Construct: sgRNA BRDN0001145390
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTAAAAAAGAGCATCCTCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NIM1K (167359)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77271
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
43280270 |
+ |
NIM1K |
NNNAAAAAGAGCATCCTCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
4429605 |
+ |
SUMF1 |
NNNAAAAAGAGCATCCTCAA |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000023.11 |
X |
64354206 |
+ |
MTMR8 |
NNNAAAAAGAGGATCCTCAA |
NGG |
2 |
0.3175 |
Tier II |
| 4 |
NC_000003.12 |
3 |
44565446 |
- |
ZKSCAN7 |
NNNCAAAAGAGCATCCTTGA |
NGG |
2 |
0.2269 |
Tier II |
| 5 |
NC_000010.11 |
10 |
7410209 |
+ |
SFMBT2 |
NNNAAAAAGACCATCCTCGC |
NGG |
2 |
0.0974 |
Tier II |
| 6 |
NC_000002.12 |
2 |
124187158 |
+ |
CNTNAP5 |
NNNAAAAAGAGCAACCTGGA |
NGG |
2 |
0.0825 |
Tier II |
| 7 |
NC_000004.12 |
4 |
158253804 |
- |
TMEM144 |
NNNAAAAAGAGCATCCTCGT |
NTG |
2 |
0.0234 |
Tier II |
| 8 |
NC_000005.10 |
5 |
161835774 |
+ |
LOC105377696 |
NNNAGAAAGAGCATCCTTGA |
NGG |
2 |
0.4629 |
Tier III |
| 9 |
NC_000005.10 |
5 |
88685909 |
+ |
MIR9-2HG |
NNNAACAAGAGCATCCACGA |
NGG |
2 |
0.2424 |
Tier III |
| 10 |
NC_000005.10 |
5 |
88685909 |
+ |
MEF2C-AS2 |
NNNAACAAGAGCATCCACGA |
NGG |
2 |
0.2424 |
Tier III |
| 11 |
NC_000003.12 |
3 |
44565446 |
- |
ZKSCAN7-AS1 |
NNNCAAAAGAGCATCCTTGA |
NGG |
2 |
0.2269 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
119712506 |
- |
Nim1k |
NNNAAGAAGAGCATCCTCGA |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000078.6 |
12 |
89892246 |
- |
Nrxn3 |
NNNAAAAAGAGAAACCTCGA |
NGG |
2 |
0.4422 |
Tier II |
| 3 |
NC_000079.6 |
13 |
114124676 |
+ |
Arl15 |
NNNAAAAAGAGCATCCTTGT |
NGG |
2 |
0.3857 |
Tier II |
| 4 |
NC_000069.6 |
3 |
66147583 |
- |
Veph1 |
NNNGAAAAGAGCATCCTCCA |
NGG |
2 |
0.2802 |
Tier II |
| 5 |
NC_000084.6 |
18 |
43437697 |
- |
Dpysl3 |
NNNAAAAAGGGCATCCTCCA |
NGG |
2 |
0.249 |
Tier II |
| 6 |
NC_000071.6 |
5 |
20487487 |
+ |
Magi2 |
NNNAACAAGAGCATCCTCCA |
NGG |
2 |
0.2038 |
Tier II |
| 7 |
NC_000071.6 |
5 |
134291607 |
+ |
Gtf2i |
NNNTAAAAGAGCATCCTCGC |
NGG |
2 |
0.1446 |
Tier II |
| 8 |
NC_000069.6 |
3 |
87152981 |
+ |
Kirrel |
NNNAAAAAGAGGATCCTGGA |
NGG |
2 |
0.0593 |
Tier II |
| 9 |
NC_000074.6 |
8 |
16423720 |
+ |
Csmd1 |
NNNAAAATGAGCATCCTGGA |
NGG |
2 |
0.0571 |
Tier II |
| 10 |
NC_000067.6 |
1 |
21473580 |
- |
Kcnq5 |
NNNCAAAAGAGCATCCTGGA |
NGG |
2 |
0.0471 |
Tier II |
| 11 |
NC_000082.6 |
16 |
42312893 |
+ |
Gap43 |
NNNAAAAAGAGCATCCTCGT |
NTG |
2 |
0.0234 |
Tier II |
| 12 |
NC_000083.6 |
17 |
10245194 |
+ |
Qk |
NNNAAAAAGAGCAACCTCGA |
NGC |
2 |
0.0138 |
Tier II |
| 13 |
NC_000079.6 |
13 |
114124676 |
+ |
A430090L17Rik |
NNNAAAAAGAGCATCCTTGT |
NGG |
2 |
0.3857 |
Tier III |
| 14 |
NC_000076.6 |
10 |
97749671 |
+ |
4930525C09Rik |
NNNAAGAAGAGCATCCTAGA |
NGG |
2 |
0.3846 |
Tier III |
| 15 |
NC_000076.6 |
10 |
97749671 |
+ |
Gm10754 |
NNNAAGAAGAGCATCCTAGA |
NGG |
2 |
0.3846 |
Tier III |
Other clones with same target sequence:
(none)
