Construct: sgRNA BRDN0001145418
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGACATAGTTGGCCCACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GALK1 (2584)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76705
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
75763937 |
+ |
GALK1 |
NNNACATAGTTGGCCCACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
45923597 |
- |
CLIC5 |
NNNACAAAGTTGGCCCACAG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000010.11 |
10 |
88839381 |
- |
ANKRD22 |
NNNACACAGTTGGCCCACTG |
NGG |
2 |
0.3173 |
Tier II |
| 4 |
NC_000013.11 |
13 |
24752648 |
+ |
RNF17 |
NNNACATAGATGGCCCGCCG |
NGG |
2 |
0.1513 |
Tier II |
| 5 |
NC_000014.9 |
14 |
50813154 |
- |
NIN |
NNNACATAGTTAGCCCACCG |
NTG |
2 |
0.0364 |
Tier II |
| 6 |
NC_000016.10 |
16 |
69291436 |
+ |
SNTB2 |
NNNACATAGTTGGCCCAGCG |
NTG |
2 |
0.0052 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
116011067 |
+ |
Galk1 |
NNNACATAATTGGCCCACTG |
NGG |
2 |
0.2967 |
Tier I |
| 2 |
NC_000071.6 |
5 |
112283967 |
+ |
Tpst2 |
NNNACATAGTAGGCCCACAG |
NGG |
2 |
0.3214 |
Tier II |
| 3 |
NC_000076.6 |
10 |
24925350 |
- |
Arg1 |
NNNACATAGTTGGCCTACCT |
NGG |
2 |
0.2154 |
Tier II |
| 4 |
NC_000074.6 |
8 |
3206947 |
+ |
Insr |
NNNACATAGTTGGCCCCACG |
NGG |
2 |
0.095 |
Tier II |
| 5 |
NC_000070.6 |
4 |
129953175 |
+ |
Spocd1 |
NNNACACAGTTGGCCCACGG |
NGG |
2 |
0.0859 |
Tier II |
| 6 |
NC_000077.6 |
11 |
58109625 |
+ |
Cnot8 |
NNNACATAGTTGGCTCACGG |
NGG |
2 |
0.0083 |
Tier II |
| 7 |
NC_000076.6 |
10 |
24925350 |
- |
Gm40616 |
NNNACATAGTTGGCCTACCT |
NGG |
2 |
0.2154 |
Tier III |
Other clones with same target sequence:
(none)
