Construct: sgRNA BRDN0001145375

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGATAAACCCAGATACAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K3 (8491)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76404

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 39280277 - MAP4K3 NNNATAAACCCAGATACAAG NGG 0 1.0 Tier I
2 NC_000004.12 4 164797404 + SMIM31 NNNATAAACACAGATACAAA NGG 2 0.8125 Tier II
3 NC_000009.12 9 127268859 - GARNL3 NNNATAAAACCAGATACAAA NGG 2 0.8036 Tier II
4 NC_000005.10 5 170469758 - KCNIP1 NNNATAGACCCAGATACAAA NGG 2 0.6618 Tier II
5 NC_000011.10 11 64663942 + NRXN2 NNNATAAAGCCAGATACAAA NGG 2 0.5804 Tier II
6 NC_000007.14 7 42048721 + GLI3 NNNAGAAAACCAGATACAAG NGG 2 0.5486 Tier II
7 NC_000017.11 17 51953334 - CA10 NNNATAAACCCAAATACATG NGG 2 0.497 Tier II
8 NC_000008.11 8 108694427 + TMEM74 NNNAAAAACCCAGATACAAA NGG 2 0.4688 Tier II
9 NC_000007.14 7 131296572 - MKLN1 NNNAAAAATCCAGATACAAG NGG 2 0.4375 Tier II
10 NC_000012.12 12 131962287 - EP400 NNNACACACCCAGATACAAG NGG 2 0.4375 Tier II
11 NC_000023.11 X 46999601 + JADE3 NNNATAAACCCAAATAAAAG NGG 2 0.4308 Tier II
12 NC_000003.12 3 54528521 - CACNA2D3 NNNATAAACACAGGTACAAG NGG 2 0.4044 Tier II
13 NC_000002.12 2 10808249 + PDIA6 NNNATAAACCAAGATACATG NGG 2 0.4038 Tier II
14 NC_000007.14 7 127815838 - SND1 NNNAGAAACCCAGAAACAAG NGG 2 0.3705 Tier II
15 NC_000003.12 3 14875152 - FGD5 NNNAGAAACCCAGATACATG NGG 2 0.3446 Tier II
16 NC_000001.11 1 184954506 - NIBAN1 NNNATTAACCCAGATAAAAG NGG 2 0.3333 Tier II
17 NC_000023.11 X 29321491 + IL1RAPL1 NNNATAAACACAGATACAGG NGG 2 0.325 Tier II
18 NC_000002.12 2 61287494 - USP34 NNNGAAAACCCAGATACAAG NGG 2 0.3125 Tier II
19 NC_000007.14 7 140906854 - BRAF NNNATAAACCCAGATACAAG NAG 1 0.2593 Tier II
20 NC_000020.11 20 8359978 + PLCB1 NNNACAAACCCAGATGCAAG NGG 2 0.1923 Tier II
21 NC_000019.10 19 56305351 - ZSCAN5A NNNATAAACCCAGATGCAAA NGG 2 0.1803 Tier II
22 NC_000019.10 19 56305351 - EDDM13 NNNATAAACCCAGATGCAAA NGG 2 0.1803 Tier II
23 NC_000019.10 19 56305351 - LOC124900420 NNNATAAACCCAGATGCAAA NGG 2 0.1803 Tier II
24 NC_000015.10 15 43593595 - CKMT1B NNNATAAACACAGATACCAG NGG 2 0.1651 Tier II
25 NC_000015.10 15 43693427 - CKMT1A NNNATAAACACAGATACCAG NGG 2 0.1651 Tier II
26 NC_000001.11 1 66772088 - DYNLT5 NNNATAAACCCAGAAACAAG NAG 2 0.1501 Tier II
27 NC_000011.10 11 77226147 - GDPD4 NNNATAAACCTAGATAAAAG NGG 2 0.1436 Tier II
28 NC_000004.12 4 42484586 + ATP8A1 NNNATAAACCCAGACAAAAG NGG 2 0.1273 Tier II
29 NC_000001.11 1 179608319 - TDRD5 NNNATAAACCCACATACAAG NAG 2 0.1092 Tier II
30 NC_000006.12 6 135484468 - AHI1 NNNATAAAACCAGATACAAG NGA 2 0.0595 Tier II
31 NC_000016.10 16 6972838 + RBFOX1 NNNATAAAACCAGAGACAAG NGG 2 0.0429 Tier II
32 NC_000006.12 6 110192247 + CDC40 NNNAAAAACCCAGATACAAG NTG 2 0.0195 Tier II
33 NC_000016.10 16 73854838 - ZFHX3 NNNAAAAACCCAGATACAAG NGC 2 0.0111 Tier II
34 NC_000003.12 3 14448652 + SLC6A6 NNNACAAACCCAGATTCAAG NGG 2 0.0 Tier II
35 NC_000005.10 5 133367950 + FSTL4 NNNATAAACCCAGATCCAAC NGG 2 0.0 Tier II
36 NC_000007.14 7 95692023 - LOC105375409 NNNATAAATCCAGTTACAAG NGG 2 0.4667 Tier III
37 NC_000004.12 4 34912780 + LOC107986272 NNNAGACACCCAGATACAAG NGG 2 0.28 Tier III
38 NC_000003.12 3 178307588 + LOC105374235 NNNATAAACTCAGATACAAG NGA 2 0.0654 Tier III
39 NC_000005.10 5 133367950 + LOC124901068 NNNATAAACCCAGATCCAAC NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 80612036 - Map4k3 NNNATAAACCCAGACACGAG NGG 2 0.1091 Tier I
2 NC_000084.6 18 63748081 - Wdr7 NNNATGAATCCAGATACAAG NGG 2 0.625 Tier II
3 NC_000072.6 6 93833434 + Magi1 NNNAGAAACCCAGATACAAA NGG 2 0.6 Tier II
4 NC_000067.6 1 187256796 - Gpatch2 NNNAGAGACCCAGATACAAG NGG 2 0.4518 Tier II
5 NC_000085.6 19 31039810 + Prkg1 NNNGTAAACCCGGATACAAG NGG 2 0.4514 Tier II
6 NC_000069.6 3 96762259 - Rnf115 NNNAAAAACACAGATACAAG NGG 2 0.4333 Tier II
7 NC_000078.6 12 61794468 + Lrfn5 NNNATTAACCCAGAAACAAG NGG 2 0.4135 Tier II
8 NC_000078.6 12 86750588 - Lrrc74a NNNAGAAAGCCAGATACAAG NGG 2 0.3962 Tier II
9 NC_000068.7 2 150910427 - Gins1 NNNATGAACCCAGATACATG NGG 2 0.3846 Tier II
10 NC_000068.7 2 130495915 + Ptpra NNNAAAAAGCCAGATACAAG NGG 2 0.3095 Tier II
11 NC_000086.7 X 160404448 - Adgrg2 NNNAAAAACCCAGAAACAAG NGG 2 0.2895 Tier II
12 NC_000080.6 14 101447765 + Tbc1d4 NNNATAAACCATGATACAAG NGG 2 0.25 Tier II
13 NC_000073.6 7 67651029 - Ttc23 NNNATAAACCCAAATACAAG NAG 2 0.2393 Tier II
14 NC_000075.6 9 107451477 - Cacna2d2 NNNATAAACCCAGATAATAG NGG 2 0.2333 Tier II
15 NC_000077.6 11 87657814 + Hsf5 NNNATAAACCAAGACACAAG NGG 2 0.2045 Tier II
16 NC_000069.6 3 101053676 + Ptgfrn NNNATAAACACAGATGCAAG NGG 2 0.1667 Tier II
17 NC_000070.6 4 77304035 + Ptprd NNNATAAACCCAGACAAAAG NGG 2 0.1273 Tier II
18 NC_000072.6 6 118891892 + Cacna1c NNNATAAACCCAGATACCTG NGG 2 0.1026 Tier II
19 NC_000072.6 6 144142663 - Sox5 NNNATAAACGCCGATACAAG NGG 2 0.1023 Tier II
20 NC_000068.7 2 75483667 - Gm50595 NNNAGAAACCCAGATACAAG NGA 2 0.0444 Tier II
21 NC_000086.7 X 69865856 + Aff2 NNNAGAAACCCAGATACAAG NTG 2 0.0249 Tier II
22 NC_000070.6 4 109152075 + Osbpl9 NNNATAAACACAGATACAAG NGC 2 0.0193 Tier II
23 NC_000076.6 10 10858283 + Grm1 NNNATAGACCCAGATACAAG NGC 2 0.0157 Tier II
24 NC_000068.7 2 92001486 - Creb3l1 NNNATAAACCCAGAGACAAG NAG 2 0.013 Tier II
25 NC_000068.7 2 73692091 + Chn1 NNNATAAACCCAGATAAAAG NGT 2 0.0075 Tier II
26 NC_000078.6 12 102374492 + Rin3 NNNATAAACCCACATTCAAG NGG 2 0.0 Tier II
27 NC_000080.6 14 47003163 + Samd4 NNNATAAACCCAGATTCCAG NGG 2 0.0 Tier II
28 NC_000070.6 4 109152075 + Gm52703 NNNATAAACACAGATACAAG NGC 2 0.0193 Tier III
29 NC_000080.6 14 47003163 + Gm52121 NNNATAAACCCAGATTCCAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)