Construct: sgRNA BRDN0001145443

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAAACATATCCATTAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA6 (285220)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76248

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 96866885 + EPHA6 NNNAACATATCCATTAAATG NGG 0 1.0 Tier I
2 NC_000007.14 7 33480379 + BBS9 NNNAACATAACCATTAAATG NGG 1 0.8571 Tier II
3 NC_000008.11 8 63068470 + TTPA NNNAATATGTCCATTAAATG NGG 2 0.619 Tier II
4 NC_000007.14 7 27651298 + HIBADH NNNAACACTTCCATTAAATG NGG 2 0.6 Tier II
5 NC_000001.11 1 172567625 - SUCO NNNTAAATATCCATTAAATG NGG 2 0.5909 Tier II
6 NC_000015.10 15 76843114 + SCAPER NNNAACACATCCATAAAATG NGG 2 0.5789 Tier II
7 NC_000010.11 10 129513032 + MGMT NNNAACAGATACATTAAATG NGG 2 0.55 Tier II
8 NC_000010.11 10 129513032 + LOC124902531 NNNAACAGATACATTAAATG NGG 2 0.55 Tier II
9 NC_000017.11 17 30079443 - EFCAB5 NNNAACATATCCATAAATTG NGG 2 0.2895 Tier II
10 NC_000016.10 16 26045843 - HS3ST4 NNNAAAATATGCATTAAATG NGG 2 0.2321 Tier II
11 NC_000001.11 1 84867036 + LPAR3 NNNAACATATCTATTAAGTG NGG 2 0.2154 Tier II
12 NC_000005.10 5 54132282 - ARL15 NNNAACATATTCATTAAATT NGG 2 0.2154 Tier II
13 NC_000007.14 7 82095915 - CACNA2D1 NNNAACATCTCCATTAAAAG NGG 2 0.1633 Tier II
14 NC_000012.12 12 32307403 - BICD1 NNNAACATTTCCATTACATG NGG 2 0.1059 Tier II
15 NC_000007.14 7 3748458 + SDK1 NNNAAAATATCCATTAAATG NCG 2 0.0995 Tier II
16 NC_000007.14 7 80823804 - SEMA3C NNNAACATATCCATGAAATA NGG 2 0.0469 Tier II
17 NC_000012.12 12 68881415 + CPM NNNAACATATCCACTAAATG NGA 2 0.0198 Tier II
18 NC_000017.11 17 12141513 + MAP2K4 NNNAACATATTCATGAAATG NGG 2 0.0154 Tier II
19 NC_000005.10 5 244894 + SDHA NNNAACATATGCATGAAATG NGG 2 0.0125 Tier II
20 NC_000009.12 9 87500356 - DAPK1 NNNAACATATCCATGAAATG NGA 2 0.0035 Tier II
21 NC_000018.10 18 34169512 - NOL4 NNNAACATATACATTTAATG NGG 2 0.0 Tier II
22 NC_000005.10 5 96350999 + CAST NNNAACATATCCATTTAATG NGA 2 0.0 Tier II
23 NC_000010.11 10 32931375 - ITGB1 NNNAACATATCCATTTTATG NGG 2 0.0 Tier II
24 NC_000005.10 5 55469681 + PLPP1 NNNAACATTTCCATTTAATG NGG 2 0.0 Tier II
25 NC_000018.10 18 74933834 + ZNF407 NNNAATATATCCATTTAATG NGG 2 0.0 Tier II
26 NC_000004.12 4 183303169 - WWC2 NNNTACATATCCATTTAATG NGG 2 0.0 Tier II
27 NC_000011.10 11 61190599 - LOC124902678 NNNAACAAAACCATTAAATG NGG 2 0.6857 Tier III
28 NC_000007.14 7 27651298 + LOC105375211 NNNAACACTTCCATTAAATG NGG 2 0.6 Tier III
29 NC_000009.12 9 32778136 + LOC105376017 NNNAACATATAAATTAAATG NGG 2 0.5357 Tier III
30 NC_000015.10 15 52875046 - LOC107983981 NNNAACACAGCCATTAAATG NGG 2 0.5 Tier III
31 NC_000023.11 X 36395514 - LOC101928627 NNNAACAAACCCATTAAATG NGG 2 0.4267 Tier III
32 NC_000018.10 18 71595312 + LOC107985179 NNNAACATATAGATTAAATG NGG 2 0.3333 Tier III
33 NC_000011.10 11 60829266 - LOC105369322 NNNAACAAATCCATTAAGTG NGG 2 0.32 Tier III
34 NC_000003.12 3 151048744 - CLRN1-AS1 NNNAACATTCCCATTAAATG NGG 2 0.32 Tier III
35 NC_000007.14 7 82095915 - LOC124901687 NNNAACATCTCCATTAAAAG NGG 2 0.1633 Tier III
36 NC_000001.11 1 193515407 - LOC124904475 NNNAACATATCCAATAAATG NAG 2 0.1605 Tier III
37 NC_000014.9 14 45403977 - LOC105370476 NNNAACATATGCATTAAATG NTG 2 0.0097 Tier III
38 NC_000011.10 11 19287355 + CSRP3-AS1 NNNAACATATCCATTAGATG NTG 2 0.0069 Tier III
39 NC_000008.11 8 93304376 - LOC107986956 NNNAACATATCCATGAAATG NTG 2 0.0019 Tier III
40 NC_000018.10 18 34169512 - LOC124904279 NNNAACATATACATTTAATG NGG 2 0.0 Tier III
41 NC_000005.10 5 96350999 + LOC101929710 NNNAACATATCCATTTAATG NGA 2 0.0 Tier III
42 NC_000018.10 18 74933834 + LOC124904323 NNNAATATATCCATTTAATG NGG 2 0.0 Tier III
43 NC_000003.12 3 34320724 - LINC01811 NNNATCATATCCATTTAATG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000082.6 16 60526454 - Epha6 NNNAACATATCCATTAAATG NGG 0 1.0 Tier I
2 NC_000075.6 9 108798337 + Ip6k2 NNNAGCATATCCATTAAAAG NGG 2 0.2057 Tier I
3 NC_000086.7 X 137982974 + Il1rapl2 NNNAACATCTCCATTAAATG NGG 1 0.5714 Tier II
4 NC_000067.6 1 175117425 - Rgs7 NNNAGCATATCCATTAAATC NGG 2 0.3086 Tier II
5 NC_000082.6 16 63687456 + Epha3 NNNAAAATATCCTTTAAATG NGG 2 0.2786 Tier II
6 NC_000075.6 9 7114033 + Dync2h1 NNNAACACATCCATCAAATG NGG 2 0.2727 Tier II
7 NC_000074.6 8 62959774 - Spock3 NNNAATATATCCATTAAAAG NGG 2 0.2653 Tier II
8 NC_000077.6 11 95754688 + Zfp652 NNNAACACATCCATTAAATG NAG 2 0.2593 Tier II
9 NC_000071.6 5 66339512 - Apbb2 NNNAACATAACCATTAAATG NAG 2 0.2222 Tier II
10 NC_000077.6 11 33780582 + Kcnip1 NNNCACATCTCCATTAAATG NGG 2 0.2017 Tier II
11 NC_000086.7 X 130379344 - Diaph2 NNNAACATGTCCTTTAAATG NGG 2 0.2 Tier II
12 NC_000068.7 2 58305256 - Acvr1c NNNAACATATACATTAAATG NAG 2 0.1944 Tier II
13 NC_000070.6 4 86207506 - Adamtsl1 NNNAACATATCCAATAAAAG NGG 2 0.1769 Tier II
14 NC_000075.6 9 101238388 + Ppp2r3a NNNAACATTTCCACTAAATG NGG 2 0.1714 Tier II
15 NC_000076.6 10 33426623 + Trdn NNNAACATATCCAACAAATG NGG 2 0.1688 Tier II
16 NC_000073.6 7 128755116 - Sec23ip NNNAATATATCCATTACATG NGG 2 0.1639 Tier II
17 NC_000082.6 16 57042442 + Tmem45a2 NNNAAAATATCCATTATATG NGG 2 0.1238 Tier II
18 NC_000068.7 2 68694052 - 4932414N04Rik NNNAACAAATCCATTAAATG NGA 2 0.0556 Tier II
19 NC_000078.6 12 103116130 - Unc79 NNNAACATATCCATCATATG NGG 2 0.0364 Tier II
20 NC_000076.6 10 11382209 + Epm2a NNNAACATATCCAATAAATG NTG 2 0.0241 Tier II
21 NC_000075.6 9 62119799 + Glce NNNAACACATCCATTAAATG NGC 2 0.0222 Tier II
22 NC_000073.6 7 60196165 + Snrpn NNNAACATATACAGTAAATG NGG 2 0.0 Tier II
23 NC_000070.6 4 65615981 + Astn2 NNNAACATATCCAGCAAATG NGG 2 0.0 Tier II
24 NC_000070.6 4 65615981 + Trim32 NNNAACATATCCAGCAAATG NGG 2 0.0 Tier II
25 NC_000078.6 12 117503687 + Gm31482 NNNAACAGTTCCATTAAATG NGG 2 0.44 Tier III
26 NC_000076.6 10 14756661 - 1700016L04Rik NNNCACATATCCATAAAATG NGG 2 0.2043 Tier III
27 NC_000074.6 8 76787085 + Gm10649 NNNAACATATCCATTGAATG NAG 2 0.0499 Tier III
28 NC_000073.6 7 60196165 + Snhg14 NNNAACATATACAGTAAATG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)