Construct: sgRNA BRDN0001145388
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCGGACGAGGAACATACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CRKL (1399)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77508
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
20918036 |
- |
CRKL |
NNNGGACGAGGAACATACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
103389626 |
- |
C12orf42 |
NNNGGAAGAGGAACATACAG |
NGG |
2 |
0.3214 |
Tier II |
| 3 |
NC_000020.11 |
20 |
59735623 |
+ |
PHACTR3 |
NNNGGACGAGGAACAGACCC |
NGG |
2 |
0.1484 |
Tier II |
| 4 |
NC_000002.12 |
2 |
111018261 |
+ |
ACOXL |
NNNGGAGGAGGCACATACCG |
NGG |
2 |
0.1238 |
Tier II |
| 5 |
NC_000015.10 |
15 |
66669572 |
+ |
LINC01169 |
NNNGGACGGGGAGCATACCG |
NGG |
2 |
0.4348 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
70190719 |
+ |
Ccdc6 |
NNNGGACAAGGAACATACCT |
NGG |
2 |
0.7 |
Tier II |
| 2 |
NC_000077.6 |
11 |
33733983 |
- |
Kcnip1 |
NNNGGACGAGGAACCAACCG |
NGG |
2 |
0.2479 |
Tier II |
| 3 |
NC_000071.6 |
5 |
121875060 |
- |
Cux2 |
NNNGGACGAGGAACTCACCG |
NGG |
2 |
0.1333 |
Tier II |
| 4 |
NC_000073.6 |
7 |
118815804 |
- |
Vps35l |
NNNGGAAGAGGAACATGCCG |
NGG |
2 |
0.1324 |
Tier II |
| 5 |
NC_000071.6 |
5 |
121875060 |
- |
Gm52785 |
NNNGGACGAGGAACTCACCG |
NGG |
2 |
0.1333 |
Tier III |
Other clones with same target sequence:
(none)
