Construct: sgRNA BRDN0001145403
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGCTTGAGGGATTCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HCK (3055)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77216
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
32084485 |
- |
HCK |
NNNGCTTGAGGGATTCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
47802831 |
+ |
TPRX1 |
NNNGCTGGAAGGATTCCCGA |
NGG |
2 |
0.549 |
Tier I |
| 3 |
NC_000015.10 |
15 |
26914847 |
+ |
GABRA5 |
NNNGCTTGAGGACTTCCCGA |
NGG |
2 |
0.1965 |
Tier I |
| 4 |
NC_000002.12 |
2 |
124265230 |
- |
CNTNAP5 |
NNNGCTTAAGGGATTCCAGA |
NGG |
2 |
0.5385 |
Tier II |
| 5 |
NC_000010.11 |
10 |
89488719 |
+ |
SLC16A12 |
NNNGTTTGGGGGATTCCCGA |
NGG |
2 |
0.4242 |
Tier II |
| 6 |
NC_000021.9 |
21 |
34504211 |
+ |
KCNE1 |
NNNGTTTGAGGGATTCCAGA |
NGG |
2 |
0.3427 |
Tier II |
| 7 |
NC_000001.11 |
1 |
18937820 |
- |
IFFO2 |
NNNGCTTGAGGGATTCCCCT |
NGG |
2 |
0.269 |
Tier II |
| 8 |
NC_000006.12 |
6 |
41689133 |
+ |
TFEB |
NNNGCTTGAGGGATTTCCTA |
NGG |
2 |
0.2051 |
Tier II |
| 9 |
NC_000004.12 |
4 |
85658713 |
- |
ARHGAP24 |
NNNGCTTGAGTGATTCCCCA |
NGG |
2 |
0.1724 |
Tier II |
| 10 |
NC_000016.10 |
16 |
88922612 |
+ |
CBFA2T3 |
NNNGCTTGAGGCCTTCCCGA |
NGG |
2 |
0.1115 |
Tier II |
| 11 |
NC_000009.12 |
9 |
112959660 |
+ |
MUPP |
NNNGCTTGAGGGATTACCAA |
NGG |
2 |
0.7143 |
Tier III |
| 12 |
NC_000009.12 |
9 |
87016570 |
- |
LINC02893 |
NNNGCTTGAGAGATTCCCTA |
NGG |
2 |
0.6667 |
Tier III |
| 13 |
NC_000001.11 |
1 |
67875298 |
+ |
GNG12-AS1 |
NNNGCTTGCTGGATTCCCGA |
NGG |
2 |
0.4643 |
Tier III |
| 14 |
NC_000013.11 |
13 |
73610987 |
- |
LINC00393 |
NNNGCTTGAGGCATTCCCAA |
NGG |
2 |
0.3782 |
Tier III |
| 15 |
NC_000013.11 |
13 |
73610987 |
- |
LOC105370256 |
NNNGCTTGAGGCATTCCCAA |
NGG |
2 |
0.3782 |
Tier III |
| 16 |
NC_000002.12 |
2 |
168347880 |
+ |
LOC105373734 |
NNNGCTGGAGGGAATCCCGA |
NGG |
2 |
0.3641 |
Tier III |
| 17 |
NC_000007.14 |
7 |
26388384 |
+ |
SNX10-AS1 |
NNNGCTTGAGGCATTCCCCA |
NGG |
2 |
0.2373 |
Tier III |
| 18 |
NC_000004.12 |
4 |
85658713 |
- |
LOC105377319 |
NNNGCTTGAGTGATTCCCCA |
NGG |
2 |
0.1724 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
66094824 |
+ |
Ephx2 |
NNNGCTTGAGGGATTCCCTA |
NAG |
2 |
0.1728 |
Tier I |
| 2 |
NC_000083.6 |
17 |
15555216 |
+ |
Prdm9 |
NNNGCTTCAGGGATGCCCGA |
NGG |
2 |
0.0308 |
Tier I |
| 3 |
NC_000079.6 |
13 |
44877237 |
+ |
Jarid2 |
NNNCCATGAGGGATTCCCGA |
NGG |
2 |
0.4588 |
Tier II |
| 4 |
NC_000078.6 |
12 |
79626332 |
+ |
Rad51b |
NNNGCTTAAGGGATTCCCCA |
NGG |
2 |
0.4483 |
Tier II |
| 5 |
NC_000072.6 |
6 |
128030259 |
- |
Tspan9 |
NNNGCTTGAGGGATTCTAGA |
NGG |
2 |
0.2513 |
Tier II |
| 6 |
NC_000086.7 |
X |
144406886 |
+ |
Trpc5 |
NNNGCTTGAGGGCTTCCCAA |
NGG |
2 |
0.1504 |
Tier II |
| 7 |
NC_000078.6 |
12 |
53777769 |
- |
Npas3 |
NNNGCTTGAGGGATTTACGA |
NGG |
2 |
0.1436 |
Tier II |
| 8 |
NC_000081.6 |
15 |
11113310 |
- |
Adamts12 |
NNNGCTTGAGGGATGCCTGA |
NGG |
2 |
0.0321 |
Tier II |
| 9 |
NC_000077.6 |
11 |
120348974 |
+ |
0610009L18Rik |
NNNGCTGGAGGGATTCCCGG |
NGG |
2 |
0.4498 |
Tier III |
Other clones with same target sequence:
(none)
