Construct: sgRNA BRDN0001145441
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCACGAGGAGTTCAGGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HKDC1 (80201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76829
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
69239091 |
+ |
HKDC1 |
NNNACGAGGAGTTCAGGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
133946626 |
+ |
IGSF9B |
NNNACAAGGAGTCCAGGACA |
NGG |
2 |
0.7895 |
Tier II |
| 3 |
NC_000015.10 |
15 |
52503424 |
+ |
MYO5A |
NNNTCAAGGAGTTCAGGACA |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000007.14 |
7 |
71420626 |
- |
GALNT17 |
NNNAAGAGGAGTTCAGAACA |
NGG |
2 |
0.5333 |
Tier II |
| 5 |
NC_000001.11 |
1 |
41977227 |
+ |
HIVEP3 |
NNNACAAGGAGTTCAGGATA |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000008.11 |
8 |
103574436 |
- |
RIMS2 |
NNNAGCAGGAGTTCAGGACA |
NGG |
2 |
0.4091 |
Tier II |
| 7 |
NC_000010.11 |
10 |
103031695 |
+ |
CNNM2 |
NNNAGCAGGAGTTCAGGACA |
NGG |
2 |
0.4091 |
Tier II |
| 8 |
NC_000010.11 |
10 |
80420362 |
- |
PRXL2A |
NNNACCAGGAGGTCAGGACA |
NGG |
2 |
0.3409 |
Tier II |
| 9 |
NC_000005.10 |
5 |
15917412 |
+ |
FBXL7 |
NNNCCCAGGAGTTCAGGACA |
NGG |
2 |
0.2406 |
Tier II |
| 10 |
NC_000012.12 |
12 |
8126437 |
- |
CLEC4A |
NNNCCCAGGAGTTCAGGACA |
NGG |
2 |
0.2406 |
Tier II |
| 11 |
NC_000003.12 |
3 |
62745290 |
- |
CADPS |
NNNACGAGGAGTTCAAGACC |
NGG |
2 |
0.2273 |
Tier II |
| 12 |
NC_000003.12 |
3 |
122634650 |
+ |
PARP15 |
NNNATGAGGAGTTAAGGACA |
NGG |
2 |
0.2227 |
Tier II |
| 13 |
NC_000012.12 |
12 |
39807195 |
+ |
SLC2A13 |
NNNATGAGGAGTTAAGGACA |
NGG |
2 |
0.2227 |
Tier II |
| 14 |
NC_000012.12 |
12 |
39807195 |
+ |
REDIC1 |
NNNATGAGGAGTTAAGGACA |
NGG |
2 |
0.2227 |
Tier II |
| 15 |
NC_000006.12 |
6 |
36246513 |
- |
PNPLA1 |
NNNAGGAGGAGTTAAGGACA |
NGG |
2 |
0.21 |
Tier II |
| 16 |
NC_000021.9 |
21 |
40751676 |
+ |
DSCAM |
NNNACAAGGAGTTCAGGAGA |
NGG |
2 |
0.125 |
Tier II |
| 17 |
NC_000016.10 |
16 |
67930650 |
- |
CTRL |
NNNACGAGGAATTCAGGACA |
NGC |
2 |
0.0222 |
Tier II |
| 18 |
NC_000010.11 |
10 |
37250476 |
+ |
ANKRD30A |
NNNACGAGGGGTTCAGGACA |
NTG |
2 |
0.0216 |
Tier II |
| 19 |
NC_000016.10 |
16 |
2517233 |
+ |
ATP6V0C |
NNNACCAGGAGTTCAGGACA |
NGC |
2 |
0.0152 |
Tier II |
| 20 |
NC_000003.12 |
3 |
52047088 |
+ |
LOC124906238 |
NNNAGGAGGAGCTCAGGACA |
NGG |
2 |
0.5684 |
Tier III |
| 21 |
NC_000006.12 |
6 |
36246513 |
- |
LOC105375036 |
NNNAGGAGGAGTTAAGGACA |
NGG |
2 |
0.21 |
Tier III |
| 22 |
NC_000002.12 |
2 |
858875 |
- |
LINC01115 |
NNNACGAGGAGATCAGGACA |
NAG |
2 |
0.2074 |
Tier III |
| 23 |
NC_000010.11 |
10 |
37250476 |
+ |
ATP8A2P1 |
NNNACGAGGGGTTCAGGACA |
NTG |
2 |
0.0216 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
89814462 |
+ |
Agap1 |
NNNACAAGGAGTCCAGGACA |
NGG |
2 |
0.7895 |
Tier II |
| 2 |
NC_000078.6 |
12 |
53449506 |
- |
Npas3 |
NNNACCAAGAGTTCAGGACA |
NGG |
2 |
0.6818 |
Tier II |
| 3 |
NC_000077.6 |
11 |
119240076 |
- |
Ccdc40 |
NNNGCGAGGAGATCAGGACA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000067.6 |
1 |
97897588 |
- |
Pam |
NNNACGGGAAGTTCAGGACA |
NGG |
2 |
0.4538 |
Tier II |
| 5 |
NC_000083.6 |
17 |
65608154 |
+ |
Vapa |
NNNACGAGGAATGCAGGACA |
NGG |
2 |
0.2609 |
Tier II |
| 6 |
NC_000077.6 |
11 |
5138992 |
- |
Emid1 |
NNNAGGAGGATTTCAGGACA |
NGG |
2 |
0.2308 |
Tier II |
| 7 |
NC_000080.6 |
14 |
57956059 |
- |
Micu2 |
NNNACGAGGAGTTCATGAGA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000067.6 |
1 |
157080404 |
- |
Gm30568 |
NNNACGAGGAGGGCAGGACA |
NGG |
2 |
0.1304 |
Tier III |
| 9 |
NC_000075.6 |
9 |
57313450 |
- |
Gm39343 |
NNNACTAGGAGTTCAGGACA |
NGA |
2 |
0.0463 |
Tier III |
Other clones with same target sequence:
(none)
