Construct: sgRNA BRDN0001145410
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGTAAAACTTATCAAACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K4 (6416)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76651
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
12107830 |
- |
MAP2K4 |
NNNGTAAAACTTATCAAACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
43396642 |
- |
ANO10 |
NNNATAAAACTTATCAAACA |
NGG |
2 |
0.8438 |
Tier II |
| 3 |
NC_000003.12 |
3 |
189739945 |
- |
TP63 |
NNNGTAAAACATATCAAACA |
NGG |
2 |
0.7031 |
Tier II |
| 4 |
NC_000009.12 |
9 |
70205882 |
+ |
MAMDC2 |
NNNGTAAAACTTGTCAAACA |
NGG |
2 |
0.6114 |
Tier II |
| 5 |
NC_000003.12 |
3 |
133225983 |
- |
TMEM108 |
NNNATAAAACTTATAAAACG |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000010.11 |
10 |
88474927 |
- |
RNLS |
NNNGTAAAACTTCTCAAACA |
NGG |
2 |
0.1974 |
Tier II |
| 7 |
NC_000001.11 |
1 |
237059790 |
- |
RYR2 |
NNNGTAAAAATTATCAACCG |
NGG |
2 |
0.1651 |
Tier II |
| 8 |
NC_000023.11 |
X |
111159426 |
+ |
PAK3 |
NNNGTAAAACTTATCAACCC |
NGG |
2 |
0.0816 |
Tier II |
| 9 |
NC_000006.12 |
6 |
131023101 |
- |
EPB41L2 |
NNNGTAAAACTTATCTAACG |
NAG |
2 |
0.0 |
Tier II |
| 10 |
NC_000010.11 |
10 |
64653829 |
- |
LOC124902439 |
NNNATAAAACTTATCAAATG |
NGG |
2 |
0.4154 |
Tier III |
| 11 |
NC_000023.11 |
X |
73525603 |
+ |
MAP2K4P1 |
NNNGTAAAACTTATCAAACG |
NGA |
1 |
0.0694 |
Tier III |
| 12 |
NC_000001.11 |
1 |
184758595 |
+ |
LOC105371649 |
NNNCTAAAACTTATCAAAGG |
NGG |
2 |
0.0662 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
65712258 |
+ |
Map2k4 |
NNNGTAAAACTTATCGAACG |
NGG |
1 |
0.1923 |
Tier I |
| 2 |
NC_000083.6 |
17 |
15400261 |
- |
Fam120b |
NNNTTAAAACTTATCAAACT |
NGG |
2 |
0.2545 |
Tier II |
| 3 |
NC_000086.7 |
X |
10291541 |
- |
Otc |
NNNTTAAAACTTATCAAAAG |
NGG |
2 |
0.1558 |
Tier II |
| 4 |
NC_000073.6 |
7 |
141102859 |
+ |
Ano9 |
NNNGTAAAACTTAGCAAGCG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
