 NC_000004.12 4 85007285 + WDFY3-AS2 NNNGTAACTTAAATATTCAT NGG 2 0.7647 Tier III
27 NC_000001.11 1 160432943 + LOC105371467 NNNGGAATTGAAATGTTCAT NGG 2 0.56 Tier III
28 NC_000008.11 8 37654264 + LOC105379380 NNNGTAACCCAAATGTTCAT NGG 2 0.3692 Tier III
29 NC_000020.11 20 10209256 - SNAP25-AS1 NNNGAAACTGAGATGTTCAT NGG 2 0.3611 Tier III
30 NC_000005.10 5 62067760 + LOC124900610 NNNGTAACTGAAAAGTTCAA NGG 2 0.3482 Tier III
31 NC_000002.12 2 195382118 + LOC105376755 NNNGTAACTGAAATGTTAAT NAG 2 0.1396 Tier III
32 NC_000007.14 7 53405668 - LOC105375282 NNNATAACTGAAATGTTCAT NGA 2 0.0625 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 79496578 + Cfap47 NNNGTACCTGAAATGTTCAT NGT 2 0.0071 Tier I
2 NC_000081.6 15 97045986 - Slc38a4 NNNGTTACTAAAATGTTCAT NGG 2 0.6667 Tier II
3 NC_000070.6 4 28824683 + Epha7 NNNGTGACAGAAATGTTCAT NGG 2 0.6633 Tier II
4 NC_000080.6 14 60517966 - Spata13 NNNGTAGCTGAGATGTTCAT NGG 2 0.5098 Tier II
5 NC_000082.6 16 38316462 + Maats1 NNNGTAACTGAAGTGCTCAT NGG 2 0.4348 Tier II
6 NC_000068.7 2 153002463 + Ttll9 NNNGAAACTGAGATGTTCAT NGG 2 0.3611 Tier II
7 NC_000084.6 18 69452571 - Tcf4 NNNTTAATTGAAATGTTCAT NGG 2 0.3182 Tier II
8 NC_000080.6 14 103276243 - Mycbp2 NNNGTAACTGATATGATCAT NGG 2 0.303 Tier II
9 NC_000068.7 2 113625351 + Fmn1 NNNGTCACTGAAATGTTTAT NGG 2 0.2922 Tier II
10 NC_000082.6 16 46405853 + Nectin3 NNNGTCACTGAAATGTTCTT NGG 2 0.2448 Tier II
11 NC_000076.6 10 11007839 + Grm1 NNNCTATCTGAAATGTTCAT NGG 2 0.2316 Tier II
12 NC_000071.6 5 64930314 - Tlr1 NNNGTAACTGGAATGTTCCT NGG 2 0.1345 Tier II
13 NC_000072.6 6 5291652 + Pon2 NNNGTAACTGAAATGTGCTT NGG 2 0.0633 Tier II
14 NC_000078.6 12 31933037 - Hbp1 NNNGTAACTGAATTGTTCAC NGG 2 0.0273 Tier II
15 NC_000078.6 12 31933037 - Cog5 NNNGTAACTGAATTGTTCAC NGG 2 0.0273 Tier II
16 NC_000084.6 18 51294529 + Prr16 NNNGTATCTGAAATGTTCAT NTG 2 0.017 Tier II
17 NC_000075.6 9 24416811 + Dpy19l1 NNNGTAACTTAAATGTTCAT NGT 2 0.0131 Tier II
18 NC_000081.6 15 51993683 - Gm52217 NNNGTAACTGAAATGTTCTT NGT 2 0.0087 Tier II
19 NC_000075.6 9 112170183 + Arpp21 NNNGAAACTGAAATGTTCAT NGT 2 0.0081 Tier II
20 NC_000067.6 1 155317199 - Xpr1 NNNGTAACTGAAATCTTCAT NGT 2 0.0044 Tier II
21 NC_000079.6 13 112776906 - Gm41067 NNNGTAACAGAAATATTCAT NGG 2 0.8739 Tier III
22 NC_000081.6 15 97045986 - Gm32840 NNNGTTACTAAAATGTTCAT NGG 2 0.6667 Tier III
23 NC_000079.6 13 52958506 + Gm40950 NNNGTAACTAAAATTTTCAT NGG 2 0.1333 Tier III
24 NC_000078.6 12 30574841 - Gm32259 NNNGTAACTGCAATTTTCAT NGG 2 0.0571 Tier III
25 NC_000067.6 1 75979390 - Gm32622 NNNGTAGCTGAAATGTTCAT NTG 2 0.0275 Tier III
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Other clones with same target sequence:

(none)