Construct: sgRNA BRDN0001145383
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCTGGCTGAGATGCTTACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK4 (5596)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77221
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
50715202 |
+ |
MAPK4 |
NNNTGGCTGAGATGCTTACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
134621143 |
+ |
ZFAT |
NNNTGTCTGAGATGCTTAAG |
NGG |
2 |
0.2857 |
Tier II |
| 3 |
NC_000009.12 |
9 |
16794184 |
- |
BNC2 |
NNNTGGCTGAGATGCTTACA |
NAG |
2 |
0.2431 |
Tier II |
| 4 |
NC_000002.12 |
2 |
43976090 |
- |
LRPPRC |
NNNTGGCTGAGATGCTAAAG |
NGG |
2 |
0.2286 |
Tier II |
| 5 |
NC_000006.12 |
6 |
621494 |
- |
EXOC2 |
NNNTGGCTGAGATGCTCACG |
NAG |
2 |
0.183 |
Tier II |
| 6 |
NC_000006.12 |
6 |
143564636 |
+ |
PHACTR2 |
NNNTGGCTGAGCTGCTGACG |
NGG |
2 |
0.031 |
Tier II |
| 7 |
NC_000003.12 |
3 |
59765061 |
+ |
FHIT |
NNNTGGCTGAGATGCTTAAG |
NTG |
2 |
0.0167 |
Tier II |
| 8 |
NC_000019.10 |
19 |
44110397 |
- |
ZNF225-AS1 |
NNNTGGCAGAGAAGCTTACG |
NGG |
2 |
0.5538 |
Tier III |
| 9 |
NC_000009.12 |
9 |
106433611 |
+ |
LOC107987108 |
NNNTGGCTGAGATGCTCATG |
NGG |
2 |
0.3258 |
Tier III |
| 10 |
NC_000006.12 |
6 |
143564636 |
+ |
PHACTR2-AS1 |
NNNTGGCTGAGCTGCTGACG |
NGG |
2 |
0.031 |
Tier III |
| 11 |
NC_000003.12 |
3 |
59765061 |
+ |
CFAP20DC-DT |
NNNTGGCTGAGATGCTTAAG |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
73937152 |
- |
Mapk4 |
NNNTGGCAGAGATGCTCACG |
NGG |
2 |
0.5647 |
Tier I |
| 2 |
NC_000072.6 |
6 |
32447085 |
- |
Plxna4 |
NNNTGGCTGAGATGCATACC |
NGG |
2 |
0.3896 |
Tier II |
| 3 |
NC_000070.6 |
4 |
103187998 |
+ |
Slc35d1 |
NNNAGGCTGAGATCCTTACG |
NGG |
2 |
0.2041 |
Tier II |
| 4 |
NC_000079.6 |
13 |
32798732 |
+ |
Mylk4 |
NNNTGGCTGAGATGCTTGCC |
NGG |
2 |
0.1714 |
Tier II |
| 5 |
NC_000068.7 |
2 |
101833175 |
+ |
Prr5l |
NNNTGGCTGAGCTGCTAACG |
NGG |
2 |
0.1404 |
Tier II |
| 6 |
NC_000080.6 |
14 |
101905971 |
- |
Lmo7 |
NNNTGGCTGAGATGATTAAG |
NGG |
2 |
0.0952 |
Tier II |
| 7 |
NC_000070.6 |
4 |
141453320 |
- |
Zbtb17 |
NNNTGGCTGAGATGCTTCCC |
NGG |
2 |
0.0816 |
Tier II |
| 8 |
NC_000068.7 |
2 |
11692706 |
- |
Il2ra |
NNNTGGCTGAGATGCTCACG |
NGA |
2 |
0.049 |
Tier II |
| 9 |
NC_000068.7 |
2 |
11692706 |
- |
Gm39760 |
NNNTGGCTGAGATGCTCACG |
NGA |
2 |
0.049 |
Tier III |
| 10 |
NC_000068.7 |
2 |
31543724 |
+ |
Gm32952 |
NNNTGCCTGAGATGCTTACG |
NGA |
2 |
0.0473 |
Tier III |
| 11 |
NC_000083.6 |
17 |
4622092 |
+ |
Gm34259 |
NNNTGGCTGAGATGCTTACT |
NGC |
2 |
0.0156 |
Tier III |
Other clones with same target sequence:
(none)
