Construct: sgRNA BRDN0001145402

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCCTTCAAGAAAGGCGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRC (6714)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75650

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 37386149 + SRC NNNCCTTCAAGAAAGGCGAG NGG 0 1.0 Tier I
2 NC_000016.10 16 27268648 - NSMCE1 NNNACTTCAAGGAAGGCGAG NGG 2 0.6082 Tier II
3 NC_000017.11 17 893518 + NXN NNNCCTTCAAAAAAGGCCAG NGG 2 0.4762 Tier II
4 NC_000019.10 19 32699274 + NUDT19 NNNCCTCAAAGAAAGGCGAG NGG 2 0.4469 Tier II
5 NC_000003.12 3 151325298 - MED12L NNNCCTTCAAGAAAAGAGAG NGG 2 0.4392 Tier II
6 NC_000001.11 1 25990454 + PAFAH2 NNNCCCTCAAGAAAGGTGAG NGG 2 0.4242 Tier II
7 NC_000003.12 3 113398408 + CFAP44 NNNCCTTCCAGAAAGGCAAG NGG 2 0.3956 Tier II
8 NC_000011.10 11 121573039 - SORL1 NNNCCTCCAGGAAAGGCGAG NGG 2 0.3819 Tier II
9 NC_000010.11 10 70776611 - TBATA NNNCCTCCAAGAAAGGCCAG NGG 2 0.3274 Tier II
10 NC_000005.10 5 134739093 - CAMLG NNNCCTTCAAGAAAGGCGAG NAG 1 0.2593 Tier II
11 NC_000005.10 5 14710342 + ANKH NNNCCTTCAGGAAAGGCGAG NGA 2 0.0386 Tier II
12 NC_000005.10 5 14710342 + OTULIN NNNCCTTCAGGAAAGGCGAG NGA 2 0.0386 Tier II
13 NC_000022.11 22 25105691 - KIAA1671 NNNCCTTCCAGAAAGGGGAG NGG 2 0.0336 Tier II
14 NC_000005.10 5 124651322 + ZNF608 NNNCCTTCAGGAAAGGGGAG NGG 2 0.0327 Tier II
15 NC_000005.10 5 171182213 + RANBP17 NNNCCTTCAAGAAAGGCGTG NGT 2 0.0087 Tier II
16 NC_000016.10 16 27268648 - NSMCE1-DT NNNACTTCAAGGAAGGCGAG NGG 2 0.6082 Tier III
17 NC_000011.10 11 7912137 + LOC107984307 NNNCCATCAAGAAAGGCAAG NGG 2 0.6 Tier III
18 NC_000011.10 11 10332206 + CAND1.11 NNNCCTTCAAGAAAGGCTAT NGG 2 0.4667 Tier III
19 NC_000019.10 19 32699274 + LOC124904690 NNNCCTCAAAGAAAGGCGAG NGG 2 0.4469 Tier III
20 NC_000003.12 3 113398408 + SPICE1-CFAP44 NNNCCTTCCAGAAAGGCAAG NGG 2 0.3956 Tier III
21 NC_000009.12 9 82639400 + LOC107987087 NNNCCTGCAAGAAAGGCTAG NGG 2 0.3922 Tier III
22 NC_000014.9 14 34169344 - LOC102724945 NNNCCTTCAAGGAAGGAGAG NGG 2 0.337 Tier III
23 NC_000001.11 1 194288935 - LOC107985242 NNNCCTTCAAGAAAGGAGGG NGG 2 0.175 Tier III
24 NC_000014.9 14 61569501 - LINC01303 NNNCCTTCAAGAAAGAGGAG NGG 2 0.0588 Tier III
25 NC_000022.11 22 25105691 - KIAA1671-AS1 NNNCCTTCCAGAAAGGGGAG NGG 2 0.0336 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 157458608 + Src NNNCCTTCAAGAAAGGGGAG NGG 1 0.0588 Tier I
2 NC_000085.6 19 44785813 - Pax2 NNNCCTTCAAGAAAGACTAG NGG 2 0.6667 Tier II
3 NC_000070.6 4 88258041 - Focad NNNCCTCCAAGAAAGGTGAG NGG 2 0.3208 Tier II
4 NC_000068.7 2 127498380 - Kcnip3 NNNCCTTAAAGAAAGGAGAG NGG 2 0.3033 Tier II
5 NC_000083.6 17 17882618 - Fpr1 NNNCTTTCAAGAAAGGAGAG NGG 2 0.297 Tier II
6 NC_000067.6 1 70382326 + Spag16 NNNCCTGCAAGAAAGGAGAG NGG 2 0.2745 Tier II
7 NC_000071.6 5 100155668 + Tmem150c NNNCATTCAAGAAAGGTGAG NGG 2 0.2667 Tier II
8 NC_000076.6 10 69962500 + Ank3 NNNGCTTCAAGAAAGGTGAG NGG 2 0.2333 Tier II
9 NC_000086.7 X 95168355 + Arhgef9 NNNCCTTCAAGAAAGGTGAG NAG 2 0.121 Tier II
10 NC_000079.6 13 55623547 - Caml NNNCCTTCAAGAAAGGCGAG NAA 2 0.0 Tier II
11 NC_000080.6 14 54075557 + Tcra NNNCCTTCAAGAAAGGCTAG NGT 2 0.0108 Tier III
12 NC_000080.6 14 54075557 + Tcrd NNNCCTTCAAGAAAGGCTAG NGT 2 0.0108 Tier III
13 NC_000080.6 14 54075557 + Gm30275 NNNCCTTCAAGAAAGGCTAG NGT 2 0.0108 Tier III
Download CSV

Other clones with same target sequence:

(none)