Construct: sgRNA BRDN0001145436

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTTGTGGCCAATATGCTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRPS2 (5634)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78008

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 12809274 + PRPS2 NNNTGTGGCCAATATGCTGT NGG 0 1.0 Tier I
2 NC_000013.11 13 77096466 - MYCBP2 NNNTGTGGCCGATATGCTGG NGG 2 0.1147 Tier I
3 NC_000023.11 X 151179810 + GPR50 NNNTGTGGCCGATATGCTGG NGG 2 0.1147 Tier I
4 NC_000007.14 7 36221922 - EEPD1 NNNTGTGGCCAGTGTGCTGT NGG 2 0.337 Tier II
5 NC_000001.11 1 94048228 - ABCA4 NNNTGTGGCCAAAATGCTCT NGG 2 0.3103 Tier II
6 NC_000001.11 1 50486733 - FAF1 NNNTGTGGACATTATGCTGT NGG 2 0.2857 Tier II
7 NC_000015.10 15 90880623 + FURIN NNNTGTGGCCCATGTGCTGT NGG 2 0.1867 Tier II
8 NC_000008.11 8 130825874 - ADCY8 NNNTGTGGCCACTATGCTGA NGG 2 0.148 Tier II
9 NC_000010.11 10 103774617 + SH3PXD2A NNNTGTGGCTAATATGCTGT NGA 2 0.0654 Tier II
10 NC_000012.12 12 51255689 + SMAGP NNNTGTGGACAATAGGCTGT NGG 2 0.0429 Tier II
11 NC_000003.12 3 140317498 - CLSTN2 NNNTGTGGCCAACAGGCTGT NGG 2 0.0395 Tier II
12 NC_000001.11 1 171976902 + DNM3 NNNTGTGGCCAAGATTCTGT NGG 2 0.0 Tier II
13 NC_000007.14 7 132624003 - PLXNA4 NNNTGTGGCCAATATGCTGT NAC 2 0.0 Tier II
14 NC_000006.12 6 2324491 + GMDS-DT NNNTGTGACCAAAATGCTGT NGG 2 0.6923 Tier III
15 NC_000024.10 Y 7727595 + RFTN1P1 NNNAGTGGCCAAGATGCTGT NGG 2 0.1242 Tier III
16 NC_000005.10 5 129203408 + LOC102723654 NNNTGTGCCCAATATGCTGC NGG 2 0.0559 Tier III
17 NC_000001.11 1 67626057 - LOC124904197 NNNTGTGGCAAATAGGCTGT NGG 2 0.0433 Tier III
18 NC_000007.14 7 24230075 + LOC107986777 NNNTGTGGCCAAGATGCTGC NGG 2 0.0237 Tier III
19 NC_000015.10 15 81688033 - LOC105370921 NNNTGTGGCCAATATGCTGT NGT 1 0.0161 Tier III
20 NC_000007.14 7 140666122 - LOC105375535 NNNTGTGGCCAATAGGCTGT NTG 2 0.0019 Tier III
21 NC_000011.10 11 77573438 - LINC03030 NNNTGTGGCCAATAGGCTGT NTG 2 0.0019 Tier III
22 NC_000003.12 3 102606161 - LOC105374016 NNNTGTGGCCAATATGCTGT NAC 2 0.0 Tier III
23 NC_000005.10 5 58192228 + LOC105378983 NNNTGTGGCCATTATCCTGT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 22146483 + Zfp947 NNNTGTGGATAATATGCTGT NGG 2 0.8067 Tier I
2 NC_000083.6 17 21881043 + Zfp995 NNNCGTGGACAATATGCTGT NGG 2 0.5546 Tier I
3 NC_000086.7 X 167365178 - Prps2 NNNTGTGGCCAATATGCTGT NAG 1 0.2593 Tier I
4 NC_000080.6 14 103154900 - Mycbp2 NNNTGTGGCCGATATGCTGG NGG 2 0.1147 Tier I
5 NC_000069.6 3 15514536 - Sirpb1b NNNTGTGGACAATATGCTGT NGG 1 0.8571 Tier II
6 NC_000071.6 5 9462741 + Elapor2 NNNTGAGGCAAATATGCTGT NGG 2 0.7511 Tier II
7 NC_000079.6 13 20422361 + Elmo1 NNNTGTGGAAAATATGCTGT NGG 2 0.7429 Tier II
8 NC_000072.6 6 5131129 + Ppp1r9a NNNTGTGGGCAATGTGCTGT NGG 2 0.2889 Tier II
9 NC_000075.6 9 80110162 - Senp6 NNNTGTTGCCAATATGCTGT NAG 2 0.1481 Tier II
10 NC_000076.6 10 64793478 - Ctnna3 NNNTTTGGCCAATGTGCTGT NGG 2 0.14 Tier II
11 NC_000077.6 11 107625249 - Helz NNNTGTGGCCAATCTGATGT NGG 2 0.1 Tier II
12 NC_000076.6 10 82680332 + Glt8d2 NNNTGTGGCCAATATGATGT NTG 2 0.0182 Tier II
13 NC_000067.6 1 84523829 - Dner NNNTGTGGCCAACATGCTGT NGT 2 0.0127 Tier II
14 NC_000069.6 3 15514536 - Gm21014 NNNTGTGGACAATATGCTGT NGG 1 0.8571 Tier III
15 NC_000083.6 17 22068850 + Gm7818 NNNTGTGGACAATATGTTGT NGG 2 0.4 Tier III
16 NC_000072.6 6 116798729 - Gm36873 NNNTATGGCCAAGATGCTGT NGG 2 0.2261 Tier III
17 NC_000077.6 11 51200166 - Platr20 NNNTGTGGCCAATAGGCTGA NGG 2 0.0281 Tier III
18 NC_000069.6 3 87966762 + Gm3745 NNNTGTGGCCCATATGCTGT NTG 2 0.0156 Tier III
19 NC_000068.7 2 48433591 + Gm13481 NNNTGTTGCCAATATGCTGT NGC 2 0.0127 Tier III
Download CSV

Other clones with same target sequence:

(none)