Construct: sgRNA BRDN0001145392

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGAGTACGTGACTGTACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STRADA (92335)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76055

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 63713457 - STRADA NNNAGTACGTGACTGTACGG NGG 0 1.0 Tier I
2 NC_000015.10 15 51950737 + LEO1 NNNAGTATGTGGCTGTACGG NGG 2 0.6319 Tier II
3 NC_000003.12 3 189827371 - TP63 NNNAGTACGTGTCTGTAAGG NGG 2 0.1795 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 60111947 + Rai1 NNNAGTATGTGACTTTACGG NGG 2 0.125 Tier II
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Other clones with same target sequence:

(none)