Construct: sgRNA BRDN0001145430
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACGAGCTGCTTCACGGTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CLK4 (57396)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77797
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
178613796 |
+ |
CLK4 |
NNNGAGCTGCTTCACGGTAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
13410807 |
- |
TASP1 |
NNNGAGCTGCTTCACAGGAA |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000001.11 |
1 |
161726238 |
- |
FCRLB |
NNNGCGCTGCTTCACGGCAA |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000002.12 |
2 |
111050505 |
- |
ACOXL |
NNNGAGCTGCTTCAAGGTCA |
NGG |
2 |
0.046 |
Tier II |
| 5 |
NC_000001.11 |
1 |
165854734 |
- |
UCK2 |
NNNGAGCTGCTTCAGCGTAA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
51275259 |
- |
Clk4 |
NNNGAGCTGCCTCCCGGTAA |
NGG |
2 |
0.1429 |
Tier I |
| 2 |
NC_000081.6 |
15 |
89215804 |
- |
Ppp6r2 |
NNNGAGCTGCTCCACGGTAA |
NGG |
1 |
0.9474 |
Tier II |
| 3 |
NC_000074.6 |
8 |
119271034 |
+ |
Cdh13 |
NNNGAGCTGCTTCTCAGTAA |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000068.7 |
2 |
73529326 |
- |
Wipf1 |
NNNGAGCCGCTGCACGGTAA |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000086.7 |
X |
152017658 |
- |
Smc1a |
NNNGAGCTACTTCAAGGTAA |
NGG |
2 |
0.1429 |
Tier II |
| 6 |
NC_000084.6 |
18 |
38739704 |
- |
Arhgap26 |
NNNGAGCTGCTTCACGGTCA |
NAG |
2 |
0.0536 |
Tier II |
| 7 |
NC_000086.7 |
X |
130066646 |
- |
Diaph2 |
NNNGAGCTGCTACATGGTAA |
NGG |
2 |
0.0533 |
Tier II |
| 8 |
NC_000072.6 |
6 |
137998678 |
- |
Slc15a5 |
NNNGAGCTGCTTCAGGGTAT |
NGG |
2 |
0.03 |
Tier II |
| 9 |
NC_000086.7 |
X |
20910062 |
- |
Syn1 |
NNNGTGCTGCTTCATGGTAA |
NGG |
2 |
0.0242 |
Tier II |
| 10 |
NC_000067.6 |
1 |
163863048 |
- |
Kifap3 |
NNNGAGCTGCTTCACAGTAA |
NGT |
2 |
0.0161 |
Tier II |
| 11 |
NC_000072.6 |
6 |
136408303 |
+ |
E330021D16Rik |
NNNGAGCTGCCTCACTGTAA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000071.6 |
5 |
54083178 |
+ |
Stim2 |
NNNGAGCTGCTTCTCTGTAA |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000069.6 |
3 |
146642187 |
- |
Gm16325 |
NNNGAGCTGCTTCTCGGTAA |
NGA |
2 |
0.037 |
Tier III |
| 14 |
NC_000085.6 |
19 |
23074393 |
- |
C330002G04Rik |
NNNGAGCTGCTTCACGGTAC |
NGC |
2 |
0.0051 |
Tier III |
Other clones with same target sequence:
(none)
