Construct: sgRNA BRDN0001145402
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTCCTTCAAGAAAGGCGAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- SRC (6714)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000020.11 | 20 | 37386149 | + | SRC | NNNCCTTCAAGAAAGGCGAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000016.10 | 16 | 27268648 | - | NSMCE1 | NNNACTTCAAGGAAGGCGAG | NGG | 2 | 0.6082 | Tier II |
3 | NC_000017.11 | 17 | 893518 | + | NXN | NNNCCTTCAAAAAAGGCCAG | NGG | 2 | 0.4762 | Tier II |
4 | NC_000019.10 | 19 | 32699274 | + | NUDT19 | NNNCCTCAAAGAAAGGCGAG | NGG | 2 | 0.4469 | Tier II |
5 | NC_000003.12 | 3 | 151325298 | - | MED12L | NNNCCTTCAAGAAAAGAGAG | NGG | 2 | 0.4392 | Tier II |
6 | NC_000001.11 | 1 | 25990454 | + | PAFAH2 | NNNCCCTCAAGAAAGGTGAG | NGG | 2 | 0.4242 | Tier II |
7 | NC_000003.12 | 3 | 113398408 | + | CFAP44 | NNNCCTTCCAGAAAGGCAAG | NGG | 2 | 0.3956 | Tier II |
8 | NC_000011.10 | 11 | 121573039 | - | SORL1 | NNNCCTCCAGGAAAGGCGAG | NGG | 2 | 0.3819 | Tier II |
9 | NC_000010.11 | 10 | 70776611 | - | TBATA | NNNCCTCCAAGAAAGGCCAG | NGG | 2 | 0.3274 | Tier II |
10 | NC_000005.10 | 5 | 134739093 | - | CAMLG | NNNCCTTCAAGAAAGGCGAG | NAG | 1 | 0.2593 | Tier II |
11 | NC_000005.10 | 5 | 14710342 | + | ANKH | NNNCCTTCAGGAAAGGCGAG | NGA | 2 | 0.0386 | Tier II |
12 | NC_000005.10 | 5 | 14710342 | + | OTULIN | NNNCCTTCAGGAAAGGCGAG | NGA | 2 | 0.0386 | Tier II |
13 | NC_000022.11 | 22 | 25105691 | - | KIAA1671 | NNNCCTTCCAGAAAGGGGAG | NGG | 2 | 0.0336 | Tier II |
14 | NC_000005.10 | 5 | 124651322 | + | ZNF608 | NNNCCTTCAGGAAAGGGGAG | NGG | 2 | 0.0327 | Tier II |
15 | NC_000005.10 | 5 | 171182213 | + | RANBP17 | NNNCCTTCAAGAAAGGCGTG | NGT | 2 | 0.0087 | Tier II |
16 | NC_000016.10 | 16 | 27268648 | - | NSMCE1-DT | NNNACTTCAAGGAAGGCGAG | NGG | 2 | 0.6082 | Tier III |
17 | NC_000011.10 | 11 | 7912137 | + | LOC107984307 | NNNCCATCAAGAAAGGCAAG | NGG | 2 | 0.6 | Tier III |
18 | NC_000011.10 | 11 | 10332206 | + | CAND1.11 | NNNCCTTCAAGAAAGGCTAT | NGG | 2 | 0.4667 | Tier III |
19 | NC_000019.10 | 19 | 32699274 | + | LOC124904690 | NNNCCTCAAAGAAAGGCGAG | NGG | 2 | 0.4469 | Tier III |
20 | NC_000003.12 | 3 | 113398408 | + | SPICE1-CFAP44 | NNNCCTTCCAGAAAGGCAAG | NGG | 2 | 0.3956 | Tier III |
21 | NC_000009.12 | 9 | 82639400 | + | LOC107987087 | NNNCCTGCAAGAAAGGCTAG | NGG | 2 | 0.3922 | Tier III |
22 | NC_000014.9 | 14 | 34169344 | - | LOC102724945 | NNNCCTTCAAGGAAGGAGAG | NGG | 2 | 0.337 | Tier III |
23 | NC_000001.11 | 1 | 194288935 | - | LOC107985242 | NNNCCTTCAAGAAAGGAGGG | NGG | 2 | 0.175 | Tier III |
24 | NC_000014.9 | 14 | 61569501 | - | LINC01303 | NNNCCTTCAAGAAAGAGGAG | NGG | 2 | 0.0588 | Tier III |
25 | NC_000022.11 | 22 | 25105691 | - | KIAA1671-AS1 | NNNCCTTCCAGAAAGGGGAG | NGG | 2 | 0.0336 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 157458608 | + | Src | NNNCCTTCAAGAAAGGGGAG | NGG | 1 | 0.0588 | Tier I |
2 | NC_000085.6 | 19 | 44785813 | - | Pax2 | NNNCCTTCAAGAAAGACTAG | NGG | 2 | 0.6667 | Tier II |
3 | NC_000070.6 | 4 | 88258041 | - | Focad | NNNCCTCCAAGAAAGGTGAG | NGG | 2 | 0.3208 | Tier II |
4 | NC_000068.7 | 2 | 127498380 | - | Kcnip3 | NNNCCTTAAAGAAAGGAGAG | NGG | 2 | 0.3033 | Tier II |
5 | NC_000083.6 | 17 | 17882618 | - | Fpr1 | NNNCTTTCAAGAAAGGAGAG | NGG | 2 | 0.297 | Tier II |
6 | NC_000067.6 | 1 | 70382326 | + | Spag16 | NNNCCTGCAAGAAAGGAGAG | NGG | 2 | 0.2745 | Tier II |
7 | NC_000071.6 | 5 | 100155668 | + | Tmem150c | NNNCATTCAAGAAAGGTGAG | NGG | 2 | 0.2667 | Tier II |
8 | NC_000076.6 | 10 | 69962500 | + | Ank3 | NNNGCTTCAAGAAAGGTGAG | NGG | 2 | 0.2333 | Tier II |
9 | NC_000086.7 | X | 95168355 | + | Arhgef9 | NNNCCTTCAAGAAAGGTGAG | NAG | 2 | 0.121 | Tier II |
10 | NC_000079.6 | 13 | 55623547 | - | Caml | NNNCCTTCAAGAAAGGCGAG | NAA | 2 | 0.0 | Tier II |
11 | NC_000080.6 | 14 | 54075557 | + | Tcra | NNNCCTTCAAGAAAGGCTAG | NGT | 2 | 0.0108 | Tier III |
12 | NC_000080.6 | 14 | 54075557 | + | Tcrd | NNNCCTTCAAGAAAGGCTAG | NGT | 2 | 0.0108 | Tier III |
13 | NC_000080.6 | 14 | 54075557 | + | Gm30275 | NNNCCTTCAAGAAAGGCTAG | NGT | 2 | 0.0108 | Tier III |