Construct: sgRNA BRDN0001145424

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAAATATAAGTGCCACCACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM7 (54822)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76113

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 50634535 + TRPM7 NNNATATAAGTGCCACCACA NGG 0 1.0 Tier I
2 NC_000006.12 6 143573685 - PHACTR2 NNNAGATAAGTGCCAACACA NGG 2 0.64 Tier II
3 NC_000023.11 X 75467247 - ZDHHC15 NNNATTAAAGTGCCACCACA NGG 2 0.625 Tier II
4 NC_000012.12 12 5969521 + VWF NNNATGTAAGTCCCACCACA NGG 2 0.3782 Tier II
5 NC_000006.12 6 165793701 - PDE10A NNNATCTAAGTGCCACCACT NGG 2 0.2727 Tier II
6 NC_000015.10 15 78575695 - CHRNA5 NNNATATAAGTGCCAGCACA NGG 1 0.1538 Tier II
7 NC_000014.9 14 22577699 - DAD1 NNNATATAAGTGAAACCACA NGG 2 0.1346 Tier II
8 NC_000020.11 20 20539858 + RALGAPA2 NNNATATAAGTGACACCACA NAG 2 0.0997 Tier II
9 NC_000007.14 7 108266224 - NRCAM NNNATAGAAGTGCCAGCACA NGG 2 0.0905 Tier II
10 NC_000019.10 19 46074201 + IGFL4 NNNATATTAGTGCCTCCACA NGG 2 0.0857 Tier II
11 NC_000004.12 4 110480404 + ENPEP NNNATATAAGGGCCACCAGA NGG 2 0.05 Tier II
12 NC_000016.10 16 70137436 - PDPR NNNATATAAGTGGAACCACA NGG 2 0.0477 Tier II
13 NC_000009.12 9 97664275 - NCBP1 NNNATATATGTGCCACCACA NTG 2 0.0234 Tier II
14 NC_000009.12 9 97664275 - XPA NNNATATATGTGCCACCACA NTG 2 0.0234 Tier II
15 NC_000008.11 8 81852585 - LINC02235 NNNATATAATTGCCACCACA NGG 1 0.8125 Tier III
16 NC_000013.11 13 38642563 - LINC00437 NNNATATATTTGCCACCACA NGG 2 0.4875 Tier III
17 NC_000002.12 2 207206254 + MYOSLID-AS1 NNNATATTAGTGCCACCACA NGG 1 0.4286 Tier III
18 NC_000007.14 7 44999451 - LOC102723334 NNNATAGAAGTGCAACCACA NGG 2 0.2059 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 100787286 + Gxylt2 NNNATAAAAGTGCCATCACA NGG 2 0.2692 Tier I
2 NC_000069.6 3 129868532 - Cfi NNNATATAAATGCCACCACA NGT 2 0.0151 Tier I
3 NC_000068.7 2 25037601 + Pnpla7 NNNAGACAAGTGCCACCACA NGG 2 0.44 Tier II
4 NC_000070.6 4 85225953 - Sh3gl2 NNNACATAAGTGCCACCAAA NGG 2 0.4286 Tier II
5 NC_000080.6 14 39177524 + Nrg3 NNNATCTAAGTGCTACCACA NGG 2 0.3333 Tier II
6 NC_000080.6 14 31293569 + Dnah1 NNNAAAGAAGTGCCACCACA NGG 2 0.2941 Tier II
7 NC_000082.6 16 45712834 - Tagln3 NNNAAAGAAGTGCCACCACA NGG 2 0.2941 Tier II
8 NC_000067.6 1 24521560 + Col19a1 NNNATATGAGTGACACCACA NGG 2 0.2821 Tier II
9 NC_000073.6 7 141135724 - Ptdss2 NNNATGTAAGTGCCATCACA NGG 2 0.2198 Tier II
10 NC_000075.6 9 56475833 + Hmg20a NNNATATTAGTGACACCACA NGG 2 0.1648 Tier II
11 NC_000072.6 6 38868950 + Hipk2 NNNATATAAGTGACACCACA NGA 2 0.0267 Tier II
12 NC_000072.6 6 38868950 + Tbxas1 NNNATATAAGTGACACCACA NGA 2 0.0267 Tier II
13 NC_000067.6 1 36382383 - Fer1l5 NNNATATATGTGCCACCACA NGC 2 0.0133 Tier II
14 NC_000077.6 11 42155150 - Gabra1 NNNATCTAAGTGCCACCACA NGT 2 0.0073 Tier II
15 NC_000080.6 14 31293569 + Gm35823 NNNAAAGAAGTGCCACCACA NGG 2 0.2941 Tier III
16 NC_000070.6 4 87644382 + Gm34180 NNNATCTAAGTGACACCACA NGG 2 0.1748 Tier III
17 NC_000067.6 1 64164746 + Gm35880 NNNATATTAGGGCCACCACA NGG 2 0.1714 Tier III
18 NC_000076.6 10 67378946 - Gm46230 NNNATATAAGTCCCACCACC NGG 2 0.1203 Tier III
Download CSV

Other clones with same target sequence:

(none)