Construct: sgRNA BRDN0001145374
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATCTCATCGAAAACACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDK1 (5163)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77008
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
172565027 |
+ |
PDK1 |
NNNTCTCATCGAAAACACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
119661598 |
+ |
BAG3 |
NNNTCCCATTGAAAACACAT |
NGG |
2 |
0.8556 |
Tier II |
| 3 |
NC_000005.10 |
5 |
6747518 |
+ |
TENT4A |
NNNTCTCAAGGAAAACACAT |
NGG |
2 |
0.3611 |
Tier II |
| 4 |
NC_000001.11 |
1 |
148750640 |
+ |
NUDT4B |
NNNTCCCATCTAAAACACAT |
NGG |
2 |
0.3497 |
Tier II |
| 5 |
NC_000012.12 |
12 |
93400521 |
+ |
NUDT4 |
NNNTCCCATCTAAAACACAT |
NGG |
2 |
0.3497 |
Tier II |
| 6 |
NC_000002.12 |
2 |
211932159 |
+ |
ERBB4 |
NNNTCTCATCTAGAACACAT |
NGG |
2 |
0.2508 |
Tier II |
| 7 |
NC_000006.12 |
6 |
4816871 |
- |
CDYL |
NNNTCTCAGCTAAAACACAT |
NGG |
2 |
0.2381 |
Tier II |
| 8 |
NC_000001.11 |
1 |
84860036 |
- |
LPAR3 |
NNNTCTCATTGAAAAGACAT |
NGG |
2 |
0.1448 |
Tier II |
| 9 |
NC_000016.10 |
16 |
4859584 |
- |
UBN1 |
NNNTCTCATCTTAAACACAT |
NGG |
2 |
0.1282 |
Tier II |
| 10 |
NC_000001.11 |
1 |
181092243 |
- |
IER5 |
NNNTCTCATCCAAAACACAT |
NAG |
2 |
0.1111 |
Tier II |
| 11 |
NC_000013.11 |
13 |
109037745 |
- |
MYO16 |
NNNTCTCATCTACAACACAT |
NGG |
2 |
0.081 |
Tier II |
| 12 |
NC_000012.12 |
12 |
102153285 |
- |
PARPBP |
NNNTCTCATCCAAAACTCAT |
NGG |
2 |
0.0571 |
Tier II |
| 13 |
NC_000001.11 |
1 |
146299857 |
+ |
NUDT4P2 |
NNNTCCCATCTAAAACACAT |
NGG |
2 |
0.3497 |
Tier III |
| 14 |
NC_000009.12 |
9 |
117823801 |
+ |
LOC105376244 |
NNNTCTCATGGAAAACACTT |
NGG |
2 |
0.2094 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
71883886 |
+ |
Pdk1 |
NNNTCTCATCGAAAGCACAT |
NGG |
1 |
0.65 |
Tier I |
| 2 |
NC_000070.6 |
4 |
49380222 |
+ |
Acnat2 |
NNNTCTCCTCCAAAACACAT |
NGG |
2 |
0.1837 |
Tier I |
| 3 |
NC_000070.6 |
4 |
49447437 |
+ |
Acnat1 |
NNNTCTCCTCCAAAACACAT |
NGG |
2 |
0.1837 |
Tier I |
| 4 |
NC_000074.6 |
8 |
18638636 |
- |
Mcph1 |
NNNTCTCAACAAAAACACAT |
NGG |
2 |
0.9286 |
Tier II |
| 5 |
NC_000076.6 |
10 |
34098313 |
- |
Trappc3l |
NNNCCTCATAGAAAACACAT |
NGG |
2 |
0.5608 |
Tier II |
| 6 |
NC_000068.7 |
2 |
54812240 |
- |
Galnt13 |
NNNTTTCATAGAAAACACAT |
NGG |
2 |
0.5515 |
Tier II |
| 7 |
NC_000077.6 |
11 |
16281122 |
- |
Vstm2a |
NNNTTTCAGCGAAAACACAT |
NGG |
2 |
0.3939 |
Tier II |
| 8 |
NC_000081.6 |
15 |
99816772 |
+ |
Lima1 |
NNNTCTCATTGCAAACACAT |
NGG |
2 |
0.2477 |
Tier II |
| 9 |
NC_000070.6 |
4 |
111405698 |
- |
Agbl4 |
NNNTATCATGGAAAACACAT |
NGG |
2 |
0.2222 |
Tier II |
| 10 |
NC_000082.6 |
16 |
7178713 |
- |
Rbfox1 |
NNNTCCCATCGAAATCACAT |
NGG |
2 |
0.1818 |
Tier II |
| 11 |
NC_000080.6 |
14 |
17357230 |
- |
Gm32121 |
NNNTCTCATGGAAATCACAT |
NGG |
2 |
0.0778 |
Tier III |
Other clones with same target sequence:
(none)
