Construct: sgRNA BRDN0001145417
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGACTTGACGTGGAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPKAPK2 (9261)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77209
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
206685365 |
- |
MAPKAPK2 |
NNNGACTTGACGTGGAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
64074379 |
+ |
RGS19 |
NNNGACTTGGCGTGGCACTG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000019.10 |
19 |
41276722 |
- |
HNRNPUL1 |
NNNAACTTGACATGGAACTG |
NGG |
2 |
0.84 |
Tier II |
| 4 |
NC_000016.10 |
16 |
2107704 |
+ |
PKD1 |
NNNGACTGTACGTGGAACTG |
NGG |
2 |
0.3911 |
Tier II |
| 5 |
NC_000003.12 |
3 |
129089035 |
+ |
RAB43 |
NNNGACTTGCCCTGGAACTG |
NGG |
2 |
0.1765 |
Tier II |
| 6 |
NC_000003.12 |
3 |
129089035 |
+ |
ISY1-RAB43 |
NNNGACTTGCCCTGGAACTG |
NGG |
2 |
0.1765 |
Tier II |
| 7 |
NC_000014.9 |
14 |
81020556 |
+ |
TSHR |
NNNGACCTGAGGTGGAACTG |
NGG |
2 |
0.1719 |
Tier II |
| 8 |
NC_000011.10 |
11 |
1956756 |
+ |
MRPL23 |
NNNGAGTTGACGGGGAACTG |
NGG |
2 |
0.1304 |
Tier II |
| 9 |
NC_000020.11 |
20 |
62250131 |
- |
OSBPL2 |
NNNGCCTTGAGGTGGAACTG |
NGG |
2 |
0.125 |
Tier II |
| 10 |
NC_000002.12 |
2 |
74532563 |
+ |
HTRA2 |
NNNGACTTGAGGTGGAACTC |
NGG |
2 |
0.1071 |
Tier II |
| 11 |
NC_000002.12 |
2 |
74532563 |
+ |
LOXL3 |
NNNGACTTGAGGTGGAACTC |
NGG |
2 |
0.1071 |
Tier II |
| 12 |
NC_000018.10 |
18 |
61909040 |
+ |
LOC112268209 |
NNNGACTTGACATTGAACTG |
NGG |
2 |
0.2489 |
Tier III |
| 13 |
NC_000016.10 |
16 |
46624338 |
+ |
RAB43P1 |
NNNGACTTGCCCTGGAACTG |
NGG |
2 |
0.1765 |
Tier III |
| 14 |
NC_000014.9 |
14 |
81020556 |
+ |
LOC101928462 |
NNNGACCTGAGGTGGAACTG |
NGG |
2 |
0.1719 |
Tier III |
| 15 |
NC_000011.10 |
11 |
34709903 |
+ |
NDUFB8P3 |
NNNGACTTGAGGTTGAACTG |
NGG |
2 |
0.0667 |
Tier III |
| 16 |
NC_000011.10 |
11 |
34709903 |
+ |
LOC102723568 |
NNNGACTTGAGGTTGAACTG |
NGG |
2 |
0.0667 |
Tier III |
| 17 |
NC_000002.12 |
2 |
820407 |
- |
LINC01115 |
NNNGACATGACGTGGTACTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
131097433 |
+ |
Mapkapk2 |
NNNGACTTGACGTGGAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
84079909 |
- |
Rfx1 |
NNNGGCTTGCCGTGGAACTG |
NGG |
2 |
0.24 |
Tier I |
| 3 |
NC_000080.6 |
14 |
59355910 |
- |
Phf11d |
NNNGACCAGACGTGGAACTG |
NGG |
2 |
0.55 |
Tier II |
| 4 |
NC_000080.6 |
14 |
59388865 |
- |
Phf11c |
NNNGACCAGACGTGGAACTG |
NGG |
2 |
0.55 |
Tier II |
| 5 |
NC_000080.6 |
14 |
59388865 |
- |
Gm45935 |
NNNGACCAGACGTGGAACTG |
NGG |
2 |
0.55 |
Tier II |
| 6 |
NC_000071.6 |
5 |
89042440 |
+ |
Slc4a4 |
NNNGTCTTGAAGTGGAACTG |
NGG |
2 |
0.2727 |
Tier II |
| 7 |
NC_000072.6 |
6 |
141270681 |
+ |
Pde3a |
NNNGACTTGATGTTGAACTG |
NGG |
2 |
0.0821 |
Tier II |
| 8 |
NC_000076.6 |
10 |
118517567 |
- |
Ifngas1 |
NNNGACTAGAAGTGGAACTG |
NGG |
2 |
0.6 |
Tier III |
Other clones with same target sequence:
(none)
