Construct: sgRNA BRDN0001145395
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGATCCTCGACAAACAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKACA (5566)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75516
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
14106765 |
- |
PRKACA |
NNNGATCCTCGACAAACAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
69013861 |
- |
PRKACG |
NNNGATCCTCAACAAGCAGA |
NGG |
2 |
0.1923 |
Tier I |
| 3 |
NC_000018.10 |
18 |
79681146 |
- |
CTDP1 |
NNNGATCCTCAACAAACACA |
NGG |
2 |
0.4483 |
Tier II |
| 4 |
NC_000003.12 |
3 |
121568713 |
- |
ARGFX |
NNNGATCCTCGACAAACACA |
NGG |
1 |
0.4483 |
Tier II |
| 5 |
NC_000001.11 |
1 |
167053091 |
+ |
GPA33 |
NNNAATGCTCGACAAACAGA |
NGG |
2 |
0.4235 |
Tier II |
| 6 |
NC_000009.12 |
9 |
100016049 |
+ |
ERP44 |
NNNGATCCTCAACCAACAGA |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000017.11 |
17 |
18019862 |
- |
ATPAF2 |
NNNGATCCTCTACAAACAGA |
NGA |
2 |
0.0267 |
Tier II |
| 8 |
NC_000001.11 |
1 |
112357225 |
- |
LINC02884 |
NNNGATCCTAGACAGACAGA |
NGG |
2 |
0.5633 |
Tier III |
| 9 |
NC_000001.11 |
1 |
167053091 |
+ |
LOC105371600 |
NNNAATGCTCGACAAACAGA |
NGG |
2 |
0.4235 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
88108437 |
+ |
Iqgap3 |
NNNGATCCTGGACAAACAGA |
NGG |
1 |
0.3889 |
Tier I |
| 2 |
NC_000084.6 |
18 |
57381683 |
+ |
Prrc1 |
NNNGATCCACGAGAAACAGA |
NGG |
2 |
0.1266 |
Tier I |
| 3 |
NC_000073.6 |
7 |
19863711 |
- |
Ceacam16 |
NNNGATCCTCAACAAACACA |
NGG |
2 |
0.4483 |
Tier II |
| 4 |
NC_000071.6 |
5 |
143897582 |
- |
Eif2ak1 |
NNNGATCCTTGACAAACCGA |
NGG |
2 |
0.1793 |
Tier II |
| 5 |
NC_000084.6 |
18 |
22776082 |
- |
Nol4 |
NNNGATCCTCTACAAAGAGA |
NGG |
2 |
0.0226 |
Tier II |
Other clones with same target sequence:
(none)
