Construct: sgRNA BRDN0001145394
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGACAGGGTACGAGTATCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK32B (55351)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76374
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
5446713 |
- |
STK32B |
NNNCAGGGTACGAGTATCCG |
NGG |
0 |
1.0 |
Tier I |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
3919647 |
+ |
Aldh3b1 |
NNNCAAGGTAAGAGTATCCG |
NGG |
2 |
0.75 |
Tier II |
| 2 |
NC_000076.6 |
10 |
83116062 |
+ |
Chst11 |
NNNCAAGGTACGAGGATCCG |
NGG |
2 |
0.05 |
Tier II |
| 3 |
NC_000070.6 |
4 |
70351712 |
- |
Cdk5rap2 |
NNNCAGGGTACGAGTAGCCG |
NAG |
2 |
0.0305 |
Tier II |
| 4 |
NC_000069.6 |
3 |
49751626 |
+ |
Pcdh18 |
NNNGAGGGTACGAGGATCCG |
NGG |
2 |
0.025 |
Tier II |
| 5 |
NC_000085.6 |
19 |
3919647 |
+ |
Gm42059 |
NNNCAAGGTAAGAGTATCCG |
NGG |
2 |
0.75 |
Tier III |
| 6 |
NC_000076.6 |
10 |
83116062 |
+ |
LOC115487465 |
NNNCAAGGTACGAGGATCCG |
NGG |
2 |
0.05 |
Tier III |
Other clones with same target sequence:
(none)
