Construct: sgRNA BRDN0001145401

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGAACATCAAGGCCAGCAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK4 (205)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77694

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 65148548 + AK4 NNNAACATCAAGGCCAGCAC NGG 0 1.0 Tier I
2 NC_000004.12 4 7042847 - CCDC96 NNNAAGATCAAGGCCAGCTC NGG 2 0.2692 Tier I
3 NC_000020.11 20 42352140 - PTPRT NNNACCATCAAGGCCAGCAC NGC 2 0.0111 Tier I
4 NC_000002.12 2 166372975 + SCN9A NNNAACATCAAGACCAACAC NGG 2 0.8615 Tier II
5 NC_000012.12 12 86155856 - MGAT4C NNNAAAGTCAAGGCCAGCAC NGG 2 0.6555 Tier II
6 NC_000022.11 22 41812812 + CCDC134 NNNAACACCAGGGCCAGCAC NGG 2 0.65 Tier II
7 NC_000004.12 4 7270416 - SORCS2 NNNAACAGAAAGGCCAGCAC NGG 2 0.6286 Tier II
8 NC_000011.10 11 74942478 + XRRA1 NNNAATATCAGGGCCAGCAC NGG 2 0.6036 Tier II
9 NC_000007.14 7 80884913 + SEMA3C NNNAACATTAAGGCCAGTAC NGG 2 0.5625 Tier II
10 NC_000011.10 11 95803806 - CEP57 NNNAACATCAAAGCCAGAAC NGG 2 0.5026 Tier II
11 NC_000017.11 17 20313841 - SPECC1 NNNAACGTGAAGGCCAGCAC NGG 2 0.437 Tier II
12 NC_000018.10 18 34514962 - DTNA NNNAATTTCAAGGCCAGCAC NGG 2 0.4062 Tier II
13 NC_000018.10 18 49916308 + MYO5B NNNGACATCAAGGCCAGTAC NGG 2 0.4018 Tier II
14 NC_000019.10 19 30656180 - ZNF536 NNNAACACCAAGGACAGCAC NGG 2 0.35 Tier II
15 NC_000018.10 18 50821814 + MRO NNNAACATCAGGGCCAGAAC NGG 2 0.35 Tier II
16 NC_000013.11 13 36046839 + DCLK1 NNNCACATAAAGGCCAGCAC NGG 2 0.3025 Tier II
17 NC_000022.11 22 33432948 - LARGE1 NNNAACATCAACGCCAGCTC NGG 2 0.2851 Tier II
18 NC_000020.11 20 14567612 - MACROD2 NNNAACAACAAGGACAGCAC NGG 2 0.28 Tier II
19 NC_000007.14 7 151699478 + PRKAG2 NNNATCATGAAGGCCAGCAC NGG 2 0.2251 Tier II
20 NC_000002.12 2 174751342 - CHRNA1 NNNAACAGCAAGGCCAGCCC NGG 2 0.1517 Tier II
21 NC_000010.11 10 69910896 - COL13A1 NNNAACATCCAGGCCACCAC NGG 2 0.0784 Tier II
22 NC_000017.11 17 31345659 + NF1 NNNAACATCAAGGCCAGCAC NGA 1 0.0694 Tier II
23 NC_000012.12 12 109722265 - FAM222A NNNAACATCCAGGCCAGCCC NGG 2 0.069 Tier II
24 NC_000002.12 2 141823360 + LRP1B NNNAACATGAAGGCCAGCAC NGT 2 0.01 Tier II
25 NC_000021.9 21 46570751 - DIP2A NNNAACATGAAGGCCAGCAC NGT 2 0.01 Tier II
26 NC_000015.10 15 77146898 + PEAK1 NNNAACATCAAGGCCAGCAA NGT 2 0.0081 Tier II
27 NC_000016.10 16 85540282 - GSE1 NNNAACATCAAGGCCTGAAC NGG 2 0.0 Tier II
28 NC_000002.12 2 230915284 - GPR55 NNNAACATCAATGGCAGCAC NGG 2 0.0 Tier II
29 NC_000014.9 14 101122789 + LINC02285 NNNTAGATCAAGGCCAGCAC NGG 2 0.3182 Tier III
30 NC_000020.11 20 14567612 - MACROD2-IT1 NNNAACAACAAGGACAGCAC NGG 2 0.28 Tier III
31 NC_000013.11 13 112184512 - LOC105370370 NNNAACAGCAAGGCCAGCGC NGG 2 0.275 Tier III
32 NC_000004.12 4 7042847 - LOC100129931 NNNAAGATCAAGGCCAGCTC NGG 2 0.2692 Tier III
33 NC_000006.12 6 80078225 - AK4P5 NNNAACATCAAGGCCAACAC NAG 2 0.242 Tier III
34 NC_000004.12 4 119403974 - LOC100131884 NNNATGATCAAGGCCAGCAC NGG 2 0.1818 Tier III
35 NC_000004.12 4 57120260 - IGFBP7-AS1 NNNAACATCAAGGAAAGCAC NGG 2 0.0778 Tier III
36 NC_000012.12 12 31616300 - AK4P3 NNNAACATCAAGGCCAGCAC NGA 1 0.0694 Tier III
37 NC_000017.11 17 31345659 + AK4P1 NNNAACATCAAGGCCAGCAC NGA 1 0.0694 Tier III
38 NC_000007.14 7 112980856 + HRAT17 NNNAACAACAAGGCCAGCAC NGA 2 0.0556 Tier III
39 NC_000005.10 5 149427149 + CARMN NNNAACATCAAGGCCACCAC NGT 2 0.0038 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 60944929 + Mmrn1 NNNAGCATCAAGCCCAGCAC NGG 2 0.3032 Tier I
2 NC_000081.6 15 79690949 - Gtpbp1 NNNAACATCGAGGCCGGCAC NGG 2 0.1068 Tier I
3 NC_000068.7 2 161811991 - Ptprt NNNACCATCAAGGCCAGCAC NGC 2 0.0111 Tier I
4 NC_000072.6 6 115894311 + Ift122 NNNAACATCAAGGCCAGCAC NTT 2 0.0 Tier I
5 NC_000072.6 6 114782673 - Atg7 NNNAAAACCAAGGCCAGCAC NGG 2 0.9286 Tier II
6 NC_000078.6 12 101561716 + Catsperb NNNAACATAAAGGCCAGCAC NGG 1 0.8571 Tier II
7 NC_000084.6 18 21169560 + Garem1 NNNAACATTAAAGCCAGCAC NGG 2 0.8167 Tier II
8 NC_000082.6 16 84990917 + App NNNAACATCAAGATCAGCAC NGG 2 0.6769 Tier II
9 NC_000068.7 2 16136882 + Malrd1 NNNTACATCAAAGCCAGCAC NGG 2 0.5939 Tier II
10 NC_000071.6 5 71823201 - Gabrb1 NNNAAAATGAAGGCCAGCAC NGG 2 0.5748 Tier II
11 NC_000070.6 4 155389601 - Gabrd NNNAACAACAGGGCCAGCAC NGG 2 0.52 Tier II
12 NC_000083.6 17 56093037 + Hdgfl2 NNNAACTTCAAGGCCAGCAC NGG 1 0.4375 Tier II
13 NC_000070.6 4 43073218 - Unc13b NNNAAATTCAAGGCCAGCAC NGG 2 0.4062 Tier II
14 NC_000070.6 4 83723295 - Ccdc171 NNNAAATTCAAGGCCAGCAC NGG 2 0.4062 Tier II
15 NC_000079.6 13 51681641 - Secisbp2 NNNACCAGCAAGGCCAGCAC NGG 2 0.3667 Tier II
16 NC_000077.6 11 101246648 + Ramp2 NNNGACATCGAGGCCAGCAC NGG 2 0.3472 Tier II
17 NC_000069.6 3 29492364 - Egfem1 NNNAACATAAATGCCAGCAC NGG 2 0.3297 Tier II
18 NC_000075.6 9 116135121 + Tgfbr2 NNNGAGATCAAGGCCAGCAC NGG 2 0.3125 Tier II
19 NC_000072.6 6 56087673 - Pde1c NNNATCAGCAAGGCCAGCAC NGG 2 0.2667 Tier II
20 NC_000067.6 1 132911850 - Lrrn2 NNNAACATCAAAGCCAGCAC NAG 2 0.242 Tier II
21 NC_000076.6 10 122793366 - Ppm1h NNNAACATCAAAGCCAGCAC NAG 2 0.242 Tier II
22 NC_000068.7 2 6764529 + Celf2 NNNAAAATCAAGGCCATCAC NGG 2 0.2321 Tier II
23 NC_000077.6 11 23608687 + 0610010F05Rik NNNAAGCTCAAGGCCAGCAC NGG 2 0.2188 Tier II
24 NC_000069.6 3 65540267 + Tiparp NNNAAGTTCAAGGCCAGCAC NGG 2 0.2188 Tier II
25 NC_000071.6 5 140904255 - Card11 NNNAAGTTCAAGGCCAGCAC NGG 2 0.2188 Tier II
26 NC_000074.6 8 122970588 + Ankrd11 NNNAAGTTCAAGGCCAGCAC NGG 2 0.2188 Tier II
27 NC_000083.6 17 66810272 + Ptprm NNNAACATGCAGGCCAGCAC NGG 2 0.2063 Tier II
28 NC_000077.6 11 61889018 + Akap10 NNNAAGATCAAGTCCAGCAC NGG 2 0.15 Tier II
29 NC_000069.6 3 66186639 + Veph1 NNNAACATCAAGGCCAGTCC NGG 2 0.133 Tier II
30 NC_000077.6 11 51595011 - Phykpl NNNAACATCAAGGCCACCAA NGG 2 0.1176 Tier II
31 NC_000082.6 16 35632494 - Sema5b NNNACCATCAAGGCCACCAC NGG 2 0.1176 Tier II
32 NC_000071.6 5 62758146 - Arap2 NNNAACATCAAGGCCAACAG NGG 2 0.0549 Tier II
33 NC_000071.6 5 44663801 + Ldb2 NNNGACATCAAGGCCAGCAC NGA 2 0.0434 Tier II
34 NC_000069.6 3 54743916 - Alg5 NNNAACATCAGGGCCAGCAC NTG 2 0.0253 Tier II
35 NC_000073.6 7 130453325 - Ate1 NNNAACATCATGGCTAGCAC NGG 2 0.0205 Tier II
36 NC_000082.6 16 34823767 + Mylk NNNAACATCAAGGCCAGCAT NTG 2 0.0117 Tier II
37 NC_000071.6 5 76958148 - Paics NNNAACATCAAGGCAAGCAC NTG 2 0.0087 Tier II
38 NC_000080.6 14 30899519 - Itih4 NNNAACATCATGGCCAGCAC NGC 2 0.0068 Tier II
39 NC_000077.6 11 62998299 - Cdrt4os1 NNNAACATGAAGGCCAGAAC NGG 2 0.3333 Tier III
40 NC_000077.6 11 62998299 - Gm46284 NNNAACATGAAGGCCAGAAC NGG 2 0.3333 Tier III
41 NC_000074.6 8 124076959 - Gm30005 NNNATCATCAAGGCCAGAAC NGG 2 0.1958 Tier III
42 NC_000081.6 15 24984053 + Gm2824 NNNAACATGAAGGCCAGCAT NGG 2 0.1857 Tier III
43 NC_000078.6 12 71625326 + 9630002D21Rik NNNGACATCAAGGCCAGGAC NGG 2 0.0833 Tier III
44 NC_000073.6 7 142549360 + Nctc1 NNNAACATCAAGGCCAGCCT NGG 2 0.0621 Tier III
45 NC_000082.6 16 93442804 + 1700029J03Rik NNNAACATCAAGGGCATCAC NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)